TNF RI/TNFRSF1A Antibody

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Western blot shows lysates of L‑929 mouse fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse TNF RI/TNFRSF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-425-PB) followed ...read more
L‑929 mouse fibroblast cell line was stained with Goat Anti‑Mouse TNF RI/TNFRSF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-425-PB, filled histo­gram) or control antibody (Catalog # AB-108-C, ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready, Func
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

TNF RI/TNFRSF1A Antibody Summary

Immunogen
E. coli-derived recombinant mouse TNF RI
Ile22-Ala212
Accession # P25118
Specificity
Detects mouse TNF RI/TNFRSF1A in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing conditions), less than 5% cross-reactivity with recombinant human TNF RI is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TNFRSF1A
Purity
Immunogen affinity purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Agonist Activity
  • CyTOF-ready
Publications
Read Publications using
AF-425-PB in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TNF RI/TNFRSF1A Antibody

  • CD120a antigen
  • CD120a
  • FPF
  • p55
  • p55-R
  • p60
  • TNF RI
  • TNFARMGC19588
  • TNF-R
  • TNF-R1
  • TNFR1TBP1
  • TNFR55
  • TNF-R55
  • TNFR60
  • TNFRI
  • TNF-RI
  • TNF-R-I
  • TNFR-I
  • TNFRSF1A
  • tumor necrosis factor binding protein 1
  • Tumor necrosis factor receptor 1
  • tumor necrosis factor receptor 1A isoform beta
  • tumor necrosis factor receptor superfamily member 1A
  • tumor necrosis factor receptor superfamily, member 1A
  • tumor necrosis factor receptor type 1
  • Tumor necrosis factor receptor type I
  • tumor necrosis factor-alpha receptor

Background

TNF receptor 1 (TNF RI; also called TNF R‑p55/p60, TNFRSF1A and CD120a) is a type I transmembrane protein that belongs to the TNF receptor superfamily (1, 2). TNF RI is widely expressed and is present on the cell surface as a trimer of 55 kDa subunits. It serves as a receptor for both TNF‑ alpha and TNF‑ beta /lymphotoxin. Each subunit contains four TNF‑ alpha trimer‑binding cysteine‑rich domains (CRD) in its extracellular domain (ECD) (1‑6). TNF‑ alpha binding to TNF R1 induces the sequestration of TNFRI in lipid rafts, where it activates NF kappa B and is cleaved by ADAM‑17/TACE (7, 8). Release of the 28‑34 kDa TNF RI ECD occurs constitutively, and in response to products of pathogens such as LPS, CpG DNA or S. aureus protein A (1, 7‑12). Full‑length TNF RI may also be released in exosome‑like vesicles (12). Such release helps to resolve inflammatory reactions as it down‑regulates cell surface TNF RI and provides soluble TNF RI to bind TNF‑ alpha (6, 13, 14). Exclusion from lipid rafts causes endocytosis of TNF RI complexes and induces apoptosis (7, 15). Although there is a second receptor for TNF‑ alpha (TNF R2), TNF RI is thought to mediate most of the cellular effects of TNF‑ alpha (3). TNF R1 is essential for proper development of lymph node germinal centers and Peyer’s patches, and for combating intracellular pathogens such as Listeria monocytogenes (1‑3). Mouse TNF RI is a 454 amino acid (aa) protein that contains a 21 aa signal sequence and a 191 aa ECD with a PLAD domain (6). This mediates constitutive trimer formation. The PLAD domain is followed by four CRDs, a 23 aa transmembrane domain, and a 219 aa cytoplasmic sequence that contains a neutral sphingomyelinase activation domain and a death domain (16). The ECD of mouse TNF RI shows 67%, 70%, 64%, 70% and 88% aa identity with canine, feline, procine, human and rat TNF RI, respectively; and it shows 23% aa identity with the ECD of TNF RII.

  1. Pfeffer, K. (2003) Cytokine Growth Factor Rev. 14:185.
  2. Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1.
  3. Peschon, J.J. et al. (1998) J. Immunol. 160:943.
  4. Banner, D.W et al. (1993) Cell 73:431.
  5. Medvedev, A.E. et al. (1996) J. Biol. Chem. 271:9778.
  6. Chan, F.K. et al. (2000) Science 288:2351.
  7. Legler, D.F. et al. (2003) Immunity 18:655.
  8. Tellier, E. et al. (2006) Exp. Cell Res. 312:3969.
  9. Xanthoulea, S. et al. (2004) J. Exp. Med. 200:367.
  10. Jin, L. et al. (2000) J. Immunol. 165:5153.
  11. Gomez, M.I. et al. (2006) J. Biol. Chem. 281:20190.
  12. Islam, A. et al. (2006) J. Biol. Chem. 281:6860.
  13. Garton, K.J. et al. (2006) J. Leukoc. Biol. 79:1105.
  14. McDermott, M.F. et al. (1999) Cell 97:133.
  15. Schneider-Brachert, W. et al. (2004) Immunity 21:415.
  16. Lewis, M. et al. (1991) Proc. Natl. Acad. Sci. USA 88:2830.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TNF RI/TNFRSF1A Antibody (AF-425-PB)(13)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 9 applications: Complex Application, ELISA Development, Flow Cytometry, ICC, IHC, IHC-Fr, IP Western, Immunoprecipitation, Western Blot.


