JMJD6/PSR Antibody

Images

 
Western Blot: JMJD6/PSR Antibody [NBP1-71693] - Analysis of JMJD6 in A431 cell lysate.
Immunocytochemistry/ Immunofluorescence: JMJD6/PSR Antibody [NBP1-71693] - JMJD6 antibody was tested at 10 ug/ml in A431 cells with FITC (green). Nuclei (Blue) were counterstained with Dapi (blue).

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Xp, ZeSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

JMJD6/PSR Antibody Summary

Immunogen
A genomic peptide made to an internal region of the human JMJD6 protein (within residues 150-350). [Swiss-Prot Q6NYC1]
Localization
Nucleus.
Predicted Species
Mouse (98%), Rat (98%), Xenopus (91%), Zebrafish (91%), Bovine (98%). Backed by our 100% Guarantee.
Clonality
Polyclonal
Host
Rabbit
Gene
JMJD6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
Application Notes
This JMJD6 antibody is useful for ICC and Western Blot, where a band is seen ~45 kDa.
Positive Control
HeLa Lysate (NB800-PC1)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-71693 in the following applications:

Reactivity Notes

Human. Immunogen has 98% identity with mouse, rat and bovine. Immunogen has 91% identity to Xenopus and Zebrafish.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 0.1% BSA, and 50% Glycerol
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for JMJD6/PSR Antibody

  • bifunctional arginine demethylase and lysyl-hydroxylase JMJD6
  • EC 1.14.11
  • EC 1.14.11.-
  • Histone arginine demethylase JMJD6
  • JmjC domain-containing protein 6
  • JMJD6
  • jumonji domain containing 6
  • Jumonji domain-containing protein 6
  • KIAA0585PSR
  • Lysyl-hydroxylase JMJD6
  • Peptide-lysine 5-dioxygenase JMJD6
  • Phosphatidylserine receptorPTDSR
  • Protein PTDSR
  • PSR
  • PTDSR
  • PTDSR1

Background

Jumonji domain containing 6 is a histone demethylating enzyme, which is active on arginine residues, but not lysines. Its activity destabilizes heterochromatin structures and activates gene expressions in euchromatin. In endothelial cells, the activity of JMJD6 is necessary for angiogenesis. If JMJD6 expression is reduced, the VEGF-receptor (Flt-1) is incorrectly spliced, leading to increased soluble Flt-1 which binds VEGF and PlGF and inhibits angiogenesis. JMJD6 also hydroxylates RNA splicing factors, and is important in embryonic differentiation. It most likely interacts specifically with single-stranded RNA, which may contribute to its early differentiation importance.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for JMJD6/PSR Antibody (NBP1-71693) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-71693:
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Images Ratings Applications Species Date Details
Western Blot JMJD6/PSR NBP1-71693
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reviewed by:
Anonymous
WB Human 01/11/2012
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Summary

ApplicationWestern Blot
Sample TestedMDA-MB-435 cell lysates
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: 5% milk in TBST, Blocking Time: 1 hour, Blocking Temp: 25 degrees Celsius

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Incubation Dilution Buffer: 5% Milk in TBST, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: donkey anti-rabbit-IgG, Secondary Ab Dilution Ratio: 1:5000

Details

Detection NotesDetection Method: ECL prime

Product General Protocols

View specific protocols for JMJD6/PSR Antibody (NBP1-71693): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for JMJD6/PSR Antibody (NBP1-71693) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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Anonymous
01/11/2012
Application: WB
Species: Human

Bioinformatics

Gene Symbol JMJD6
Entrez
Uniprot

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