TIRAP Antibody

Images

 
Western Blot: TIRAP (TLR2 and TLR4) Antibody [NB300-990] - (1 ug/ml) staining of human liver lysate 35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Func
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

TIRAP Antibody Summary

Immunogen
Peptide with sequence C-EGEGERDSATVSDL corresponding to C-Terminus according to NP_683708.1.
Specificity
This protein is expected to recognise isoform b (NP_683708.1) only.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TIRAP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:128000
  • Proximity Ligation Assay
  • Western Blot 1 - 3 ug/ml
Application Notes
WB: Approx. 30 kDa band observed in human liver lysates (calculated MW of 25.3 kDa band according to NP_683708.1). Use in Proximity ligation assay reported in scientific literature (PMID: 30579042).
Publications
Read Publications using
NB300-990 in the following applications:

  • PLA
    1 publication

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30579042).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TIRAP Antibody

  • adapter protein wyatt
  • Adaptor protein Wyatt
  • MAL
  • MyD88 adapter-like protein
  • MyD88-2
  • TIR domain-containing adapter protein
  • toll/interleukin-1 receptor domain-containing adapter protein
  • toll-interleukin 1 receptor (TIR) domain containing adaptor protein
  • Toll-interleukin 1 receptor (TIR) domain-containing adaptor protein
  • Toll-like receptor adaptor protein
  • wyatt

Background

The innate immune system recognizes microbial pathogens through Toll-like receptors (TLRs), which identify pathogen-associated molecular patterns. Different TLRs recognize different pathogen-associated molecular patterns and all TLRs have a Toll-interleukin 1 receptor (TIR) domain, which is responsible for signal transduction. The protein encoded by this gene is a TIR adaptor protein involved in the TLR4 signaling pathway of the immune system. It activates NF-kappa-B, MAPK1, MAPK3 and JNK, which then results in cytokine secretion and the inflammatory response. Alternative splicing of this gene results in several transcript variants; however, not all variants have been fully described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TIRAP Antibody (NB300-990)(4)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: PLA.


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(2)
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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol TIRAP
Entrez
Uniprot