TIRAP Antibody

Western Blot: TIRAP (TLR2 and TLR4) Antibody [NB300-990] - (1 ug/ml) staining of human liver lysate 35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Func
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TIRAP Antibody Summary

• Description: Novus Biologicals Goat TIRAP Antibody (NB300-990) is a polyclonal antibody validated for use in WB and ELISA. Anti-TIRAP Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-EGEGERDSATVSDL corresponding to C-Terminus according to NP_683708.1.
• Specificity: This protein is expected to recognise isoform b (NP_683708.1) only.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TIRAP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:128000
  • Proximity Ligation Assay
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 30 kDa band observed in human liver lysates (calculated MW of 25.3 kDa band according to NP_683708.1). Use in Proximity ligation assay reported in scientific literature (PMID: 30579042).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30579042).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TIRAP Antibody

• adapter protein wyatt
• Adaptor protein Wyatt
• MAL
• MyD88 adapter-like protein
• MyD88-2
• TIR domain-containing adapter protein
• toll/interleukin-1 receptor domain-containing adapter protein
• toll-interleukin 1 receptor (TIR) domain containing adaptor protein
• Toll-interleukin 1 receptor (TIR) domain-containing adaptor protein
• Toll-like receptor adaptor protein
• wyatt

Background

The innate immune system recognizes microbial pathogens through Toll-like receptors (TLRs), which identify pathogen-associated molecular patterns. Different TLRs recognize different pathogen-associated molecular patterns and all TLRs have a Toll-interleukin 1 receptor (TIR) domain, which is responsible for signal transduction. The protein encoded by this gene is a TIR adaptor protein involved in the TLR4 signaling pathway of the immune system. It activates NF-kappa-B, MAPK1, MAPK3 and JNK, which then results in cytokine secretion and the inflammatory response. Alternative splicing of this gene results in several transcript variants; however, not all variants have been fully described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TIRAP Antibody (NB300-990)(4)

Publications using NB300-990

• Andreakos E, Sacre SM, Smith C et al. Distinct pathways of LPS-induced NF-kappa B activation and cytokine production in human myeloid and nonmyeloid cells defined by selective utilization of MyD88 and Mal/TIRAP. Blood 2004-03-15 [PMID: 14630816]
• Smith P, Godde N, Rubio S et al. Modification of primary amines to higher order amines reduces in vivo hematological and immunotoxicity of cationic nanocarriers through TLR4 and complement pathways bioRxiv (Mouse)
• Smith P, Godde N, Rubio S et al. Modification of primary amines to higher order amines reduces in vivo hematological and immunotoxicity of cationic nanocarriers through TLR4 and complement pathways Biomaterials 2019-10-05 [PMID: 31585233]
• Blanchard EL, Loomis KH, Bhosle SM et al. Proximity ligation assays for in situ detection of innate immune activation: focus on in vitro transcribed mRNA. Mol Ther Nucleic Acids. [PMID: 30579042] (PLA, Mouse)

Bioinformatics

• Gene Symbol: TIRAP
• Entrez:
  • 114609(Human)
• Uniprot:
  • NP_683708.(Human)