Filter By Application
Complex Application
(1)
ELISA Development
(1)
Flow Cytometry
(3)
ICC
(1)
IHC
(1)
IHC-Fr
(1)
IP Western
(1)
Immunoprecipitation
(2)
Western Blot
(2)
All Applications
Filter By Species
Mouse
(12)
Rat
(1)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using AF-425-PB Applications Species
A Polykratis, A Martens, RO Eren, Y Shirasaki, M Yamagishi, Y Yamaguchi, S Uemura, M Miura, B Holzmann, G Kollias, M Armaka, G van Loo, M Pasparakis A20 prevents inflammasome-dependent arthritis by inhibiting macrophage necroptosis through its ZnF7 ubiquitin-binding domain Nat. Cell Biol., 2019;0(0):. 2019 [PMID: 31086261] (IP Western, Mouse) IP Western Mouse
H Meng, Z Liu, X Li, H Wang, T Jin, G Wu, B Shan, DE Christoffe, C Qi, Q Yu, Y Li, J Yuan Death-domain dimerization-mediated activation of RIPK1 controls necroptosis and RIPK1-dependent apoptosis Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):. 2018 [PMID: 29440439] (Western Blot, Mouse) Western Blot Mouse
S Martens, M Jeong, W Tonnus, F Feldmann, S Hofmans, V Goossens, N Takahashi, JH Bräsen, EW Lee, P Van der Ve, J Joossens, K Augustyns, S Fulda, A Linkermann, J Song, P Vandenabee Sorafenib tosylate inhibits directly necrosome complex formation and protects in mouse models of inflammation and tissue injury Cell Death Dis, 2017;8(6):e2904. 2017 [PMID: 28661484] (Immunoprecipitation, Mouse) Immunoprecipitation Mouse
S Arokiasamy, C Zakian, J Dilliway, W Wang, S Nourshargh, MB Voisin Endogenous TNF? orchestrates the trafficking of neutrophils into and within lymphatic vessels during acute inflammation Sci Rep, 2017;7(0):44189. 2017 [PMID: 28287124] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Li L, Chen Y, Doan J, Murray J, Molkentin J, Liu Q Transforming growth factor beta-activated kinase 1 signaling pathway critically regulates myocardial survival and remodeling. Circulation, 2014;130(24):2162-72. 2014 [PMID: 25278099] (Western Blot, Mouse) Western Blot Mouse
Haga E, Endo Y, Haruta M, Koba C, Matsumura K, Takamatsu K, Ikeda T, Nishimura Y, Senju S Therapy of peritoneally disseminated colon cancer by TAP-deficient embryonic stem cell-derived macrophages in allogeneic recipients. J Immunol, 2014;193(4):2024-33. 2014 [PMID: 25031460] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Freeman B, Meyer C, Slifka M Anti-inflammatory cytokines directly inhibit innate but not adaptive CD8+ T cell functions. J Virol, 2014;88(13):7474-84. 2014 [PMID: 24741101] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Proebstl D, Voisin MB, Woodfin A, Whiteford J, D'Acquisto F, Jones GE, Rowe D, Nourshargh S Pericytes support neutrophil subendothelial cell crawling and breaching of venular walls in vivo. J. Exp. Med., 2012;209(6):1219-34. 2012 [PMID: 22615129] (IHC, Mouse) IHC Mouse
Baluk P, Yao LC, Feng J, Romano T, Jung SS, Schreiter JL, Yan L, Shealy DJ, McDonald DM TNF-alpha drives remodeling of blood vessels and lymphatics in sustained airway inflammation in mice. J. Clin. Invest., 2009;119(10):2954-64. 2009 [PMID: 19759514] (IHC-Fr, Mouse) IHC-Fr Mouse
Saperstein S, Chen L, Oakes D, Pryhuber G, Finkelstein J IL-1beta augments TNF-alpha-mediated inflammatory responses from lung epithelial cells. J Interferon Cytokine Res, 0;29(5):273-84. 0 [PMID: 19231998] (Complex Application, Mouse) Complex Application Mouse
Show All 13 Publications.

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Biotin BAF425

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Bioinformatics

Gene Symbol TNFRSF1A
Uniprot