TIMP-4 Antibody (153934) [Unconjugated]

CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." > CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." title="TIMP-4 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 25 µg/mL Mouse Anti-Human TIMP-4 Monoclonal Antibody (Catalog # MAB974) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." />

TIMP-4 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 25 µg/mL Mouse Anti-Human TIMP-4 Monoclonal Antibody (Catalog # MAB974) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

974-TSF) was serially diluted and captured by Mouse Anti-Human TIMP‑4 Monoclonal Antibody (Catalog # MAB974) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse TIMP‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF974) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" > 974-TSF) was serially diluted and captured by Mouse Anti-Human TIMP‑4 Monoclonal Antibody (Catalog # MAB974) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse TIMP‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF974) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" title="Recombinant Human TIMP‑4 (Catalog # 974-TSF) was serially diluted and captured by Mouse Anti-Human TIMP‑4 Monoclonal Antibody (Catalog # MAB974) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse TIMP‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF974) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" />

Recombinant Human TIMP‑4 (Catalog # <a class=

• Reactivity: Hu
• Applications: WB, IHC, ELISA(Cap)
• Clone: 153934
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

TIMP-4 Antibody (153934) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human TIMP-4
  Cys30-Pro244
  Accession # Q99727
• Specificity: Detects human TIMP-4 in ELISAs and Western blots. In ELISAs, no cross-reactivity with recombinant human TIMP-1, -2, -3, or recombinant mouse TIMP-1 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TIMP4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: ELISA Detection: Human TIMP-4 Biotinylated Antibody (Catalog number BAF974)
  Standard: Recombinant Human TIMP-4 (Catalog number 974-TSF)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TIMP-4 Antibody (153934) [Unconjugated]

• metalloproteinase inhibitor 4
• TIMP metallopeptidase inhibitor 4
• TIMP4
• TIMP-4
• tissue inhibitor of metalloproteinase 4
• Tissue inhibitor of metalloproteinases 4

Background

TIMP-4 is a secreted protein that inhibits most MMPs by forming a noncovalent complex with the MMP active site. It is highly expressed in brain, heart, ovary, and skeletal muscle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TIMP-4 Antibody (MAB974)(3)

Publications using MAB974

• WW MacDonald, SS Swaminatha, JY Heo, A Castillejo, J Hsueh, BJ Liu, D Jo, A Du, H Lee, MH Kang, DJ Rhee Effect of SPARC Suppression in Mice, Perfused Human Anterior Segments, and Trabecular Meshwork Cells Investigative Ophthalmology & Visual Science, 2022-06-01;63(6):8. 2022-06-01 [PMID: 35671048] (Western Blot, Human)
• Drewlo S, Czikk M, Baczyk D, Lye S, Kingdom J Glial cell missing-1 mediates over-expression of tissue inhibitor of metalloproteinase-4 in severe pre-eclamptic placental villi. Hum. Reprod., 2011-03-15;26(5):1025-34. 2011-03-15 [PMID: 21406447] (IHC-Fr, Human)
• Villeneuve J, Block A, Le Bousse-Kerdiles MC, Lepreux S, Nurden P, Ripoche J, Nurden AT Tissue inhibitors of matrix metalloproteinases in platelets and megakaryocytes: a novel organization for these secreted proteins. Exp. Hematol., 2009-05-03;37(7):849-56. 2009-05-03 [PMID: 19410025] (ICC, Human)

Bioinformatics

• Gene Symbol: TIMP4
• Uniprot:
  • Q99727()