TOM70 Antibody

Images

 
Western Blot: TOM70 Antibody [NB110-58347] - Detection of TOM70 in MCF7 mitochondrial lysate
Immunocytochemistry/ Immunofluorescence: TOM70 Antibody [NB110-58347] - TOM70 antibody was tested in MCF7 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
Simple Western: TOM70 Antibody [NB110-58347] - Simple Western lane view shows a specific band for TOM70 in 0.5 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

TOM70 Antibody Summary

Immunogen
A synthetic peptide made to a region within residues 1-100 of the human TOM70 protein. [Swiss-Prot# O94826]
Localization
Mitochondrial; multi-pass membrane.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TOMM70A
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Simple Western 1:50
  • Immunocytochemistry/Immunofluorescence 1:100
Application Notes
This TOM70 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen at approx. 67 kDa. In ICC/IF mitochondrial staining was observed in MCF7 cells.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB110-58347 in the following applications:

Reactivity Notes

Human, mouse, and rat.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A purified

Alternate Names for TOM70 Antibody

  • FLJ90470
  • KIAA0719
  • mitochondrial import receptor subunit TOM70
  • Mitochondrial precursor proteins import receptor
  • TOM70
  • Translocase of outer membrane 70 kDa subunit
  • translocase of outer mitochondrial membrane 70 (yeast) homolog A
  • translocase of outer mitochondrial membrane 70 homolog A (S. cerevisiae)
  • translocase of outer mitochondrial membrane 70 homolog A (yeast)

Background

Most of mitochondrial proteins are synthesized by cytosolic ribosomes followed by import into mitochondria post-translationally with the help of cytosolic chaperones as well as mitochondrial translocators (import components). Translocase of the outer membrane (TOM) complex (TOMM5, TOMM6, TOMM7, TOMM20, TOMM22, TOMM40 and TOMM70) is the proteinaceous machinery that contains surface receptors for pre-protein recognition and serve as main entry gateway into mitochondria. Mitochondrial targeting requires different cytosolic factors, in particular the molecular chaperones (Hsc70/Hsp70 and Hsp90) whose activity occur in coordinated cycles of ATP hydrolysis/substrate binding and are regulated by a number of co-chaperone proteins including TOM70, a member of TPR (tetratricopeptide repeat) co-chaperone family. TOM70 contains a conserved TPR clamp domain for interaction with Hsc70 and Hsp90, and such interaction is essential for import process initiation. TOMM70 is a single-pass membrane protein localized in mitochondrion outer membrane and is ubiquitously expressed in various tissues where it acts as a receptor that accelerates the import of all mitochondrial precursor proteins. In addition to importing inner membrane carrier proteins, Tom70 is involved in targeting Mcl-1 to outer membrane via an internal EELD sequence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TOM70 Antibody (NB110-58347)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TOM70 Antibody (NB110-58347). (Showing 1 - 1 of 1 FAQs).

  1. Our customer would like to know if this kit can work in Saccharomices samples in WB?
    • The antibody you are referring to, NB110-58347, has been validated for use in Human, Mouse and Rat for Western Blot. Saccharomyces is not one of the listed species that we guarantee this antibody to work in. Should you customer be interested in testing this antibody, please refer them to our Innovators Reward Program.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TOMM70A