TIMP-2 Antibody (TIMP2/8193R)

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using TIMP-2 antibody (TIMP2/8193R) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human bladder carcinoma stained with TIMP-2 antibody (TIMP2/8193R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

• Reactivity: Hu
• Applications: IHC
• Clone: TIMP2/8193R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

TIMP-2 Antibody (TIMP2/8193R) Summary

• Description: Positive Controls: Normal Placenta, Prostate or Ovarian Carcinoma.
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment of human TIMP-2 protein (exact sequence is proprietary)
• Localization: Cytoplasm. Cell surface.
• Specificity: It recognizes a protein of 21kDa, identified as tissue inhibitor of metalloproteinases-2 (TIMP-2) It is closely related to TIMP-1 and shows the highest binding affinity to both the latent (pro) and active forms of 72kDa Type IV collagenase (also known as MMP-2 or gelatinase A) It also has affinity for the active form of 92kDa Type IV collagenase (also known as MMP-9 or gelatinase B) TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TIMP2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 10mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for TIMP-2 Antibody (TIMP2/8193R)

• CSC-21Ktissue inhibitor of metalloproteinase 2
• metalloproteinase inhibitor 2
• TIMP metallopeptidase inhibitor 2
• TIMP2
• TIMP-2
• Tissue inhibitor of metalloproteinases 2

Background

The TIMP2 gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role a

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TIMP2