Recombinant Human TNF RI/TNFRSF1A Protein

SDS-Page: Recombinant Human TNF RI/TNFRSF1A Protein [NBP2-35274]

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human TNF RI/TNFRSF1A Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 190 amino acids corresponding to TNF RI/TNFRSF1A Source: E. coli

  Uniprot ID: P19438

  Amino Acid Sequence: IYPSGVIGLV PHLGDREKRD SVCPQGKYIH PQNNSICCTK CHKGTYLYND CPGPGQDTDC RECESGSFTA SENHLRHCLS CSKCRKEMGQ VEISSCTVDR DTVCGCRKNQ YRHYWSENLF QCFNCSLCLN GTVHLSCQEK QNTVCTCHAG FFLRENECVS CSNCKKSLEC TKLCLPQIEN VKGTEDSGTT

• Details of Functionality: TNF RI/TNFRSF1A protein is fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the TNF-alpha mediated cytotoxicity in the L-929 cells is less than 0.05 ug/ml, corresponding to a specific activity of > 2 x 10^4 IU/mg in the presence of 0.25 ng/mL of rHuTNF-alpha.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: TNFRSF1A
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 0.1 EU/ug of TNF RI/TNFRSF1A as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 21.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Human TNF RI/TNFRSF1A Protein

• CD120a antigen
• CD120a
• FPF
• p55
• p55-R
• p60
• sTNF RI
• sTNF RI/TNFRSF1A
• TNF RI
• TNFARMGC19588
• TNF-R
• TNF-R1
• TNFR1TBP1
• TNFR55
• TNF-R55
• TNFR60
• TNFRI
• TNF-RI
• TNF-R-I
• TNFR-I
• TNFRSF1A
• tumor necrosis factor binding protein 1
• Tumor necrosis factor receptor 1
• tumor necrosis factor receptor 1A isoform beta
• tumor necrosis factor receptor superfamily member 1A
• tumor necrosis factor receptor superfamily, member 1A
• tumor necrosis factor receptor type 1
• Tumor necrosis factor receptor type I
• tumor necrosis factor-alpha receptor

Background

TNF Receptor I (TNF RI), also named as TNFR-I, TNF R1, TNFRSF1A, p55 and p60, belongs to the tumor necrosis factor receptor superfamily, and it is encoded by the TNFRSF1A gene in human. TNF Receptor I (TNF RI)is a universally expressed protein and is thought to form homotrimers after ligand binding. TNF Receptor I (TNF RI) is one of the major receptors for the TNF-alpha. This receptor can activate the transcription factor NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Mature human soluble TNF RI shares 68 % ~ 73 % amino acid sequence identity with murine, rat, bovine and porcine soluble TNF RI.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: TNFRSF1A
• Uniprot:
  • P19438()
  • P19438()
  • P19438()