TNF-alpha Antibody (28401) [Unconjugated] Summary
E. coli-derived recombinant human TNF-alpha
Accession # P01375
Detects human TNF-alpha in ELISAs and Western blots. In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant human TNF‑ beta, recombinant mouse TNF‑ alpha, recombinant rat TNF‑ alpha, and recombinant porcine TNF‑ alpha is observed.
<0.10 EU per 1 μg of the antibody by the LAL method.
Test in a species/application not listed above to receive a full credit towards a future purchase.
- Western Blot 1 ug/mL
- ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
- ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
- Immunocytochemistry 8-25 ug/mL
- Neutralization 0.01-0.04 ug/mL
ELISA Detection: Human TNF-alpha Biotinylated Antibody (Catalog number BAF210)
Standard: Recombinant Human TNF-alpha (Catalog number 210-TA)
Packaging, Storage & Formulations
|Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.5 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for TNF-alpha Antibody (28401) [Unconjugated]
- APC1 protein
- TNF, monocyte-derived
- TNFATNF, macrophage-derived
- TNFSF2TNF superfamily, member 2
- tumor necrosis factor (TNF superfamily, member 2)
- tumor necrosis factor alpha
- Tumor necrosis factor ligand superfamily member 2
- tumor necrosis factor
- tumor necrosis factor-alpha
Tumor necrosis factor alpha (TNF-alpha, TNF- alpha, TNFA ), also
known as Cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily.
It is a pleiotropic molecule that plays a central role in inflammation, immune
system development, apoptosis, and lipid metabolism. TNF- is produced by
several lymphoid cells as well as by astrocytes, endothelial cells, and smooth
muscle cells. Human TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic
domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD).
Within the ECD, human TNF-alpha shares 97% aa sequence identity with rhesus and
71%-92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and
rat TNF-alpha. TNF-alpha is produced by a wide variety of immune, epithelial,
endothelial, and tumor cells. TNF-alpha is assembled intracellularly to form a
noncovalently linked homotrimer which is expressed on the cell surface. Cell
surface TNF-alpha can induce the lysis of neighboring tumor cells and virus
infected cells, and it can generate its own downstream cell signaling following
ligation by soluble TNFR I. Shedding of membrane bound TNF-alpha by TACE/ADAM17
releases the bioactive cytokine, a 55 kDa molecular weight soluble trimer of
the TNF-alpha extracellular domain. TNF-alpha binds the ubiquitous 55-60 kDa
TNF RI and the hematopoietic cell-restricted 80 kDa TNF RII, both of which are
also expressed as homotrimers present on virtually all cell types. Both type I
and type II receptors bind TNF-alpha with comparable affinity, although only
TNF RI contains a cytoplasmic death domain which triggers the activation of
apoptosis. Soluble forms of both types of receptors are released and can
neutralize the biological activity of TNF-alpha.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for TNF-alpha Antibody (MAB610). (Showing 1 - 4 of 4 FAQs).
I need to know about TNF-alpha antibody kits for mice.
- We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
- I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
- The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
- I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.
Other Available Formats
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Bioinformatics Tool for TNF-alpha Antibody (MAB610)
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