TNF-alpha Antibody (TN3-19.12) [Unconjugated]

Western blot shows 25 ng of Recombinant Mouse TNF-a aa 80-235 (Catalog # 410-MT), Recombinant Human TNF-a (Catalog # 210-TA), Recombinant Rat TNF-a (Catalog # 510-RT) and Recombinant Mouse Lymphotoxin- a/TNF- beta . PVDF Membrane was probed with 1 µg/mL of Hamster Anti-Mouse TNF-a Monoclonal Antibody (Catalog # MAB410) followed by HRP-conjugated Anti-Hamster IgG Secondary Antibody. A specific band was detected for TNF-a at approximately 15 kDa (as indicated). This experiment was conducted under reducing and non reducing conditions using Immunoblot Buffer Group 3.

Inflammatory response induced by HI injury and maternal SE. Representative images of immunofluorescence staining of inflammatory cytokines IL-1 beta , IL-6, and TNF alpha in the cerebral cortex (A) and the hippocampus (E). The immunofluorescence intensity of IL-1 beta (B,F), IL-6 (C,G), and TNF alpha (D,H) in the cerebral cortex and hippocampus. Results are presented as mean ± SEM. *P < 0.05, **P < 0.01, n = 4, analyzed by two-way ANOVA followed by post hoc Turkey tests. SH, from sham exposed dams with sham surgery; HI, hypoxic-ischemic injury; SE, from smoke exposed dams with sham surgery; HI + SE, from smoke exposed dams with hypoxic-ischemic injury. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35250486), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: WB
• Clone: TN3-19.12
• Clonality: Monoclonal
• Host: Hamster
• Conjugate: Unconjugated

TNF-alpha Antibody (TN3-19.12) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse TNF-alpha
• Specificity: Detects recombinant mouse (rm)TNF-alpha in direct ELISAs and Western blots. Shows 50% cross-reactivity with rrTNF-alpha and no cross-reactivity with 17 other tested proteins.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Hamster
• Gene: TNF
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TNF-alpha Antibody (TN3-19.12) [Unconjugated]

• APC1 protein
• Cachectin
• Cachetin
• DIF
• TNF
• TNF, monocyte-derived
• TNFA
• TNF-A
• TNFalpha
• TNF-alpha
• TNF-alphacachectin
• TNFATNF, macrophage-derived
• TNFG1F
• TNFSF1A
• TNFSF2
• TNFSF2TNF superfamily, member 2
• tumor necrosis factor (TNF superfamily, member 2)
• tumor necrosis factor alpha
• Tumor necrosis factor ligand superfamily member 2
• tumor necrosis factor
• tumor necrosis factor-alpha

Background

Tumor necrosis factor alpha (TNF-alpha, TNF- alpha , TNFA ), also known as Cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism. TNF-alpha is produced by several lymphoid cells as well as by astrocytes, endothelial cells, and smooth muscle cells. Mouse TNF-alpha consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD). Within the ECD, mouse TNF-alpha shares 94% aa sequence identity with rat and 70%-77% with bovine, canine, cotton rat, equine, feline, human, porcine, and rhesus TNF-alpha. TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells. TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface. Cell surface TNF-alpha can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I. Shedding of membrane bound TNF-alpha by TACE/ADAM17 releases the bioactive cytokine, a 55 kDa molecular weight soluble trimer of the TNF-alpha extracellular domain. TNF-alpha binds the ubiquitous 55-60 kDa TNF RI and the hematopoietic cell-restricted 80 kDa TNF RII, both of which are also expressed as homotrimers present on virtually all cell types. Both type I and type II receptors bind TNF-alpha with comparable affinity, although only TNF RI contains a cytoplasmic death domain which triggers the activation of apoptosis. Soluble forms of both types of receptors are released and can neutralize the biological activity of TNF-alpha.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TNF-alpha Antibody (MAB410)(7)

Publications using MAB410

• Taida Huang, Xiaomin Huang, Hui Li, Junhua Qi, Nan Wang, Yi Xu et al. Maternal Cigarette Smoke Exposure Exaggerates the Behavioral Defects and Neuronal Loss Caused by Hypoxic-Ischemic Brain Injury in Female Offspring Frontiers in Cellular Neuroscience 2/18/2022 [PMID: 35250486]
• Naixin Song, Pengfei Xie, Wen Shen, Hanju Oh, Yejia Zhang, Flavia Vitale et al. A microwell-based impedance sensor on an insertable microneedle for real-time in vivo cytokine detection Microsystems & Nanoengineering 11/26/2021 [PMID: 34900330]
• Wunnie Brima, Daniel J. Eden, Syed Faizan Mehdi, Michelle Bravo, Mohammad M. Wiese, Joanna Stein et al. The brighter (and evolutionarily older) face of the metabolic syndrome: evidence from Trypanosoma cruzi infection in CD-1 mice Diabetes/Metabolism Research and Reviews 5/1/2015 [PMID: 25613819]
• Marut W, Kavian N, Servettaz A, Hua-Huy T, Nicco C, Chereau C, Weill B, Dinh-Xuan A, Batteux F Amelioration of systemic fibrosis in mice by angiotensin II receptor blockade. Arthritis Rheum, 2013-05-01;65(5):1367-77. 2013-05-01 [PMID: 23335130] (Western Blot, Mouse)
• Yimin, Kohanawa M, Ozaki M, Haga S, Fujikawa K, Zhao S, Kuge Y, Tamaki N Mutual modulation between interleukin-10 and interleukin-6 induced by Rhodococcus aurantiacus infection in mice. Microbes Infect., 2008-09-13;10(14):1450-8. 2008-09-13 [PMID: 18834949] (Neutralization, Mouse)
• Ito H, Ando K, Ishikawa T Role of Valpha14+ NKT cells in the development of Hepatitis B virus-specific CTL: activation of Valpha14+ NKT cells promotes the breakage of CTL tolerance. Int. Immunol., 2008-05-16;20(7):869-79. 2008-05-16 [PMID: 18487227] (Neutralization, Mouse)
• Figiel I, Dzwonek K TNFalpha and TNF receptor 1 expression in the mixed neuronal-glial cultures of hippocampal dentate gyrus exposed to glutamate or trimethyltin. Brain Res., 2006-12-11;1131(1):17-28. 2006-12-11 [PMID: 17161388] (Western Blot, Rat)

FAQs for TNF-alpha Antibody (MAB410). (Showing 1 - 4 of 4 FAQs).

1. I need to know about TNF-alpha antibody kits for mice.
   • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
   • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
   • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
   • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

Bioinformatics

• Gene Symbol: TNF