TNF RI/TNFRSF1A Antibody (16803) [Unconjugated]

Images

 
Recombinant Human TNF RI/TNFRSF1A (Catalog # 372-R1) inhibits Recombinant Human TNF‑ alpha (Catalog #210-TA) induced cytotoxicity in the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as ...read more
Human peripheral blood monocytes were stained with Mouse Anti-Human TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB225, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, B/N, CyTOF-ready
Clone
16803
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

TNF RI/TNFRSF1A Antibody (16803) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human TNF RI/TNFRSF1A
Accession # NP_001056
Specificity
Detects human TNF RI in Western blots. In Western blots, does not cross-react with recombinant human (rh) TNF RII, rmTNF RI, or rmTNF RII.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TNFRSF1A
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready
  • Neutralization 0.008-0.04 ug/mL
Publications
Read Publications using
MAB225 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TNF RI/TNFRSF1A Antibody (16803) [Unconjugated]

  • CD120a antigen
  • CD120a
  • FPF
  • p55
  • p55-R
  • p60
  • TNF RI
  • TNFARMGC19588
  • TNF-R
  • TNF-R1
  • TNFR1TBP1
  • TNFR55
  • TNF-R55
  • TNFR60
  • TNFRI
  • TNF-RI
  • TNF-R-I
  • TNFR-I
  • TNFRSF1A
  • tumor necrosis factor binding protein 1
  • Tumor necrosis factor receptor 1
  • tumor necrosis factor receptor 1A isoform beta
  • tumor necrosis factor receptor superfamily member 1A
  • tumor necrosis factor receptor superfamily, member 1A
  • tumor necrosis factor receptor type 1
  • Tumor necrosis factor receptor type I
  • tumor necrosis factor-alpha receptor

Background

TNF RI occurs both in membrane bound and soluble forms and functions as a receptor for TNF-alpha and TNF-beta. In the superfamily nomenclature, it is designated TNFRSF1A.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NBP2-79843
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, CyTOF-ready
H00001523-M01
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP, S-ELISA
MAB224
Species: Hu
Applications: Flow, IHC, CyTOF-ready, Neut
MAB3468
Species: Hu
Applications: WB, ICC
H00003059-M02
Species: Hu
Applications: WB, ELISA, IHC, IHC-P, PLA, RNAi, S-ELISA
NBP1-18910
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, IP
AF1138
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready, Neut
7270-IL/CF
Species: Hu
NB100-56583
Species: Hu, Rt, Rb
Applications: WB, Flow, IHC, IHC-P, KO
NB100-56173
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP

Publications for TNF RI/TNFRSF1A Antibody (MAB225)(23)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: Cell Culture, Flow Cytometry, IHC, Neutralization, Western Blot.


Filter By Application
Cell Culture
(1)
Flow Cytometry
(10)
IHC
(1)
Neutralization
(10)
Western Blot
(1)
All Applications
Filter By Species
Human
(20)
Mouse
(2)
Rat
(1)
All Species
Showing Publications 1 - 10 of 23. Show All 23 Publications.
Publications using MAB225 Applications Species
Q Zhou, X Wu, X Wang, Z Yu, T Pan, Z Li, X Chang, Z Jin, J Li, Z Zhu, B Liu, L Su The reciprocal interaction between tumor cells and activated fibroblasts mediated by TNF-alpha/IL-33/ST2L signaling promotes gastric cancer metastasis Oncogene, 2019;0(0):. 2019 [PMID: 31659258] (Cell Culture, Human) Cell Culture Human
F Minshawi, MRH White, W Muller, N Humphreys, D Jackson, BJ Campbell, A Adamson, S Papoutsopo Human TNF-Luc reporter mouse: A new model to quantify inflammatory responses Sci Rep, 2019;9(1):193. 2019 [PMID: 30655563] (Neutralization, Human) Neutralization Human
M Monguió-To, M Franquesa, MR Sarrias, FE Borràs Low doses of LPS exacerbate the inflammatory response and trigger death on TLR3-primed human monocytes Cell Death Dis, 2018;9(5):499. 2018 [PMID: 29717111] (Neutralization, Human) Neutralization Human
AT Holdbrooks, CM Britain, SL Bellis ST6Gal-I sialyltransferase promotes tumor necrosis factor (TNF)-mediated cancer cell survival via sialylation of the TNF receptor 1 (TNFR1) death receptor J. Biol. Chem., 2017;0(0):. 2017 [PMID: 29233887] (Neutralization, Human) Neutralization Human
A Chakrabort, KA Dorsett, HQ Trummell, ES Yang, PG Oliver, JA Bonner, DJ Buchsbaum, SL Bellis ST6Gal-I sialyltransferase promotes chemoresistance in pancreatic ductal adenocarcinoma by abrogating gemcitabine-mediated DNA damage J. Biol. Chem., 2017;0(0):. 2017 [PMID: 29191829] (Neutralization, Human) Neutralization Human
SL Ross, M Sherman, PL McElroy, JA Lofgren, G Moody, PA Baeuerle, A Coxon, T Arvedson Bispecific T cell engager (BiTE�) antibody constructs can mediate bystander tumor cell killing PLoS ONE, 2017;12(8):e0183390. 2017 [PMID: 28837681] (Neutralization, Human) Neutralization Human
TNF? promotes CAR-dependent migration of leukocytes across epithelial monolayers Sci Rep, 2016;6(0):26321. 2016 [PMID: 27193388] (Neutralization, Human) Neutralization Human
TNF? promotes CAR-dependent migration of leukocytes across epithelial monolayers Sci Rep, 2016;6(0):26321. 2016 [PMID: 27193388] (Neutralization, Human) Neutralization Human
Sonoda Y, Gohda T, Suzuki Y, Omote K, Ishizaka M, Matsuoka J, Tomino Y Circulating TNF receptors 1 and 2 are associated with the severity of renal interstitial fibrosis in IgA nephropathy. PLoS ONE, 2015;10(4):e0122212. 2015 [PMID: 25860248] (IHC, Human) IHC Human
Mizrahi K, Ash S, Peled T, Yaniv I, Stein J, Askenasy N Negative selection by apoptosis enriches progenitors in naive and expanded human umbilical cord blood grafts. Bone Marrow Transplant, 0;49(7):942-9. 0 [PMID: 24777188] (Flow Cytometry, Human) Flow Cytometry Human
Show All 23 Publications.

Reviews for TNF RI/TNFRSF1A Antibody (MAB225) (0)

There are no reviews for TNF RI/TNFRSF1A Antibody (MAB225). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TNF RI/TNFRSF1A Antibody (MAB225) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional TNF RI/TNFRSF1A Products

Bioinformatics Tool for TNF RI/TNFRSF1A Antibody (MAB225)

Discover related pathways, diseases and genes to TNF RI/TNFRSF1A Antibody (MAB225). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TNF RI/TNFRSF1A Antibody (MAB225)

Discover more about diseases related to TNF RI/TNFRSF1A Antibody (MAB225).
 

Pathways for TNF RI/TNFRSF1A Antibody (MAB225)

View related products by pathway.

PTMs for TNF RI/TNFRSF1A Antibody (MAB225)

Learn more about PTMs related to TNF RI/TNFRSF1A Antibody (MAB225).

Blogs on TNF RI/TNFRSF1A

There are no specific blogs for TNF RI/TNFRSF1A, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our TNF RI/TNFRSF1A Antibody (16803) [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol TNFRSF1A
Uniprot