Cadherin-13 Antibody [Unconjugated]

Western blot shows lysates of human brain (cortex) tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Cadherin‑13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3264) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Cadherin‑13 at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Cadherin‑13 was detected in immersion fixed NCI-H460 human large cell lung carcinoma cell line using 10 µg/mL Goat Anti-Human Cadherin‑13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3264) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Cadherin‑13 was detected in immersion fixed paraffin-embedded sections of human skin using Goat Anti-Human Cadherin‑13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3264) at 0.1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of human brain (motor cortex) tissue, loaded at 0.2 mg/mL. A specific band was detected for Cadherin‑13 at approximately 114 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Cadherin‑13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3264) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Cadherin-13 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Cadherin-13
  Glu23-Ala692
  Accession # P55290
• Specificity: Detects human Cadherin-13 in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross-reactivity with recombinant mouse Cadherin-13 is observed, and less than 10% cross-reactivity with recombinant human (rh) N-Cadherin is observed, and less than 2% cross-reactivity with rhCadherin-8, rhCadherin-11, rhCadherin-17, rhE-Cadherin, rhP-Cadherin, rhVE-Cadherin and rhR-Cadherin is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CDH13
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cadherin-13 Antibody [Unconjugated]

• cadherin 13, H-cadherin (heart)
• Cadherin13
• Cadherin-13
• CDH13
• CDHHT-cadherin
• H-Cadherin
• Heart cadherin
• heart-cadherin
• P105
• T-cad
• T-Cadherin
• Truncated cadherin
• truncated-cadherin

Background

Cadherin-13, also known as T-Cadherin and H-Cadherin, is a 105 kDa member of the cadherin family of transmembrane glycoproteins that mediate calcium-dependent intercellular adhesion (1). However, Cadherin-13 is an atypical member, lacking transmembrane and cytosolic domains and containing a GPI moiety that anchors Cadherin-13 to the plasma membrane (1‑2). Human Cadherin-13 is synthesized as a 713 amino acid (aa) precursor that contains a 22 aa signal sequence, a 116 aa propeptide, a 555 aa mature chain, and a second propeptide of 20 aa that is removed in the mature form to reveal the GPI anchor. The mature form contains five cadherin domains and eight potential sites for N-linked glycosylation. Mature human Cadherin-13 shares 96% aa identity with mature mouse Cadherin-13. Cadherin-13 is expressed in various tissues. It is highly expressed in the heart, and in the CNS, Cadherin-13 is expressed in the cerebral cortex, medulla, hippocampus, amygdala, thalamus, and substantia nigra (2). There are higher levels of Cadherin-13 in the adult brain than in developing brain (2). Cadherin‑13 is also expressed in skin in the basal layer of the epidermis, lung, liver, kidney, and blood vessels (2). The structural characteristics of Cadherin-13 predict that it is unlikely to function as a true adhesion molecule in vivo (2). It is suggested that it may act rather as a signaling receptor participating in recognition of the environment and regulation of cell motility, proliferation, and phenotype (2). Cellular expression levels of Cadherin‑13 in various tissues often correlate, negatively or positively, with the proliferative potential of the cells (2). Cadherin-13 may also act as a suppressor of tumor cell growth (2). This potential role for Cadherin‑13 was emphasized by localization of Cadherin-13 gene to chromosome 16q24, a region exhibiting loss of heterozygosity in many solid tumors (2). Allelic loss of chromosome bands 16q24.1-q24.2 and reduced expression of Cadherin‑13, as well as hypermethylation of the remaining allele have been detected in a considerable number of human cancers (2).

1. Tanihara, H. et al. (1994) Cell Adhes. Commun. 2:15.
2. Philippova, M. et al. (2009) Cell. Signal. 21:1035.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cadherin-13 Antibody (AF3264)(11)

