TIMP-2 Products

WBC023) and lysates of HeLa human cervical epithelial carcinoma cell line and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse TIMP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF971) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TIMP-2 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">

CTS008) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox" target="_blank">

HAF017). A specific band was detected for TIMP‑2 at approximately 22 kDa (as indicated) in the parental HeLa human cervical epithelial carcinoma cell line, but is not detectable in knockout HeLa human cervical epithelial carcinoma cell line. GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox" target="_blank">

902-MPNor 902-MP) activity is measured in the presence of Recombinant Human/Mouse TIMP-2 (0.143 µg/mL, Catalog # 971-TM) that has been preincubated with increasing concentrations of Goat Anti-Human TIMP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF971). The ND₅₀ is typically 2.6 µg/mL." class="big_lightbox" target="_blank">

AF971) at 5ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to decidual cells. " class="big_lightbox" target="_blank">

971-TM) was serially diluted and captured by Mouse Anti-Human TIMP‑2 Monoclonal Antibody (Catalog # MAB9711) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse TIMP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF971) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">

WBC023) and lysates of HeLa human cervical epithelial carcinoma cell line and human placenta tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TIMP-2 Monoclonal Antibody (Catalog # MAB971) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TIMP-2 at approximately 22 kDa (as indicated).This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">

CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox" target="_blank">