SAMM50 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: SAMM50 Antibody [NBP1-84509] - Staining of human cell line A-431 shows localization to mitochondria. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509] - Staining of human parathyroid gland shows very strong granular cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509] - Staining of human small intestine shows strong cytoplasmic granular positivity in glandular cells.

Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509] - Staining of human kidney shows very strong cytoplasmic granular positivity in cells in tubules.

Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509] - Staining of human pancreas shows moderate cytoplasmic granular positivity in exocrine glandular cells.

Simple Western: SAMM50 Antibody [NBP1-84509] - Simple Western lane view shows a specific band for SAMM50 in 0.2 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: SAMM50 Antibody [NBP1-84509] - Electropherogram image(s) of corresponding Simple Western lane view. SAMM50 antibody was used at 1:4 dilution on RT-4 and U-251MG lysate(s).

Staining of human testis shows strong cytoplasmic granular positivity in Leydig cells.

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Analysis in human cell line HL-60.

Analysis in human kidney and pancreas tissues using HPA034537 antibody. Corresponding SAMM50 RNA-seq data are presented for the same tissues.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SAMM50 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SAMM50 Antibody - BSA Free (NBP1-84509) is a polyclonal antibody validated for use in IHC, WB, ICC/IF, Simple Western and IP. Anti-SAMM50 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: HARSLEPLPSSGPDFGGLGEEAEFVEVEPEAKQEILENKDVVVQHVHFDGLGRTKDDIIICEIGDVFKAKNLIEVMRKSHEAREKLLRLGIFRQVDVLID
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SAMM50
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Immunoprecipitation Reported in scientific literature (PMID:26308446).
  • Simple Western 1:4
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. IHC-Paraffin HIER pH6 retrieval is recommended.
  See Simple Western Antibody Database for Simple Western validation: Tested in RT-4 and U-251MG, separated by Size, antibody dilution of 1:4, apparent MW was 56 kDa
• Control Peptide:

  SAMM50 Protein (NBP1-84509PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for SAMM50 Antibody - BSA Free

• CGI-51
• FLJ42905
• FLJ99036
• OMP85
• SAM50
• sorting and assembly machinery 50kDa
• sorting and assembly machinery component 50 homolog (S. cerevisiae)
• sorting and assembly machinery component 50 homolog
• TOB55
• Transformation-related gene 3 protein
• transformation-related protein 3
• TRG-3FLJ35825
• YNL026W

Background

SAMM50 is a component of the sorting and assembly machinery (SAM) complex of the outer mitochondrial membrane. The SAMcomplex has a role in integrating beta-barrel proteins into the outer mitochondrial membrane (Humphries et al., 2005(PubMed 15644312)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SAMM50 Antibody (NBP1-84509)(1)

Publication using NBP1-84509

• Takamitsu E, Otsuka M, Haebara T et al. Identification of Human N-Myristoylated Proteins from Human Complementary DNA Resources by Cell-Free and Cellular Metabolic Labeling Analyses. PLoS One 2015-01-01 [PMID: 26308446] (IP, ICC/IF, Human)

Bioinformatics

• Gene Symbol: SAMM50