TLR3 Antibody [Alexa Fluor® 647]

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 647

TLR3 Antibody [Alexa Fluor® 647] Summary

• Specificity: Detects mouse TLR3 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human TLR3 is observed, 5% cross-reactivity with recombinant mouse (rm) TLR4 is observed, and less than 1% cross-reactivity w
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TLR3 Antibody [Alexa Fluor® 647]

• CD283 antigen
• CD283
• TLR3
• toll-like receptor 3

Background

Mouse TLR3 is a 116 kDa type I transmembrane glycoprotein that belongs to the mammalian Toll-Like Receptor family of pathogen pattern recognition molecules (1, 2). There are at least eleven mouse and ten human members that activate the innate immune system following exposure to a variety of microbial species (3). The mouse TLR3 cDNA encodes a 905 amino acid (aa) precursor that contains a 25 aa signal sequence, a 680 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 179 aa cytoplasmic region (4). The horseshoe shaped ECD (5, 6) contains 23 leucine-rich repeats, and the cytoplasmic domain contains one Toll/IL-1 receptor (TIR) domain. The ECD of mouse TLR3 shares 90%, 79%, and 77% aa sequence identity with the ECD of rat, human, and bovine TLR3, respectively. TLR3 is found in phagosomes (7), where the acidic pH enables binding of internalized double stranded RNA and mRNA from viruses, parasites, and necrotic virally-infected cells (8 - 11). Ligand binding by TLR3 induces receptor dimerization (5, 6, 8), leading to the release of inflammatory cytokines and dendritic cell maturation (9, 11 - 13). Mouse TLR3 is expressed in dendritic cells, macrophages, microglia, and astrocytes (13 - 15), and is upregulated by IFN-beta and LPS (9, 14). TLR3 expression is also induced on lung fibroblasts and epithelial cells by respiratory syncytial virus infection (12).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.