TNF-alpha Antibody (28401) [Unconjugated]

Images

 
Western blot shows 25 ng of Recombinant Human TNF‑ alpha (Catalog # 210-TA), Recombinant Mouse TNF‑ alpha aa 80-235 (Catalog # 410-MT), Recombinant Rat TNF‑ alpha (Catalog # 510-RT), and Recombinant ...read more
Recombinant Human TNF‑ alpha (Catalog #210‑TA) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Human TNF‑ alpha ...read more
TNF‑ alpha was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with LPS and monensin using Mouse Anti-Human TNF‑ alpha Monoclonal Antibody (Catalog # MAB610) at 10 µg/mL ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N, ELISA(Cap), ICC/IF
Clone
28401
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price

TNF-alpha Antibody (28401) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human TNF-alpha
Gly57-Leu233 (predicted)
Accession # P01375
Specificity
Detects human TNF-alpha in ELISAs and Western blots. In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant human TNF‑ beta, recombinant mouse TNF‑ alpha, recombinant rat TNF‑ alpha, and recombinant porcine TNF‑ alpha is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TNF
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Immunocytochemistry 8-25 ug/mL
  • Neutralization 0.01-0.04 ug/mL
Application Notes
ELISA Detection: Human TNF-alpha Biotinylated Antibody (Catalog number BAF210)
Standard: Recombinant Human TNF-alpha (Catalog number 210-TA)
Reviewed Applications
Read 1 Review rated 5
using
MAB610 in the following applications:

Publications
Read Publications using
MAB610 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TNF-alpha Antibody (28401) [Unconjugated]

  • APC1 protein
  • Cachectin
  • Cachetin
  • DIF
  • TNF
  • TNF, monocyte-derived
  • TNFA
  • TNF-A
  • TNFalpha
  • TNF-alpha
  • TNF-alphacachectin
  • TNFATNF, macrophage-derived
  • TNFG1F
  • TNFSF1A
  • TNFSF2
  • TNFSF2TNF superfamily, member 2
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • Tumor necrosis factor ligand superfamily member 2
  • tumor necrosis factor
  • tumor necrosis factor-alpha

Background

Tumor necrosis factor alpha (TNF-alpha, TNF- alpha, TNFA ), also known as Cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism. TNF- is produced by several lymphoid cells as well as by astrocytes, endothelial cells, and smooth muscle cells. Human TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD). Within the ECD, human TNF-alpha shares 97% aa sequence identity with rhesus and 71%-92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-alpha. TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells. TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface. Cell surface TNF-alpha can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I. Shedding of membrane bound TNF-alpha by TACE/ADAM17 releases the bioactive cytokine, a 55 kDa molecular weight soluble trimer of the TNF-alpha extracellular domain. TNF-alpha binds the ubiquitous 55-60 kDa TNF RI and the hematopoietic cell-restricted 80 kDa TNF RII, both of which are also expressed as homotrimers present on virtually all cell types. Both type I and type II receptors bind TNF-alpha with comparable affinity, although only TNF RI contains a cytoplasmic death domain which triggers the activation of apoptosis. Soluble forms of both types of receptors are released and can neutralize the biological activity of TNF-alpha.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TNF-alpha Antibody (MAB610)(39)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 16 applications: Affinity Chromatography, Antibody Array Development, Array Development, Bioassay, ELISA Capture, ELISA Development, ELISA Development (Capture), ELISA Microarray Development, Electrochemiluminescent Assay, ICC, IHC, IHC-Fr, IHC-P, Luminex Development, Neutralization, Western Blot.


