TNF RI/TNFRSF1A Antibody (16803) [Phycoerythrin]

Human peripheral blood monocytes were stained with Mouse Anti-Human TNF RI/TNFRSF1A PE-conjugated Monoclonal Antibody (Catalog # FAB225P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 16803
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

TNF RI/TNFRSF1A Antibody (16803) [Phycoerythrin] Summary

• Immunogen: E. coli-derived recombinant human TNF RI/TNFRSF1A
  Accession # NP_001056
• Specificity: Detects human TNF RI in Western blots. In Western blots, does not cross-react with recombinant human TNF RII, recombinant mouse (rm) TNF RI, or rmTNF RII.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNFRSF1A
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TNF RI/TNFRSF1A Antibody (16803) [Phycoerythrin]

• CD120a antigen
• CD120a
• FPF
• p55
• p55-R
• p60
• TNF RI
• TNFARMGC19588
• TNF-R
• TNF-R1
• TNFR1TBP1
• TNFR55
• TNF-R55
• TNFR60
• TNFRI
• TNF-RI
• TNF-R-I
• TNFR-I
• TNFRSF1A
• tumor necrosis factor binding protein 1
• Tumor necrosis factor receptor 1
• tumor necrosis factor receptor 1A isoform beta
• tumor necrosis factor receptor superfamily member 1A
• tumor necrosis factor receptor superfamily, member 1A
• tumor necrosis factor receptor type 1
• Tumor necrosis factor receptor type I
• tumor necrosis factor-alpha receptor

Background

TNF RI occurs both in membrane bound and soluble forms and functions as a receptor for TNF-alpha and TNF-beta . In the superfamily nomenclature, it is designated TNFRSF1A.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TNF RI/TNFRSF1A Antibody (FAB225P)(25)

