TNF RI/TNFRSF1A Antibody [Biotin]

• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

TNF RI/TNFRSF1A Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human sTNF RI
• Specificity: Detects human TNF RI in ELISAs and Western blots. In sandwich immunoassays, less than 0.02% cross-reactivity with recombinant human (rh) TNF‑ alpha , recombinant mouse (rm) TNF‑ alpha , recombinant porcine TNF‑ alpha , recombinant rat TNF‑ alpha , rhTNF‑ beta , rmsTNF RI, rhsTNF RII, and rmsTNF RII is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TNFRSF1A
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Application Notes: ELISA Capture: Human TNF RI/TNFRSF1A Antibody (Catalog # MAB625)
  ELISA Detection: Human TNF RI/TNFRSF1A Biotinylated Antibody (Catalog # BAF225)
  Standard: Recombinant Human TNF RI/TNFRSF1A (Catalog # 636-R1)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

TNF receptor 1 (TNF RI; also called TNF R-p55/p60 and TNFRSF1A) is a type I transmembrane protein member of the TNF receptor superfamily member, designated TNFRSF1A (1, 2). Both TNF RI and TNF RII (TNFRSF1B) are widely expressed and contain four TNF-  alpha trimer-binding cysteine-rich domains (CRD) in their extracellular domains (ECD). However, TNF RI is thought to mediate most of the cellular effects of TNF-alpha (3). It is essential for proper development of lymph node germinal centers and Peyer’s patches, and for combating intracellular pathogens such as Listeria (1 - 3). TNF RI is also a receptor for TNF-beta /TNFSF1B (lymphotoxin-alpha ) (4). TNF RI is present on the cell surface as a trimer of 55 kDa subunits (4, 5). TNF-alpha induces sequestering of TNF RI in lipid rafts, where it activates NF kappa B and is cleaved by ADAM-17/TACE (9, 10). Release of the 28 - 34 kDa TNF RI ECD also occurs constitutively and in response to products of pathogens such as LPS, CpG DNA or S. aureus protein A (1, 6 - 8). Full-length TNF RI may also be released in exosome-like vesicles (11). Release helps to resolve inflammatory reactions, since it down-regulates cell surface TNF RI and provides soluble TNF RI to bind TNF-alpha (6, 12, 13). Exclusion from lipid rafts causes endocytosis of TNF RI complexes and induces apoptosis (1). Human TNF RI is a 455 amino acid (aa) protein that contains a 21 aa signal sequence, a 190 aa ECD with a PLAD domain(5) that mediates constitutive trimer formation, followed by the four CRD, a 23 aa transmembrane domain, and a 221 aa cytoplasmic sequence that contains a neutral sphingomyelinase activation domain and a death domain (15). The ECD of human TNF RI shows 80%, 80%, 73%, 69% and 70% aa identity with dog, cat, pig, rat and mouse TNF RI, respectively; it shows 23% aa identity with the ECD of TNF RII.

1. Pfeffer, K. (2003) Cytokine Growth Factor Rev. 14:185.
2. Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1.
3. Peschon, J.J. et al. (1998) J. Immunol. 160:943.
4. Banner, D.W et al. (1993) Cell 73: 431.
5. Chan, F.K. et al. (2000) Science 288:2351.
6. Xanthoulea, S. et al. (2004) J. Exp. Med. 200:367.
7. Jin, L. et al. (2000) J. Immunol. 165:5153.
8. Gomez, M.I. et al. (2006) J. Biol. Chem. 281:20190.
9. Legler, D.F. et al. (2003) Immunity 18:655.
10. Tellier, E. et al. (2006) Exp. Cell Res. 312:3969.
11. Islam, A. et al. (2006) J. Biol. Chem. 281:6860.
12. Garton, K.J. et al. (2006) J. Leukoc. Biol. 79:1105.
13. McDermott, M.F. et al. (1999) Cell 97:133.
14. Schneider-Brachert, W. et al. (2004) Immunity 21:415.
15. Schall, T.J. et al. (1990) Cell 61:361.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TNFRSF1A