Recombinant Human TNF‑ alpha (Catalog # 210-TA) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Human TNF‑ alpha ...read more
E. coli-derived recombinant human TNF-alpha Val77-Leu233 Accession # P01375
Detects human TNF-alpha in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant canine TNF‑ alpha, recombinant bovine TNF‑ alpha, recombinant rat TNF‑ alpha, recombinant equine TNF‑ alpha, and recombinant cotton rat TNF‑ alpha is observed.
Protein A or G purified
<0.10 EU per 1 μg of the antibody by the LAL method.
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitute at 1 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for TNF-alpha Antibody [Unconjugated]
TNFSF2TNF superfamily, member 2
tumor necrosis factor (TNF superfamily, member 2)
tumor necrosis factor alpha
Tumor necrosis factor ligand superfamily member 2
tumor necrosis factor
tumor necrosis factor-alpha
Tumor necrosis factor alpha (TNF-alpha, TNF- alpha, TNFA ), also
known as Cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily.
It is a pleiotropic molecule that plays a central role in inflammation, immune
system development, apoptosis, and lipid metabolism. TNF- is produced by
several lymphoid cells as well as by astrocytes, endothelial cells, and smooth
muscle cells. Human TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic
domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD).
Within the ECD, human TNF-alpha shares 97% aa sequence identity with rhesus and
71%-92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and
rat TNF-alpha. TNF-alpha is produced by a wide variety of immune, epithelial,
endothelial, and tumor cells. TNF-alpha is assembled intracellularly to form a
noncovalently linked homotrimer which is expressed on the cell surface. Cell
surface TNF-alpha can induce the lysis of neighboring tumor cells and virus
infected cells, and it can generate its own downstream cell signaling following
ligation by soluble TNFR I. Shedding of membrane bound TNF-alpha by TACE/ADAM17
releases the bioactive cytokine, a 55 kDa molecular weight soluble trimer of
the TNF-alpha extracellular domain. TNF-alpha binds the ubiquitous 55-60 kDa
TNF RI and the hematopoietic cell-restricted 80 kDa TNF RII, both of which are
also expressed as homotrimers present on virtually all cell types. Both type I
and type II receptors bind TNF-alpha with comparable affinity, although only
TNF RI contains a cytoplasmic death domain which triggers the activation of
apoptosis. Soluble forms of both types of receptors are released and can
neutralize the biological activity of TNF-alpha.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for TNF-alpha Antibody (AB-210-NA). (Showing 1 - 4 of 4 FAQs).
I need to know about TNF-alpha antibody kits for mice.
We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.
CD86 - I work in tandem with CD80 CD86 belongs to the immunoglobulin superfamily of proteins that drive innate and adaptive immune responses. It is an 80kD co-stimulatory molecule for the priming and activation of naive and memory T-cells, respectively. CD86 is expressed on activated... Read full blog post.
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PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.