Filter By Application
Affinity Chromatography
(1)
Antibody Array Development
(1)
Array Development
(1)
Bioassay
(1)
ELISA Capture
(1)
ELISA Development
(11)
ELISA Development (Capture)
(1)
ELISA Microarray Development
(1)
Electrochemiluminescent Assay
(1)
ICC
(2)
IHC
(2)
IHC-Fr
(3)
IHC-P
(1)
Luminex Development
(3)
Neutralization
(7)
Western Blot
(2)
All Applications
Filter By Species
Human
(37)
Mouse
(2)
All Species
Showing Publications 1 - 10 of 39. Show All 39 Publications.
Publications using MAB610 Applications Species
A Stumpf, T Brandstett, J Hübner, J Rühe Hydrogel based protein biochip for parallel detection of biomarkers for diagnosis of a Systemic Inflammatory Response Syndrome (SIRS) in human serum PLoS ONE, 2019;14(12):e0225525. 2019 [PMID: 31790441] (ELISA Capture, Human) ELISA Capture Human
E Hoste, C Maueröder, L van Hove, L Catrysse, HK Vikkula, M Sze, B Maes, D Karjosukar, L Martens, A Gonçalves, E Parthoens, R Roelandt, W Declercq, I Fuentes, F Palisson, S Gonzalez, JC Salas-Alan, L Boon, P Huebener, KW Mulder, K Ravichandr, Y Saeys, RF Schwabe, G van Loo Epithelial HMGB1 Delays Skin Wound Healing and Drives Tumor Initiation by Priming Neutrophils for NET Formation Cell Rep, 2019;29(9):2689-2701.e4. 2019 [PMID: 31775038] (IHC, Mouse) IHC Mouse
W Sun, N Meednu, A Rosenberg, J Rangel-Mor, V Wang, J Glanzman, T Owen, X Zhou, H Zhang, BF Boyce, JH Anolik, L Xing B cells inhibit bone formation in rheumatoid arthritis by suppressing osteoblast differentiation Nat Commun, 2018;9(1):5127. 2018 [PMID: 30510188] (Neutralization, Mouse) Neutralization Mouse
Y Wang, J Liu, X Chen, H Sun, S Peng, Y Kuang, J Pi, T Zhuang, L Zhang, Z Yu, B Tomlinson, P Chan, Y Chen, Y Zhang, Y Li Dysfunctional endothelial-derived microparticles promote inflammatory macrophage formation via NF-?B and IL-1? signal pathways J. Cell. Mol. Med., 2018;0(0):. 2018 [PMID: 30334371] (Western Blot, Human) Western Blot Human
S Urra, MC Fischer, JR Martínez, L Véliz, P Orellana, A Solar, K Bohmwald, A Kalergis, C Riedel, AH Corvalán, JC Roa, R Fuentealba, CJ Cáceres, M López-Last, A León, N Droppelman, HE González Differential expression profile of CXCR3 splicing variants is associated with thyroid neoplasia. Potential role in papillary thyroid carcinoma oncogenesis? Oncotarget, 2018;9(2):2445-2467. 2018 [PMID: 29416784] (Western Blot, Human) Western Blot Human
K Ouhara, S Munenaga, M Kajiya, K Takeda, S Matsuda, Y Sato, Y Hamamoto, T Iwata, S Yamasaki, K Akutagawa, N Mizuno, T Fujita, E Sugiyama, H Kurihara The induced RNA-binding protein, HuR targets 3&#039;-UTR region of IL-6 mRNA and enhances its stabilization in periodontitis Clin. Exp. Immunol., 2018;0(0):. 2018 [PMID: 29393507] (Neutralization, Human) Neutralization Human
T Regan, AC Gill, SM Clohisey, MW Barnett, CM Pariante, NA Harrison,, DA Hume, ET Bullmore, TC Freeman Effects of anti-inflammatory drugs on the expression of tryptophan-metabolism genes by human macrophages J. Leukoc. Biol., 2018;103(4):681-692. 2018 [PMID: 29377288] (Bioassay, Human) Bioassay Human
CM Henry, SJ Martin Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory FADDosome Complex upon TRAIL Stimulation Mol. Cell, 2017;65(4):715-729.e5. 2017 [PMID: 28212752] (ICC, Human) ICC Human
MC Tanzer, N Khan, JA Rickard, N Etemadi, N Lalaoui, SK Spall, JM Hildebrand, D Segal, M Miasari, D Chau, WL Wong, M McKinlay, SK Chunduru, CA Benetatos, SM Condon, JE Vince, MJ Herold, J Silke Combination of IAP antagonist and IFN? activates novel caspase-10- and RIPK1-dependent cell death pathways Cell Death Differ, 2017;0(0):. 2017 [PMID: 28106882] (Neutralization, Human) Neutralization Human
H Jiang, X Zhang, H Lin Lysine fatty acylation promotes lysosomal targeting of TNF-? Sci Rep, 2016;6(0):24371. 2016 [PMID: 27079798] (IHC-Fr, Human) IHC-Fr Human
Show All 39 Publications.

