TORC3/CRTC3 Antibody (JE63-18)

Western Blot: TORC3/CRTC3 Antibody (JE63-18) [NBP3-32226] - Western blot analysis of TORC3/CRTC3 on different lysates with Rabbit anti-TORC3/CRTC3 antibody (NBP3-32226) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: TT cell lysate Lane 3: Jurkat cell lysate Lane 4: HDLM-2 cell lysate Lane 5: HEK-293 cell lysate Lane 6: COS-1 cell lysate Lane 7: RAW264.7 cell lysate Lane 8: NIH/3T3 cell lysate Lane 9: PC-12 cell lysate Lane 10: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 67 kDa Observed band size: 75 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32226) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: TORC3/CRTC3 Antibody (JE63-18) [NBP3-32226] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-TORC3/CRTC3 antibody (NBP3-32226) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32226) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: TORC3/CRTC3 Antibody (JE63-18) [NBP3-32226] - Flow cytometric analysis of TT cells labeling TORC3/CRTC3. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32226, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, Flow, IHC
• Clone: JE63-18
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

TORC3/CRTC3 Antibody (JE63-18) Summary

• Description: Novus Biologicals Rabbit TORC3/CRTC3 Antibody (JE63-18) (NBP3-32226) is a recombinant monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide within Human TORC3/CRTC3 aa 570-219 / 619. (Uniprot: Q6UUV7)
• Localization: Nucleus, Cytoplasm.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CRTC3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:500
  • Western Blot 1:2000
• Theoretical MW: 67 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for TORC3/CRTC3 Antibody (JE63-18)

• CREB regulated transcription coactivator 3
• CRTC3
• FLJ21868
• TORC3
• TORC-3
• TORC3CREB-regulated transcription coactivator 3
• Transducer of CREB protein 3
• transducer of regulated cAMP response element-binding protein (CREB) 3
• Transducer of regulated cAMP response element-binding protein 3
• transducer of regulated CREB protein 3

Background

Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMPresponse element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active whendephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 withTAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potentcoactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activationof the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CRTC3