TLE2 Antibody


Western Blot: TLE2 Antibody [NBP1-32158] - Sample(30 ug of whole cell lysate) A:H1299 7.5% SDS PAGE, antibody diluted at 1:1500.
Immunocytochemistry/ Immunofluorescence: TLE2 Antibody [NBP1-32158] - Paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.

Product Details

Reactivity HuSpecies Glossary
Applications WB, ICC/IF
0.75 mg/ml

Order Details

TLE2 Antibody Summary

Recombinant fragment corresponding to a region within amino acids 529 and 743 of TLE2 (Uniprot ID#Q04725)
Predicted Species
Mouse (97%), Rat (96%)
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7.0).
0.01% Thimerosal
0.75 mg/ml
Immunogen affinity purified

Reactivity Notes

Tested in human. Predicted cross-reactivity based on sequence identity: Xenopus laevis (89%), Zebrafish (89%), and Xenopus tropicalis (90%). Not yet tested in any other species.

Alternate Names for TLE2 Antibody

  • enhancer of split groucho 2
  • ESG
  • ESG2
  • ESG2Enhancer of split groucho-like protein 2
  • FLJ41188
  • GRG2
  • transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila)
  • transducin-like enhancer of split 2, homolog of Drosophila E(sp1)
  • transducin-like enhancer protein 2


The Notch signaling pathway controls cellular interactions important for the specification of a variety of fates in both invertebrates and vertebrates. Key players in the Notch pathway are the TLE genes (for transducin-like enhancer of split, also designated ESG for enhancer of split groucho), which are human homologs of the Drosophila groucho gene. Groucho is a transcriptional repressor that plays a key role in neurogenesis, segmentation and sex determination. TLEs associate with chromatin in live cells and specifically with Histone H3, but not with other core histones. Expression of the TLE genes, TLE1, TLE2, TLE3 and TLE4, correlate with immature epithelial cells that are progressing toward a terminally differentiated state, suggesting a role during epithelial differentiation. TLE1, TLE2 and TLE3 have elevated expression in cervial squamous metaplasias and carcinomas, while TLE4 is most highly expressed in the brain, particularly in the caudate nucleus. TLE1 and TLE4 contain SP and WD40 domains, through which TLE1 binds AML1 to inhibit AML1-induced transactivation of the CSF1 receptor. In early stages of cell differentiation, TLE1 is up-regulated, and TLE2 and TLE4 are down-regulated. In later stages, TLE2 and TLE4 are up-regulated, and expression of TLE1 decreases. TLE1 and TLE2 genes map in a tandem array at chromosomal location 19p13.3. The genetic loci for TLE3 and TLE4 are chromosomes 15q22 and 9, respectively.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


Isotype Controls

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Contact Information

Product PDFs


Gene Symbol TLE2

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