TNF-alpha Antibody (6N2E12) [DyLight 550]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow
Clone
6N2E12
Clonality
Monoclonal
Host
Mouse

TNF-alpha Antibody (6N2E12) [DyLight 550] Summary

Immunogen
Full-length recombinant human TNF alpha was used as the immunogen for this antibody.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TNF
Purity
Protein G purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
DyLight 550
Format
DyLight 550 (A=562, E=576)
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry 1ul/1 million cells

Reactivity Notes

Human

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for TNF-alpha Antibody (6N2E12) [DyLight 550]

  • APC1 protein
  • Cachectin
  • Cachetin
  • DIF
  • TNF
  • TNF, monocyte-derived
  • tnfa
  • tnf-a
  • TNFalpha
  • TNF-alpha
  • TNF-alphacachectin
  • TNFATNF, macrophage-derived
  • TNFSF1A
  • TNFSF2
  • TNFSF2TNF superfamily, member 2
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • Tumor necrosis factor ligand superfamily member 2
  • tumor necrosis factor
  • tumor necrosis factor-alpha

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TNF-alpha Antibody (NBP2-27351R). (Showing 1 - 4 of 4 FAQ).

  1. I need to know about TNF-alpha antibody kits for mice.
    • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
  2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
    • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
  3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
    • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
  4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
    • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 647 Labeled NBP2-27270
Allophycocyanin Labeled NBP2-27351APC
Biotin Labeled NBP2-27351B
DyLight 350 Labeled NBP2-27351UV
DyLight 405 Labeled NBP2-27351V
DyLight 405LS Labeled NBP2-27351V3
DyLight 488 Labeled NBP2-27351G
DyLight 650 Labeled NBP2-27351C
DyLight 680 Labeled NBP2-27351FR
DyLight 755 Labeled NBP2-27351IR
HRP Labeled NBP2-27351H
PE Labeled NBP2-27351PE
PerCP Labeled NBP2-27351PCP

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Blogs on TNF-alpha.

cIAP2 - balancing cell death and cell survival
The inhibitor of apoptosis proteins (IAPs) are important regulators of cell death and inflammation. The cellular inhibitor of apoptosis protein 2 (cIAP2) contains three Baculovirus IAP repeat (BIR) domains, a Ubiquitin associated (UBA) domain, and...  Read full blog post.

CD86 - I work in tandem with CD80
CD86 belongs to the immunoglobulin superfamily of proteins that drive innate and adaptive immune responses. It is an 80kD co-stimulatory molecule for the priming and activation of naive and memory T-cells, respectively. CD86 is expressed on activated...  Read full blog post.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol TNF
Entrez
Uniprot

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