TRAF-6 Antibody - BSA Free

Western Blot: TRAF-6 Antibody [NB100-56179] - Analysis of TRAF-6 in normal Human tissues using this antibody. TRAF6 is observed at ~66 kDa. Additional bands of lower molecular weight were seen in some cases, and may represent TRAF-6 degradation fragments.

Immunohistochemistry-Paraffin: TRAF-6 Antibody [NB100-56179] - Stained for TRAF6 using this antibody at 1:2000. Hematoxylin-eosin counterstain. A, mouse spleen. B, mouse thymus.

Immunohistochemistry-Paraffin: TRAF-6 Antibody [NB100-56179] - 1:2000. Analysis Hematoxylin-eosin counterstain. A, mouse lymph node. B, mouse bone marrow.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TRAF-6 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide corresponding to amino acids 451-469 (DAKPELLAFQRPTIPRNPK) of human TRAF6 was used as immunogen, GenBank no. NP_665802.1.
• Specificity: TRAF6
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRAF6
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200
  • Western Blot 1:1000-1:2000
• Control:

  TRAF-6 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for TRAF-6 Antibody - BSA Free

• E3 ubiquitin-protein ligase TRAF6
• EC 6.3.2.-
• Interleukin-1 signal transducer
• RING finger protein 85
• RNF85MGC:3310
• TNF receptor-associated factor 6
• TRAF6
• TRAF-6

Background

The TRAF (TNF receptor-associated factor) family is a group of adapter proteins (TRAFs 1-6) that link a wide variety of cell surface receptors to diverse signaling cascades leading to the activation of NF-kB and mitogen-activated protein kinases (reviewed in Chung et al, 2002). TRAFs are major signal transducers for both the TNF and IL- 1/TLR receptor superfamilies and collectively play important functions in both adaptive and innate immunity. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. TRAFs interact with a variety of proteins that regulate receptor-induced cell death or survival, and TRAF-mediated signaling can promote cell survival or interfere with death receptor-induced apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRAF-6 Antibody (NB100-56179)(5)

Publications using NB100-56179

• Wei F, Ba S, Jin M et al. RNF180 Inhibits Proliferation and Promotes Apoptosis of Colorectal Cancer Through Ubiquitination of WISP1 Frontiers in Cell and Developmental Biology 2021-01-22 [PMID: 33553163] (Western Blot, Immunohistochemistry-Paraffin)
• Wang P, Liu BY, Wu MM et al. Moderate prenatal alcohol exposure suppresses the TLR4-mediated innate immune response in the hippocampus of young rats Neurosci. Lett. 2019-01-30 [PMID: 30710662] (WB, IF/IHC, Rat)
• Wei W, Wan H, Peng X et al. Antiviral effects of Ma Huang Tang against H1N1 influenza virus infection in vitro and in an ICR pneumonia mouse model Biomedicine & Pharmacotherapy 2018-06-01 [PMID: 29710534] (IF/IHC, Mouse)
• Ye Y, Yang Y, Chen C et al. Electroacupuncture Improved Hippocampal Neurogenesis following Traumatic Brain Injury in Mice through Inhibition of TLR4 Signaling Pathway Stem Cells Int. 2017-08-06 [PMID: 28848607] (WB, Mouse)
• Zapata JM, Krajewska M, Krajewski S et al. TNFR-associated factor family protein expression in normal tissues and lymphoid malignancies. J Immunol. 2000-11-01 [PMID: 11046039] (WB, IHC-P)
  
  Details:
  WB: Figs 1, 2, 4, 5; IHC-paraffin: Fig 3.

Bioinformatics

• Gene Symbol: TRAF6
• Entrez:
  • 7189(Human)
  • 22034(Mouse)
  • 311245(Rat)