TLE1 Antibody

Images

 
Western Blot: TLE1 Antibody [NBP2-20651] - Wild-type (WT) and TLE1 knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TLE1 antibody [N2C1], Interna. ...read more
Immunocytochemistry/ Immunofluorescence: TLE 1 Antibody [NBP2-20651] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:500 dilution.
Western Blot: TLE 1 Antibody [NBP2-20651] - Sample (30 ug of whole cell lysate) A: 293T 7. 5% SDS PAGE gel, diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Mu, Rt, MkSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.92 mg/ml
     

Genetic Strategies

   

Order Details

TLE1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human TLE1. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Mouse (94%), Rat (93%), Monkey (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TLE1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Theoretical MW
83 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rhesus Monkey (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.92 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TLE1 Antibody

  • E(Sp1) homolog
  • enhancer of split groucho 1
  • ESG
  • ESG1Enhancer of split groucho-like protein 1
  • GRG1
  • TLE1
  • transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila)
  • transducin-like enhancer of split 1, homolog of Drosophila E(sp1)
  • transducin-like enhancer protein 1

Background

Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TLE1 Antibody (NBP2-20651) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TLE1
Entrez
Uniprot