CRTC3 Antibodies
We offer TORC3/CRTC3 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot. Each TORC3/CRTC3 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our TORC3/CRTC3 Antibodies can be used in a variety of model species: Human, Mouse, Primate. Use the list below to choose the TORC3/CRTC3 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our TORC3/CRTC3 polyclonal antibodies and browse our TORC3/CRTC3 monoclonal antibody catalog.
Alternate Names for CRTC3 Antibodies
anti-TORC3/CRTC3 antibody, anti-CRTC3 antibody, anti-CREB regulated transcription coactivator 3 antibody, anti-FLJ21868 antibody, anti-TORC-3 antibody, anti-TORC3CREB-regulated transcription coactivator 3 antibody, anti-Transducer of CREB protein 3 antibody, anti-transducer of regulated cAMP response element-binding protein (CREB) 3 antibody, anti-Transducer of regulated cAMP response element-binding protein 3 antibody, anti-transducer of regulated CREB protein 3 antibody
19 Results for "torc3-crtc3" in Primary Antibodies
HAF005). A specific band was detected for TORC3 at approximately 83 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse TORC3 Monoclonal Antibody (Catalog # MAB6840) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for TORC3 at approximately 83 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="TORC3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human TORC3 Monoclonal Antibody (Catalog # MAB9455) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />
![Immunohistochemistry: TORC3/CRTC3 Antibody (JE63-18) [NBP3-32226] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-TORC3/CRTC3 antibody (NBP3-32226) at 1/500 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32226) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32226_rabbit-torc3-crtc3-mab-je63-18-298202414304117.jpg "Immunohistochemistry: TORC3/CRTC3 Antibody (JE63-18) [NBP3-32226] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-TORC3/CRTC3 antibody (NBP3-32226) at 1/500 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32226) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Flow Cytometry: TORC3/CRTC3 Antibody (JE63-18) [NBP3-32226] - Flow cytometric analysis of TT cells labeling TORC3/CRTC3.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32226, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32226_rabbit-torc3-crtc3-mab-je63-18-2920241645324.jpg)
![Western blot analysis on different lysates with NBP3-32226 at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: TT cell lysate Lane 3: Jurkat cell lysate Lane 4: HDLM-2 cell lysate Lane 5: HEK-293 cell lysate Lane 6: COS-1 cell lysate Lane 7: RAW264.7 cell lysate Lane 8: NIH/3T3 cell lysate Lane 9: PC-12 cell lysate Lane 10: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 67 kDa Observed band size: 75 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32226 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32226_-western-blot-639204597143331121.jpg)
![Immunohistochemistry-Paraffin: TORC3/CRTC3 Antibody [NBP1-46832] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti- TORC3 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/TORC3-CRTC3-Antibody-Immunohistochemistry-Paraffin-NBP1-46832-img0004.jpg "Immunohistochemistry-Paraffin: TORC3/CRTC3 Antibody [NBP1-46832] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti- TORC3 used at a dilution of 1:200 (1ug/ml). Detection: DAB")
![Immunoprecipitation: TORC3/CRTC3 Antibody [NBP1-46832] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-TORC3 antibody used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. TORC3 was also immunoprecipitated by rabbit anti-TORC3 antibody BL9829, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/TORC3-CRTC3-Antibody-Immunoprecipitation-NBP1-46832-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: TORC3/CRTC3 Antibody [NBP1-83615] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/TORC3-CRTC3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83615-img0004.jpg "Immunocytochemistry/Immunofluorescence: TORC3/CRTC3 Antibody [NBP1-83615] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.")
![Immunohistochemistry-Paraffin: TORC3/CRTC3 Antibody [NBP1-83615] - Staining of human liver shows strong cytoplasmic, membranous and nuclear positivity in hepatocytes.](http://images.novusbio.com/fullsize/TORC3-CRTC3-Antibody-Immunohistochemistry-Paraffin-NBP1-83615-img0002.jpg)
![Western Blot: TORC3/CRTC3 Antibody (S07-4C9) [NBP3-20014] - Western blot detection of TORC3/CRTC3 in K562, Rat Brain, C6, 3T3, Hela cell lysates using NBP3-20014 (1:1000 diluted). Predicted band size: 67kDa. Observed band size: 78kDa.](http://images.novusbio.com/fullsize/TORC3-CRTC3-Antibody-S07-4C9-Western-Blot-NBP3-20014-img0001.jpg "Western Blot: TORC3/CRTC3 Antibody (S07-4C9) [NBP3-20014] - Western blot detection of TORC3/CRTC3 in K562, Rat Brain, C6, 3T3, Hela cell lysates using NBP3-20014 (1:1000 diluted). Predicted band size: 67kDa. Observed band size: 78kDa.")
![Immunocytochemistry/Immunofluorescence: TORC3/CRTC3 Antibody [NBP2-56127] - Staining of human cell line SiHa shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/TORC3-CRTC3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56127-img0001.jpg "Immunocytochemistry/Immunofluorescence: TORC3/CRTC3 Antibody [NBP2-56127] - Staining of human cell line SiHa shows localization to nucleoplasm & cytosol.")
![Immunohistochemistry:[NBP3-29293]-Detection of human TORC3 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-TORC3.](http://images.novusbio.com/fullsize/nbp3-29293_rabbit-torc3-crtc3-pab-23120251225694.jpg "Immunohistochemistry:[NBP3-29293]-Detection of human TORC3 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-TORC3.")
![Immunohistochemistry: TORC3/CRTC3 Antibody (10G3) [NBP3-26644] - Image of TORC3/CRTC3 Antibody (10G3) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26644_rabbit-torc3-crtc3-mab-10g3-262202414471556.jpg "Immunohistochemistry: TORC3/CRTC3 Antibody (10G3) [NBP3-26644] - Image of TORC3/CRTC3 Antibody (10G3) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.")
