TRAF-6 Antibody

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Western Blot: TRAF-6 Antibody [NB100-56436] - Analysis of TRAF-6 using NB100-56436 in A) human thymus cell lysate and B) human testis cell lysate.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, RMSpecies Glossary
Applications WB, Flow
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

TRAF-6 Antibody Summary

Immunogen
Anti-TRAF6 polyclonal antibody was raised against a peptide corresponding to amino acids between 410 and 460 of human TRAF6.
Predicted Species
Mouse (94%), Rat (94%), Porcine (98%), Bovine (98%), Rhesus Macaque (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRAF6
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 3-5 ug/ml
  • Flow Cytometry
Control
TRAF-6 Overexpression Lysate
Publications
Read Publications using NB100-56436.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: sooty mangabey (Cercocebus atys) (98%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.1 ml PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for TRAF-6 Antibody

  • E3 ubiquitin-protein ligase TRAF6
  • EC 6.3.2.-
  • Interleukin-1 signal transducer
  • RING finger protein 85
  • RNF85MGC:3310
  • TNF receptor-associated factor 6
  • TRAF6
  • TRAF-6

Background

Tumor necrosis factor (TNF) induced signaling is mediated through association of TNF receptor (TNFR) with adaptor proteins, such as TNF receptor associated proteins (TRAFs). TRAFs form a family of cytoplasmic adapter proteins that mediate signal transduction from many members of the TNF-receptor superfamily (e.g. RANK, CD30, CD40, etc.) and the interleukin-1 receptor. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. Recent molecular cloning studies have lead to identification of six TRAFs (TRAF1-TRAF6) (1-4). Recently it has been shown that TRANCE/OPGL activates the antiapoptotic serine/threonine kinase Akt/PKB through a signaling complex involving c-Src and TRAF6 (5). Mice deficient in TNF receptor-associated factor 6 (TRAF6) are osteopetrotic with defects in bone remodeling and tooth eruption due to impaired osteoclast function (6). Like TRAF2 and TRAF3, TRAF6 is also essential for perinatal and postnatal survival. These findings establish unexpectedly diverse and critical roles for TRAF6 in perinatal and postnatal survival, bone metabolism, LPS, and cytokine signaling. RANK (TRANCE receptor) interacts with various TRAFs through distinct motifs and activates NF-kB via a novel TRAF6 interaction motif, which then activates NIK, thus leading to NF-kB activation (7). Over-expression of TRAF6 activates JNK, p38, or IKK in the absence of extracellular stimulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TRAF-6 Antibody (NB100-56436)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol TRAF6
Entrez
Uniprot