TNF-alpha Antibody (TNFA/1172)

Immunocytochemistry/Immunofluorescence: TNF-alpha Antibody (TNFA/1172) [NBP2-45331] - Immunofluorescence Analysis of PFA-fixed HePG2 cells labeling TNF-alpha with TNF-alpha Antibody (TNFA/1172) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot.

Immunohistochemistry-Paraffin: TNF-alpha Antibody (TNFA/1172) [NBP2-45331] - Formalin-fixed, paraffin-embedded human Pancreas stained with TNF-alpha Antibody (TNFA/1172).

Flow Cytometry: TNF-alpha Antibody (TNFA/1172) [NBP2-45331] - Flow Cytometric Analysis of PFA-fixed HePG2 cells using TNF-alpha Antibody (TNFA/1172).followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).

Immunohistochemistry-Paraffin: TNF-alpha Antibody (TNFA/1172) [NBP2-45331] - Human Erdheim Chester disease (also known as polyostotic lerosing histiocytosis) stained with TNF alpha Monoclonal Antibody (TNFA/1172).

Immunohistochemistry-Paraffin: TNF-alpha Antibody (TNFA/1172) [NBP2-45331] - Human Histiocytoma stained with TNF alpha Monoclonal Antibody (TNFA/1172)

Immunohistochemistry-Paraffin: TNF-alpha Antibody (TNFA/1172) [NBP2-45331] - Rat Pancreas stained with TNF alpha Monoclonal Antibody (TNFA/1172)

Immunohistochemistry-Paraffin: TNF-alpha Antibody (TNFA/1172) [NBP2-45331] - Rat Stomach stained with TNF alpha Monoclonal Antibody (TNFA/1172)

• Reactivity: Hu, Rt
• Applications: Flow, ICC/IF, IHC, IHC-P
• Clone: TNFA/1172
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

TNF-alpha Antibody (TNFA/1172) Summary

• Immunogen: Recombinant full-length human TNF-alpha protein
• Localization: Cytoplasmic and extracellular (secreted)
• Specificity: This MAb recognizes human 17-26kDa protein, which is identified as cytokine TNF-alpha (Tumor Necrosis Factor-alpha). TNF-alpha can be expressed as a 17kDa free molecule, or as a 26kDa membrane protein. TNF-alpha is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.
• Isotype: IgM Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNF
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 2 - 4ug/ml
• Application Notes: Hu-chromosome location: 6p21.33
  Immunohistology Formalin-paraffin 2 - 4 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
• Theoretical MW: 17 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for TNF-alpha Antibody (TNFA/1172)

• APC1 protein
• Cachectin
• Cachetin
• DIF
• TNF
• TNF, monocyte-derived
• TNFA
• TNF-A
• TNFalpha
• TNF-alpha
• TNF-alphacachectin
• TNFATNF, macrophage-derived
• TNFG1F
• TNFSF1A
• TNFSF2
• TNFSF2TNF superfamily, member 2
• tumor necrosis factor (TNF superfamily, member 2)
• tumor necrosis factor alpha
• Tumor necrosis factor ligand superfamily member 2
• tumor necrosis factor
• tumor necrosis factor-alpha

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for TNF-alpha Antibody (NBP2-45331). (Showing 1 - 4 of 4 FAQ).

1. I need to know about TNF-alpha antibody kits for mice.
   • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
   • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
   • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
   • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

Bioinformatics

• Gene Symbol: TNF
• Uniprot:
  • P01375()