TNF-alpha Antibody (J2D10)

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SDS-Page: TNF-alpha Antibody (J2D10) [NBP2-45333]

Product Details

Summary
Reactivity Hu, Rt, Mu(-)Species Glossary
Applications Flow, PAGE, ICC/IF
Clone
J2D10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

TNF-alpha Antibody (J2D10) Summary

Immunogen
Recombinant human TNF-alpha
Localization
Cytoplasmic and extracellular (secreted)
Specificity
This antibody neutralises HurTNFamediated cytotoxicity of L929 cells and inhibits tumour growth in mice. It protects mice against toxicity of HurTNFa. Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TNF
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • SDS-Page
  • Immunofluorescence 1 - 2 ug/ml
Application Notes
Hu-chromosome location: 6p21.33
Inhibits Tumor Growth Order Ab without BSA/Azide; Neutralizes rTNF-alpha Mediated Cytotoxicity Order Ab without BSA/Azide; Protects Mice Against Toxicity of rhTNF-alpha Order Ab without BSA/Azide
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-45333 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10mM PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for TNF-alpha Antibody (J2D10)

  • APC1 protein
  • Cachectin
  • Cachetin
  • DIF
  • TNF
  • TNF, monocyte-derived
  • TNFA
  • TNF-A
  • TNFalpha
  • TNF-alpha
  • TNF-alphacachectin
  • TNFATNF, macrophage-derived
  • TNFG1F
  • TNFSF1A
  • TNFSF2
  • TNFSF2TNF superfamily, member 2
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • Tumor necrosis factor ligand superfamily member 2
  • tumor necrosis factor
  • tumor necrosis factor-alpha

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-45333
Species: Hu, Rt, Mu(-)
Applications: Flow, PAGE, ICC/IF

Publications for TNF-alpha Antibody (NBP2-45333)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
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Rat
(1)
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Product General Protocols

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FAQs for TNF-alpha Antibody (NBP2-45333). (Showing 1 - 4 of 4 FAQ).

  1. I need to know about TNF-alpha antibody kits for mice.
    • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
  2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
    • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
  3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
    • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
  4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
    • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-47677AF350
Alexa Fluor 405 NBP2-47677AF405
Alexa Fluor 488 NBP2-47677AF488
Alexa Fluor 532 NBP2-47677AF532
Alexa Fluor 594 NBP2-47677AF594
Alexa Fluor 647 NBP2-47677AF647
Alexa Fluor 700 NBP2-47677AF700
Alexa Fluor 750 NBP2-47677AF750
Allophycocyanin NBP2-47677APC
Allophycocyanin/Cy7 NBP2-47677APCCY7
DyLight 350 NBP2-47677UV
DyLight 405 NBP2-47677V
DyLight 488 NBP2-47677G
DyLight 550 NBP2-47677R
DyLight 594 NBP2-47677DL594
DyLight 650 NBP2-47677C
DyLight 680 NBP2-47677FR
DyLight 755 NBP2-47677IR
FITC NBP2-47677F
Janelia Fluor 549 NBP2-47677JF549
Janelia Fluor 646 NBP2-47677JF646
PE NBP2-47677PE
PE/Atto594 NBP2-47677PEATT594
PE/Cy5.5 NBP2-47677PECY55
PE/Cy7 NBP2-47677PECY7
PerCP NBP2-47677PCP

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Blogs on TNF-alpha.

You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers
By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t...  Read full blog post.

Friends become Foes: Molecular Chaperons, Hsp70 and Hsp90, Cause Muscle Wasting in Cancers
By Jamshed Arslan Pharm.D. Muscle atrophy is a common feature of many tumors. Cancer-induced muscle wasting, or cancer cachexia, results from pro-in?ammatory cytokines (TNFa and IL-6) and/or agonists of type IIB ac...  Read full blog post.

cIAP2 - balancing cell death and cell survival
The inhibitor of apoptosis proteins (IAPs) are important regulators of cell death and inflammation. The cellular inhibitor of apoptosis protein 2 (cIAP2) contains three Baculovirus IAP repeat (BIR) domains, a Ubiquitin associated (UBA) domain, and...  Read full blog post.

CD86 - I work in tandem with CD80
CD86 belongs to the immunoglobulin superfamily of proteins that drive innate and adaptive immune responses. It is an 80kD co-stimulatory molecule for the priming and activation of naive and memory T-cells, respectively. CD86 is expressed on activated...  Read full blog post.

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Bioinformatics

Gene Symbol TNF
Uniprot