Review for TNF-alpha Antibody (MAB610) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB610:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot TNF-alpha MAB610
Enlarge
5
reviewed by:
Anonymous
WB Human 04/20/2017
View

Summary

ApplicationWestern Blot
Sample TestedHuman breast cancer cell lysate (MCF-7)
SpeciesHuman
LotAVR1716011

Comments

CommentsMCF-7 cell extract probed with 1 ug/mL of Mouse anti-Human TNF-alpha Antibody (#MAB610) showing a specific band at 18 kDa. Membrane blocked with 5%BSA in PBS. Buffer: 5% BSA in PBS. Secondary Ab: anti-Mouse IgG 1:5,000.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TNF-alpha Antibody (MAB610). (Showing 1 - 4 of 4 FAQs).

  1. I need to know about TNF-alpha antibody kits for mice.
    • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
  2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
    • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
  3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
    • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
  4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
    • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

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Blogs on TNF-alpha.

Liver ASK1 activates autophagy to protect against hepatic fat accumulation, non-alcoholic steatohepatitis and fibrosis
By Jamshed Arslan, Pharm. D., PhD. The most common chronic liver disorder worldwide is non-alcoholic fatty liver disease (NAFLD). This obesity-linked disorder can manifest as hepatic fat accumulation (steatosis) wit...  Read full blog post.

Metabolic Syndrome: Symptoms and associated disease states
By Jamshed Arslan, Pharm. D., PhD. What is metabolic syndrome?It was 1988 when, after decades of research, Dr. GM Reaven    of the Stanford University, CA, explained the relationship between four diseases:...  Read full blog post.

Increased wild type FUS levels in ALS patients lead to a toxic microenvironment and motor neuron neurodegeneration
By Michalina Hanzel, PhDFUS mutations in Amyotrophic Lateral SclerosisFused in sarcoma (FUS) is a ribonucleoprotein that continuously shuttles between the nucleus and the cytoplasm to regulate pre-mRNA splicing, mRN...  Read full blog post.

You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers
By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t...  Read full blog post.

Friends become Foes: Molecular Chaperons, Hsp70 and Hsp90, Cause Muscle Wasting in Cancers
By Jamshed Arslan Pharm.D. Muscle atrophy is a common feature of many tumors. Cancer-induced muscle wasting, or cancer cachexia, results from pro-in?ammatory cytokines (TNFa and IL-6) and/or agonists of type IIB ac...  Read full blog post.

cIAP2 - balancing cell death and cell survival
The inhibitor of apoptosis proteins (IAPs) are important regulators of cell death and inflammation. The cellular inhibitor of apoptosis protein 2 (cIAP2) contains three Baculovirus IAP repeat (BIR) domains, a Ubiquitin associated (UBA) domain, and...  Read full blog post.

CD86 - I work in tandem with CD80
CD86 belongs to the immunoglobulin superfamily of proteins that drive innate and adaptive immune responses. It is an 80kD co-stimulatory molecule for the priming and activation of naive and memory T-cells, respectively. CD86 is expressed on activated...  Read full blog post.

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
04/20/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol TNF
Uniprot