TNF-alpha Antibody [Unconjugated]

Recombinant Human TNF‑ alpha (Catalog # 210-TA) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Human TNF‑ alpha (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Human TNF‑ alpha Polyclonal Antibody (Catalog # AB-210-NA). The ND50 is typically 0.05-0.25 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).

• Reactivity: Hu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

TNF-alpha Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human TNF-alpha
  Val77-Leu233
  Accession # P01375
• Specificity: Detects human TNF-alpha in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant canine TNF‑ alpha , recombinant bovine TNF‑ alpha , recombinant rat TNF‑ alpha , recombinant equine TNF‑ alpha , and recombinant cotton rat TNF‑ alpha is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TNF
• Purity Statement: Protein A or G purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.05-0.25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 1 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TNF-alpha Antibody [Unconjugated]

• APC1 protein
• Cachectin
• Cachetin
• DIF
• TNF
• TNF, monocyte-derived
• TNFA
• TNF-A
• TNFalpha
• TNF-alpha
• TNF-alphacachectin
• TNFATNF, macrophage-derived
• TNFG1F
• TNFSF1A
• TNFSF2
• TNFSF2TNF superfamily, member 2
• tumor necrosis factor (TNF superfamily, member 2)
• tumor necrosis factor alpha
• Tumor necrosis factor ligand superfamily member 2
• tumor necrosis factor
• tumor necrosis factor-alpha

Background

Tumor necrosis factor alpha (TNF-alpha, TNF- alpha , TNFA ), also known as Cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism. TNF- is produced by several lymphoid cells as well as by astrocytes, endothelial cells, and smooth muscle cells. Human TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD). Within the ECD, human TNF-alpha shares 97% aa sequence identity with rhesus and 71%-92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-alpha. TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells. TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface. Cell surface TNF-alpha can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I. Shedding of membrane bound TNF-alpha by TACE/ADAM17 releases the bioactive cytokine, a 55 kDa molecular weight soluble trimer of the TNF-alpha extracellular domain. TNF-alpha binds the ubiquitous 55-60 kDa TNF RI and the hematopoietic cell-restricted 80 kDa TNF RII, both of which are also expressed as homotrimers present on virtually all cell types. Both type I and type II receptors bind TNF-alpha with comparable affinity, although only TNF RI contains a cytoplasmic death domain which triggers the activation of apoptosis. Soluble forms of both types of receptors are released and can neutralize the biological activity of TNF-alpha.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TNF-alpha Antibody (AB-210-NA)(11)

Publications using AB-210-NA

• Sytu, MRC;Stoner, A;Hahm, JI; Strain-Modulated and Nanorod-Waveguided Fluorescence in Single Zinc Oxide Nanorod-Based Immunodetection Biosensors 2024-02-03 [PMID: 38392004] (ELISA Detection, N/A)
• Guang Liu, Courtney M. Shea, Juli E. Jones, Gavrielle M. Price, William Warren, Elisabeth Lonie et al. Praliciguat inhibits progression of diabetic nephropathy in ZSF1 rats and suppresses inflammation and apoptosis in human renal proximal tubular cells American Journal of Physiology-Renal Physiology 10/1/2020 [PMID: 32865013]
• Manpreet Singh, Anginelle Alabanza, Lorelis E. Gonzalez, Weiwei Wang, W. Brian Reeves, Jong-in Hahm Ultratrace level determination and quantitative analysis of kidney injury biomarkers in patient samples attained by zinc oxide nanorods Nanoscale 2/28/2016 [PMID: 26846189]
• Zhi-Yang Chang, Ming-Kung Yeh, Chiao-Hsi Chiang, Yi-Hao Chen, Da-Wen Lu Erythropoietin Protects Adult Retinal Ganglion Cells against NMDA-, Trophic Factor Withdrawal-, and TNF-alpha -Induced Damage PLoS ONE 1/30/2013 [PMID: 23383140]
• Zhi-Yang Chang, Ming-Kung Yeh, Chiao-Hsi Chiang, Yi-Hao Chen, Da-Wen Lu Erythropoietin Protects Adult Retinal Ganglion Cells against NMDA-, Trophic Factor Withdrawal-, and TNF-alpha -Induced Damage PLoS ONE 2013-01-30 [PMID: 23383140] (Neutralization, Rat)
• Hubo M et al. Plasmacytoid dendritic cells are inefficient in activation of human regulatory T cells. PLoS One 1/1/2012 [PMID: 22952871]
• W M Liu, D W Fowler, A M Gravett, P Smith, A G Dalgleish Supernatants from lymphocytes stimulated with Bacillus Calmette-Guerin can modify the antigenicity of tumours and stimulate allogeneic T-cell responses British Journal of Cancer 8/23/2011 [PMID: 21829193]
• W M Liu, D W Fowler, A M Gravett, P Smith, A G Dalgleish Supernatants from lymphocytes stimulated with Bacillus Calmette-Guerin can modify the antigenicity of tumours and stimulate allogeneic T-cell responses British Journal of Cancer 2011-08-23 [PMID: 21829193] (Bioassay, Human)
• Liang X, Moseman EA, Farrar MA Toll-like receptor 9 signaling by CpG-B oligodeoxynucleotides induces an apoptotic pathway in human chronic lymphocytic leukemia B cells. Blood, 2010-03-25;115(24):5041-52. 2010-03-25 [PMID: 20339095] (Neutralization, Human)
• Yearley JH, Mansfield KG, Carville AA, Sokos GG, Xia D, Pearson CB, Shannon RP Antigenic stimulation in the simian model of HIV infection yields dilated cardiomyopathy through effects of TNFalpha. AIDS, 2008-03-12;22(5):585-94. 2008-03-12 [PMID: 18316999] (Western Blot, Human)
• Kinasewitz GT, 2016, Chang AC, Peer GT, Hinshaw LB, Taylor FB Peritonitis in the baboon: a primate model which stimulates human sepsis. 2000-02-01;13(2):100-9. 2000-02-01 [PMID: 10670839] (ELISA Development, Primate - Papio cynocephalus (Yellow Baboon))

FAQs for TNF-alpha Antibody (AB-210-NA). (Showing 1 - 4 of 4 FAQs).

1. I need to know about TNF-alpha antibody kits for mice.
   • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
   • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
   • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
   • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

Bioinformatics

• Gene Symbol: TNF
• Uniprot:
  • P01375()