Monkey TNF RI/TNFRSF1A ELISA Kit (Colorimetric) Summary
Description |
The monkey sTNF-R I ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of monkey sTNF-R I |
Specificity |
The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a monkey TNF-R (60 kDa) positive serum. There was no crossreactivity detected. |
Standard Curve Range |
0.09 - 6.0 U/mL |
Sensitivity |
0.06 U/mL |
Assay Type |
Sandwich ELISA |
Inter-Assay |
CV% < 9.0% |
Intra-Assay |
CV% < 2.0% |
Spike Recovery |
The overall mean recovery was 93%. Please see protocol for more specific Spike Recovery details. |
Sample Volume |
10 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
TNFRSF1A |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Adhesive Plate Covers
- Aluminium pouch(es) with a Microwell Plate coated with monoclonal Antibody (murine) to monkey sTNF-R I, HRP-Conjugate (anti-monkey sTNF-R I monoclonal (murine) antibody) and Assay Buffer, lyophilized
- Aluminium pouch(es) with a monkey sTNF-R I Standard curve (coloured)
- Assay Buffer Concentrate 20x (Use when an external predilution of the samples is needed)
- Stop Solution (1M Phosphoric acid)
- Substrate Solution (tetramethyl-benzidine)
- Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)
|
Alternate Names for Monkey TNF RI/TNFRSF1A ELISA Kit (Colorimetric)
Background
The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein is one of the major receptors for the tumor necrosis factor-alpha. This receptor can activate NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor. Germline mutations of the extracellular domains of this receptor were found to be associated with the autosomal dominant periodic fever syndrome. The impaired receptor clearance is thought to be a mechanism of the disease. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for TNF RI/TNFRSF1A ELISA Kit (NBP1-92722). (Showing 1 - 1 of 1 FAQ).
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wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
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