Publications using FAB225P

• Alshevskaya, AA;Lopatnikova, JA;Zhukova, JV;Perik-Zavodskaia, OY;Alrhmoun, S;Obleukhova, IA;Matveeva, AK;Savenkova, DA;Imatdinov, IR;Yudkin, DV;Sennikov, SV; TNFR1 Absence Is Not Crucial for Different Types of Cell Reaction to TNF: A Study of the TNFR1-Knockout Cell Model Epigenomes 2024-04-03 [PMID: 38651368] (Flow Cytometry, Human)
• Perik-Zavodskaia, O;Alrhmoun, S;Perik-Zavodskii, R;Zhukova, J;Lopatnikova, J;Volynets, M;Alshevskaya, A;Sennikov, S; Knockouts of TNFRSF1A and TNFRSF1B Genes in K562 Cell Line Lead to Diverse Long-Lasting Responses to TNF-? International journal of molecular sciences 2023-12-06 [PMID: 38138998] (Flow Cytometry, Human)
• A Alshevskay, J Lopatnikov, J Zhukova, O Chumasova, N Shkaruba, A Sizikov, I Evsegneeva, D Demina, V Nepomniash, A Karaulov, S Sennikov The Influence of Severity and Disease Duration on TNF Receptors' Redistribution in Asthma and Rheumatoid Arthritis Cells, 2022-12-20;12(1):. 2022-12-20 [PMID: 36611799] (Flow Cytometry, Human)
• J. Agustin Cruz, Chaitanya S. Mokashi, Gabriel J. Kowalczyk, Yue Guo, Qiuhong Zhang, Sanjana Gupta et al. A variable-gain stochastic pooling motif mediates information transfer from receptor assemblies into NF-kappa B Science Advances 2021-07-23 [PMID: 34301608]
• Twomey JD, Zhang B. Circulating Tumor Cells Develop Resistance to TRAIL-Induced Apoptosis Through Autophagic Removal of Death Receptor 5: Evidence from an In Vitro Model Cancers (Basel) 1/15/2019 [PMID: 30650534]
• C McCann, N Crawford, J Majkut, C Holohan, CWD Armstrong, PJ Maxwell, CW Ong, MJ LaBonte, SS McDade, DJ Waugh, DB Longley Cytoplasmic FLIP(S) and nuclear FLIP(L) mediate resistance of castrate-resistant prostate cancer to apoptosis induced by IAP antagonists Cell Death Dis, 2018-10-22;9(11):1081. 2018-10-22 [PMID: 30349042] (Flow Cytometry, Human)
• A Fauster, M Rebsamen, KL Willmann, A César-Razq, E Girardi, JW Bigenzahn, F Schischlik, S Scorzoni, M Bruckner, J Konecka, K Hörmann, LX Heinz, K Boztug, G Superti-Fu Systematic genetic mapping of necroptosis identifies SLC39A7 as modulator of death receptor trafficking Cell Death Differ., 2018-09-20;0(0):. 2018-09-20 [PMID: 30237509] (Flow Cytometry, Human)
• Fairclough L, Stoop A, Negm O, Radford P, Tighe P, Todd I Tumour necrosis factor receptor I blockade shows that TNF-dependent and TNF-independent mechanisms synergise in TNF receptor associated periodic syndrome. Eur J Immunol, 2015-08-17;45(10):2937-44. 2015-08-17 [PMID: 26202849] (Flow Cytometry, Human)
• Tufa D, Chatterjee D, Low H, Schmidt R, Jacobs R TNFR2 and IL-12 coactivation enables slanDCs to support NK-cell function via membrane-bound TNF-alpha. Eur J Immunol, 2014-10-29;44(12):3717-28. 2014-10-29 [PMID: 25229755] (Flow Cytometry, Human)
• Sergey V. Sennikov, Filipp F. Vasilyev, Julia A. Lopatnikova, Nadezhda S. Shkaruba, Alexander N. Silkov Polymorphisms in the Tumor Necrosis Factor Receptor Genes Affect the Expression Levels of Membrane-Bound Type I and Type II Receptors Mediators of Inflammation 2014-03-24 [PMID: 24782596]
• Ruspi G, Schmidt E, McCann F, Feldmann M, Williams R, Stoop A, Dean J TNFR2 increases the sensitivity of ligand-induced activation of the p38 MAPK and NF-kappaB pathways and signals TRAF2 protein degradation in macrophages. Cell Signal, 2013-12-27;26(4):683-90. 2013-12-27 [PMID: 24378531] (Flow Cytometry, Human)
• Isabelle Jéru, Serge Charmion, Emmanuelle Cochet, Bruno Copin, Philippe Duquesnoy, Maria Teresa Mitjavila Garcia et al. Involvement of the Same TNFR1 Residue in Mendelian and Multifactorial Inflammatory Disorders PLoS ONE 2013-07-24 [PMID: 23894535]
• Cosgrove C, Ussher J, Rauch A, Gartner K, Kurioka A, Huhn M, Adelmann K, Kang Y, Fergusson J, Simmonds P, Goulder P, Hansen T, Fox J, Gunthard H, Khanna N, Powrie F, Steel A, Gazzard B, Phillips R, Frater J, Uhlig H, Klenerman P Early and nonreversible decrease of CD161++ /MAIT cells in HIV infection. Blood, 2012-12-18;121(6):951-61. 2012-12-18 [PMID: 23255555] (Flow Cytometry, Human)
