SAMM50 Antibody

Images

 
Western Blot: SAMM50 Antibody [NBP1-84509] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: SAMM50 Antibody [NBP1-84509] - Staining of human cell line A-431 shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509] - Staining of human parathyroid gland shows very strong granular cytoplasmic positivity in glandular cells.
Western Blot: SAMM50 Antibody [NBP1-84509] - Analysis in human cell line HL-60.
Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509] - Staining of human parathyroid gland shows high expression.
Orthogonal Strategies: Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509] - Analysis in human kidney and pancreas tissues. Corresponding SAMM50 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509] - Staining of human small intestine shows strong cytoplasmic granular positivity in glandular cells.
Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509] - Staining of human testis shows strong cytoplasmic granular positivity in Leydig cells.
Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509] - Staining of human kidney shows very strong cytoplasmic granular positivity in cells in tubules.
Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509] - Staining of human pancreas shows moderate cytoplasmic granular positivity in exocrine glandular cells.
Simple Western: SAMM50 Antibody [NBP1-84509] - Simple Western lane view shows a specific band for SAMM50 in 0.2 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing conditions ...read more
Simple Western: SAMM50 Antibody [NBP1-84509] - Electropherogram image(s) of corresponding Simple Western lane view. SAMM50 antibody was used at 1:4 dilution on RT-4 and U-251MG lysate(s).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

SAMM50 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: HARSLEPLPSSGPDFGGLGEEAEFVEVEPEAKQEILENKDVVVQHVHFDGLGRTKDDIIICEIGDVFKAKNLIEVMRKSHEAREKLLRLGIFRQVDVLID
Specificity
Specificity of human SAMM50 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SAMM50
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.04-0.4 ug/ml
  • Simple Western 1:4
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Immunoprecipitation
Application Notes
IP, ICC/IF reported in the literature (PMID:26308446). ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. IHC-Paraffin HIER pH6 retrieval is recommended.
Control Peptide
SAMM50 Protein (NBP1-84509PEP)
Publications
Read Publication using
NBP1-84509 in the following applications:

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 26308446).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SAMM50 Antibody

  • CGI-51
  • FLJ42905
  • FLJ99036
  • OMP85
  • SAM50
  • sorting and assembly machinery 50kDa
  • sorting and assembly machinery component 50 homolog (S. cerevisiae)
  • sorting and assembly machinery component 50 homolog
  • TOB55
  • Transformation-related gene 3 protein
  • transformation-related protein 3
  • TRG-3FLJ35825
  • YNL026W

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-84509
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Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IP

Publications for SAMM50 Antibody (NBP1-84509)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, IP.


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ICC/IF
(1)
IP
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol SAMM50