Publications using AF3264

• MR Vitale, JEM Zöller, C Jansch, A Janz, F Edenhofer, E Klopocki, D van den Ho, T Vanmierlo, O Rivero, N Nadif Kasr, GC Ziegler, KP Lesch Generation of induced pluripotent stem cell (iPSC) lines carrying a heterozygous (UKWMPi002-A-1) and null mutant knockout (UKWMPi002-A-2) of Cadherin 13 associated with neurodevelopmental disorders using CRISPR/Cas9 Stem Cell Research, 2021-01-11;51(0):102169. 2021-01-11 [PMID: 33486346] (ICC, Western Blot, Human)
• Y Nakamura, S Kita, Y Tanaka, S Fukuda, Y Obata, T Okita, H Nishida, Y Takahashi, Y Kawachi, Y Tsugawa-Sh, Y Fujishima, H Nishizawa, Y Takakura, S Miyagawa, Y Sawa, N Maeda, I Shimomura Adiponectin Stimulates Exosome Release to Enhance Mesenchymal Stem-Cell-Driven Therapy of Heart Failure in Mice Mol. Ther., 2020-07-10;0(0):. 2020-07-10 [PMID: 32652045] (Western Blot, Human)
• D Mori, S Miyagawa, R Matsuura, N Sougawa, S Fukushima, T Ueno, K Toda, T Kuratani, K Tomita, N Maeda, I Shimomura, Y Sawa Pioglitazone strengthen therapeutic effect of adipose-derived regenerative cells against ischemic cardiomyopathy through enhanced expression of adiponectin and modulation of macrophage phenotype Cardiovasc Diabetol, 2019-03-22;18(1):39. 2019-03-22 [PMID: 30902059] (Western Blot, Rat)
• Y Obata, S Kita, Y Koyama, S Fukuda, H Takeda, M Takahashi, Y Fujishima, H Nagao, S Masuda, Y Tanaka, Y Nakamura, H Nishizawa, T Funahashi, B Ranscht, Y Izumi, T Bamba, E Fukusaki, R Hanayama, S Shimada, N Maeda, I Shimomura Adiponectin/T-cadherin system enhances exosome biogenesis and decreases cellular ceramides by exosomal release JCI Insight, 2018-04-19;3(8):. 2018-04-19 [PMID: 29669945] (ICC, Western Blot, Mouse)
• A Frismantie, E Kyriakakis, B Dasen, P Erne, TJ Resink, M Philippova Actin cytoskeleton regulates functional anchorage-migration switch during T-cadherin-induced phenotype modulation of vascular smooth muscle cells Cell Adh Migr, 2017-05-25;0(0):1-17. 2017-05-25 [PMID: 28524745] (Western Blot, Human)
• S Fukuda, S Kita, Y Obata, Y Fujishima, H Nagao, S Masuda, Y Tanaka, H Nishizawa, T Funahashi, J Takagi, N Maeda, I Shimomura The unique prodomain of T-cadherin plays a key role in adiponectin binding with the essential extracellular cadherin repeats 1 and 2 J. Biol. Chem, 2017-03-21;0(0):. 2017-03-21 [PMID: 28325833] (Western Blot, Mouse)
• Georgia M Alexander Vagal Nerve Stimulation Modifies Neuronal Activity and the Proteome of Excitatory Synapses of Amygdala/Piriform Cortex J. Neurochem, 2017-02-03;0(0):. 2017-02-03 [PMID: 27973753] (Western Blot, Rat)
• Stanislaw Buechner T-Cadherin Expression in the Epidermis and Adnexal Structures of Normal Skin Dermatopathology (Basel), 2016-10-21;3(4):68-78. 2016-10-21 [PMID: 27904857] (IHC, Human)
• Parker-Duffen , Jennifer, Nakamura , Kazuto, Silver , Marcy, Kikuchi , Ryosuke, Tigges , Ulrich, Yoshida , Sumiko, Denzel , Martin S, Ranscht , Barbara, Walsh , Kenneth T-cadherin is essential for adiponectin-mediated revascularization. J Biol Chem, 2013-07-03;288(34):24886-97. 2013-07-03 [PMID: 23824191] (IHC-Fr, Immunoprecipitation, Western Blot, Mouse)
• Kyriakakis E, Maslova K, Frachet A, Ferri N, Contini A, Pfaff D, Erne P, Resink T, Philippova M Cross-talk between EGFR and T-cadherin: EGFR activation promotes T-cadherin localization to intercellular contacts. Cell Signal, 2013-02-11;25(5):1044-53. 2013-02-11 [PMID: 23411345] (ICC, Western Blot, Human)
• Philippova M, Ivanov D, Joshi MB, Kyriakakis E, Rupp K, Afonyushkin T, Bochkov V, Erne P, Resink TJ Identification of proteins associating with glycosylphosphatidylinositol- anchored T-cadherin on the surface of vascular endothelial cells: role for Grp78/BiP in T-cadherin-dependent cell survival. Mol. Cell. Biol., 2008-04-14;28(12):4004-17. 2008-04-14 [PMID: 18411300] (ICC, Western Blot, Human)

Bioinformatics

• Gene Symbol: CDH13
• Uniprot:
  • P55290()