TIPRL Antibody (OTI3D11) - Azide and BSA Free

Western Blot: TIPRL Antibody (OTI3D11) - Azide and BSA Free [NBP2-74529] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TIPRL (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TIPRL.

Immunohistochemistry: TIPRL Antibody (OTI3D11) - Azide and BSA Free [NBP2-74529] - Staining of paraffin-embedded endometrium tissue using anti-TIPRL mouse monoclonal antibody.

Flow Cytometry: TIPRL Antibody (OTI3D11) - Azide and BSA Free [NBP2-74529] - Analysis of Jurkat cells, using anti-TIPRL antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Immunohistochemistry: TIPRL Antibody (OTI3D11) - Azide and BSA Free [NBP2-74529] - Staining of paraffin-embedded prostate tissue using anti-TIPRL mouse monoclonal antibody.

Flow Cytometry: TIPRL Antibody (OTI3D11) - Azide and BSA Free [NBP2-74529] - Analysis of Hela cells, using anti-TIPRL antibody, (Red) compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC, CyTOF-ready
• Clone: OTI3D11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

TIPRL Antibody (OTI3D11) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse TIPRL Antibody (OTI3D11) - Azide and BSA Free (NBP2-02269) is a monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human TIPRL (NP_690866) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TIPRL
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 31.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for TIPRL Antibody (OTI3D11) - Azide and BSA Free

• dJ69E11.3
• MGC3794
• putative MAPK activating protein
• Putative MAPK-activating protein PM10
• TIP41, TOR signaling pathway regulator-like (S. cerevisiae)
• TIP41-like protein
• TIP41Type 2A-interacting protein
• TIPTIP41, TOR signalling pathway regulator-like (S. cerevisiae)

Background

TIPRL is an inhibitory regulator of protein phosphatase-2A (PP2A) (see PPP2CA; MIM 176915), PP4 (see PPP4C; MIM 602035), and PP6 (see PPP6C; MIM 300141) (McConnell et al., 2007 [PubMed 17384681]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TIPRL