TMPRSS2 Antibody

Western Blot: TMPRSS2 Antibody [NBP1-20984] - Western blot analysis of Human pancreas and kidney lysates (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: TMPRSS2 Antibody [NBP1-20984] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.

Immunohistochemistry-Paraffin: TMPRSS2 Antibody [NBP1-20984] - Staining of paraffin embedded Human Kidney. Antibody at 8 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Simple Western: TMPRSS2 Antibody [NBP1-20984] - Simple Western lane view (A) and electropherogram view (B) show recombinant human TMPRSS2 Protein (Catalog # H00007113-Q01) loaded at a serial dilution. A specific band was detected for TMPRSS2 at approximately 45 kDa (as indicated) using 1:50 of Goat Polyclonal (Catalog # NBP1-20984). (C) The calculated dynamic range had an R-squared value of 0.9997 with assay sensitivity down to 6.2 ng/mL. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TMPRSS2 Antibody Summary

• Immunogen: This TMPRSS2 Antibody was developed against a peptide with sequence C-TDWIYRQMRADG corresponding to internal region according to NP_005647.2.
• Epitope: C terminus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TMPRSS2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 6 - 8 ug/mL
  • Immunohistochemistry-Paraffin 6 - 8 ug/mL
  • Peptide ELISA Detection limit 1:8000
  • Simple Western
  • Western Blot 0.1 - 0.5 ug/mL
• Application Notes: Western blot: Approx 55-60 kDa band observed in Human pancreas and kidney lysates (calculated MW of 53.9 kDa according to NP_005647.2). Use in ICC/IF reported in scientific publication (PMID: 32511360).
• Theoretical MW: 53.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected from sequence similarity: Mouse (82%)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TMPRSS2 Antibody

• EC 3.4.21
• Epitheliasin
• FLJ41954
• NP_001128571.1
• NP_001369649.1
• NP_005647.3
• PP9284
• PRSS10
• Serine protease 10
• TMPRSS2
• transmembrane protease serine 2
• transmembrane protease, serine 2

Background

TMPRSS2, also called Epitheliasin in mice, is a 492 amino acid type II transmembrane serine protease located on human chromosome 21q22.3 that encodes at least 2 isoforms. This glycosylated serine protease (theoretical molecular weight 70kDa) is regulated by androgens and expressed on the plasma membrane of human bronchial epithelial cells, nasal goblet cells, small intestine epithelia, gastrointestinal tract, the stomach, kidneys and pancreas, with the greatest abundance found in the prostate gland. While the physiological function of TMPRSS2 remains unknown, the serine protease domain undergoes autocleavage and the 32 kDa domain is secreted enabling its interaction with the extracellular matrix. Fusions between TMPRSS2 and the ETS transcription factor genes ERG, ETV1, and ETV4 have been reported in prostate cancer with the TMPRSS2 ERG gene fusion resulting in ERG overexpression in 40-80% of these cases and often producing a more aggressive phenotype. Androgen signaling is disrupted in prostate cancers with the TMPRSS2 ERG fusion which contributes to the switch from androgen dependent to androgen independent prostate cancer (1,2).

TMPRSS2 has also been shown to play a critical role in the process of viral entry for coronaviruses and influenza viruses thru proteolytic activation of key viral glycoproteins. One pathway for SARS-CoV-2 infection, which causes the COVID-19 disease, involves binding of the SARS-CoV-2 Spike 'S' glycoprotein to the human ACE2 receptor and cleavage of S by TMPRSS2 at the cell surface to facilitate viral entry. TMPRSS2 mediates viral entry in a similar mechanism for other coronaviruses such as SARS-CoV and MERS. The broad-spectrum serine protease inhibitor, Camostat, is a TMPRSS2 inhibitor demonstrated to protect mice with lethal SARS-CoV infections (3).

References

1. Clark, J., Cooper, C. (2009) ETS gene fusions in prostate cancer. Nat Rev Urol 6, 429-439. PMID: 19657377

2. Duffy, MJ. (2014) Chapter One - PSA in Screening for Prostate Cancer: More Good than Harm or More Harm than Good? Adv Clin Chem. 66:1-23. PMID: 25344984

3. Shen LW, Mao HJ, Wu YL, Tanaka Y, Zhang W. (2017) TMPRSS2: A potential target for treatment of influenza virus and coronavirus infections. Biochimie. 142:1-10

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TMPRSS2 Antibody (NBP1-20984)(8)

Publications using NBP1-20984

• Saleem W, Ren X, Van Den Broeck W, Nauwynck H. Changes in intestinal morphology, number of mucus-producing cells and expression of coronavirus receptors APN, DPP4, ACE2 and TMPRSS2 in pigs with aging Veterinary Research 2023-04-13 [PMID: 37055856]
• Vimalanathan S, Shehata M, Sadasivam K et al. Broad Antiviral Effects of Echinacea purpurea against SARS-CoV-2 Variants of Concern and Potential Mechanism of Action Microorganisms 2022-10-29 [PMID: 36363737]
• Shook LL, Bordt EA, Meinsohn MC et al. Placental Expression of ACE2 and TMPRSS2 in Maternal Severe Acute Respiratory Syndrome Coronavirus 2 Infection: Are Placental Defenses Mediated by Fetal Sex? The Journal of Infectious Diseases 2021-12-08 [PMID: 34293137]
• Peiffer AL, Garlick JM, Wu Y et al. TMPRSS2 inhibitor discovery facilitated through an in silico and biochemical screening platform bioRxiv 2023-06-27 [PMID: 33791707]
• Samuel RM, Majd H, Richter MN et al. Androgen Signaling Regulates SARS-CoV-2 Receptor Levels and Is Associated with Severe COVID-19 Symptoms in Men Cell Stem Cell 2020-11-17 [PMID: 33232663]
• Ghazizadeh Z, Majd H, Richter M, Samuel R Androgen regulates SARS-CoV-2 receptor levels and is associated with severe COVID-19 symptoms in men bioRxiv 2020-01-01 [PMID: 32511360] (ICC/IF, Human)
• Tomlins SA, Laxman B, Dhanasekaran SM et al. Distinct classes of chromosomal rearrangements create oncogenic ETS gene fusions in prostate cancer. Nature 2007-08-02 [PMID: 17671502]
• Garlick J Innovations in Targeting Dynamic Proteins With Small Molecule Modulators Thesis 2021-01-01 (WB)

Bioinformatics

• Gene Symbol: TMPRSS2
• Entrez:
  • 7113(Human)
• Uniprot:
  • NP_005647.2(Human)