• Jun-Jie Chen, H.-C. Jennifer Shen, Leslie A. Rivera Rosado, Yaqin Zhang, Xu Di, Baolin Zhang Mislocalization of death receptors correlates with cellular resistance to their cognate ligands in human breast cancer cells Oncotarget 2012-08-19 [PMID: 22909995]
• Johansson AC, Nandakumar KS, Persson AM, Olsson I, Hansson M Secretory lysosome targeting and induced secretion of human soluble TNF-alpha receptor in murine hematopoietic cells in vivo as a principle for immunoregulation in inflammation and malignancy. Exp. Hematol., 2009-05-30;37(8):969-78. 2009-05-30 [PMID: 19486922] (Flow Cytometry, Mouse)
• Trevor L. Nydam, Ernest E. Moore, Robert C. McIntyre, Franklin L. Wright, Fabia Gamboni-Robertson, Phillip C. Eckels et al. Hypertonic saline attenuates TNF-alpha-induced NF-kappaB activation in pulmonary epithelial cells Shock 2018-01-31 [PMID: 18948845]
• Liu LY, Bates ME, Jarjour NN, Busse WW, Bertics PJ, Kelly EA Generation of Th1 and Th2 chemokines by human eosinophils: evidence for a critical role of TNF-alpha. J. Immunol., 2007-10-01;179(7):4840-8. 2007-10-01 [PMID: 17878383] (Flow Cytometry, Human)
• Rossol M, Meusch U, Pierer M, Kaltenhauser S, Hantzschel H, Hauschildt S, Wagner U Interaction between transmembrane TNF and TNFR1/2 mediates the activation of monocytes by contact with T cells. J. Immunol., 2007-09-15;179(6):4239-48. 2007-09-15 [PMID: 17785864] (Flow Cytometry, Human)
• Montag C, Wagner J, Gruska I, Hagemeier C Human cytomegalovirus blocks tumor necrosis factor alpha- and interleukin-1beta-mediated NF-kappaB signaling. J. Virol., 2006-09-27;80(23):11686-98. 2006-09-27 [PMID: 17005669] (Flow Cytometry, Human)
• Rebelo SL, Bainbridge SE, Amel-Kashipaz MR, Radford PM, Powell RJ, Todd I, Tighe PJ Modeling of tumor necrosis factor receptor superfamily 1A mutants associated with tumor necrosis factor receptor-associated periodic syndrome indicates misfolding consistent with abnormal function. Arthritis Rheum., 2006-08-01;54(8):2674-87. 2006-08-01 [PMID: 16871532] (Flow Cytometry, Human)
• D'Osualdo A Neutrophils from patients with TNFRSF1A mutations display resistance to tumor necrosis factor-induced apoptosis: pathogenetic and clinical implications. Arthritis Rheum., 2006-03-01;54(3):998-1008. 2006-03-01 [PMID: 16508982] (Flow Cytometry, Human)
• Saito K, Hirokawa M, Inaba K, Fukaya H, Kawabata Y, Komatsuda A, Yamashita J, Sawada K Phagocytosis of codeveloping megakaryocytic progenitors by dendritic cells in culture with thrombopoietin and tumor necrosis factor-alpha and its possible role in hemophagocytic syndrome. Blood, 2005-10-18;107(4):1366-74. 2005-10-18 [PMID: 16234354] (Flow Cytometry, Human)
• Fukaya H, Xiao W, Inaba K, Suzuki Y, Hirokawa M, Kawabata Y, Komatsuda A, Endo T, Kishimoto H, Takada G, Sawada K Codevelopment of dendritic cells along with erythroid differentiation from human CD34(+) cells by tumor necrosis factor-alpha. Exp. Hematol., 2004-05-01;32(5):450-60. 2004-05-01 [PMID: 15145213] (Flow Cytometry, Human)
• Xiao W, Koizumi K, Nishio M, Endo T, Osawa M, Fujimoto K, Sato I, Sakai T, Koike T, Sawada K Tumor necrosis factor-alpha inhibits generation of glycophorin A+ cells by CD34+ cells. Exp. Hematol., 2002-11-01;30(11):1238-47. 2002-11-01 [PMID: 12423676] (Flow Cytometry, Human)
• Segura I, 2019, Serrano A, De Buitrago GG, Gonzalez MA, Abad JL, Claveria C, Gomez L, Bernad A, Martinez-A C, Riese HH Inhibition of programmed cell death impairs in vitro vascular-like structure formation and reduces in vivo angiogenesis. 7096, 2002-06-01;16(8):833-41. 2002-06-01 [PMID: 12039865] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: TNFRSF1A
• Uniprot:
  • NP_001056()