TLE3 Antibody (CL3573) - Azide and BSA Free

Staining of HeLa cells using the Anti-TLE3 monoclonal antibody) .

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line HEK 293

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line HeLa nuclear extract

Staining of human kidney shows weak to moderate nuclear positivity in a subset of cells.

Staining of human cervix shows strong nuclear immunoreactivity in epithelial cells.

Staining of human oral mucosa shows strong nuclear positivity in epithelial cells.

Staining of human skeletal muscle shows absence of immunoreactivity (negative control).

Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-TLE3 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Staining of human small intestine shows moderate nuclear immunoreactivity in glandular epithelium cells.

Staining of human tonsil shows nuclear immunoreactivity in a subset of lymphoid cells.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: CL3573
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

TLE3 Antibody (CL3573) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse TLE3 Antibody (CL3573) - Azide and BSA Free (NBP2-61621) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of Q04726, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TLE3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:10000 - 1:20000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for TLE3 Antibody (CL3573) - Azide and BSA Free

• ESG3
• ESG3Enhancer of split groucho-like protein 3
• ESGenhancer of split groucho 3
• Grg3
• HsT18976
• KIAA1547FLJ39460
• TLE3
• transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila)
• transducin-like enhancer of split 3, homolog of Drosophila E(sp1)
• transducin-like enhancer protein 3

Background

Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TLE3