TORC3/CRTC3 Antibody (2069D) [Unconjugated]

TORC3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human TORC3 Monoclonal Antibody (Catalog # MAB9455) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 2069D
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

TORC3/CRTC3 Antibody (2069D) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: E. coli-derived recombinant human TORC3
  Ser425-Asn574
  Accession # Q6UUV7
• Specificity: Detects human TORC3 in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 1-10 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TORC3/CRTC3 Antibody (2069D) [Unconjugated]

• CREB regulated transcription coactivator 3
• CRTC3
• FLJ21868
• TORC3
• TORC-3
• TORC3CREB-regulated transcription coactivator 3
• Transducer of CREB protein 3
• transducer of regulated cAMP response element-binding protein (CREB) 3
• Transducer of regulated cAMP response element-binding protein 3
• transducer of regulated CREB protein 3

Background

CREB-Regulated Transcription Coactivator 3 (CRTC3), also known as TORC3, a transcriptional coactivator for CREB1, acts as a coactivator in the SIK/TORC signaling pathway. Dephosphorylated CRTC3 increases the interaction of CREB1 cAMP response element (CRE) sites. CRTC3 activity is important in regulating the expression of genes involved in cellular energy metabolism. Its binding to the HTLV-1 protein Tax enhances HTLV-1 transcription, whereas its binding to cellular Bcl3 inhibits HTLV-1 transcription. CRTC3 polymorphism have been associated with obesity and hypertriglyceridemia. 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q6UUV7()