Contactin-1 Antibody [Unconjugated]

Images

 
Western blot shows lysates of human brain (cortex) tissue, human brain (cerebellum) tissue, mouse brain (cerebellum) tissue, mouse brain (total) tissue, and rat brain (total) tissue. PVDF membrane was probed with ...read more
Contactin‑1 was detected in immersion fixed paraffin-embedded sections of human dorsal root ganglia using 15 µg/mL Goat Anti-Human/Mouse/Rat Contactin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Recombinant Human Contactin‑1 Fc Chimera (Catalog # 904-CN), immobilized onto a microplate, supports the adhesion of the C6 rat glioma cell line in a dose-dependent manner (orange line). Adhesion elicited by ...read more
Simple Western lane view shows lysates of human brain tissue and mouse brain tissue, loaded at 0.2 mg/mL. A specific band was detected for Contactin‑1 at approximately 158 kDa (as indicated) using 5 µg/mL of ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Contactin-1 Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Contactin-1
Glu21-Ser993
Accession # CAA79696
Specificity
Detects human Contactin-1 in direct ELISAs.  Detects human, mouse, and rat Contactin-1 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CNTN1
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Simple Western 5 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 1-5 ug/mL
Publications
Read Publications using
AF904 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Contactin-1 Antibody [Unconjugated]

  • CNTN1
  • contactin 1
  • Contactin1
  • Contactin-1
  • F11
  • F3
  • F3cam
  • Glycoprotein gp135
  • GP135
  • Neural cell surface protein F3

Background

Contactin-1 (CNTN1) is a member of the contactin subgroup within the immunoglobulin superfamily. It was originally designated contactin in human, F3 in rodents, and F11 in chicken. Other members of this family in human include Contactin-2 (TAG-1), Contactin-5 (NB-2), and Contactin-6 (NB-3). Additional family members have been described in other species. CNTN1 shares less than 50% amino acid sequence identity with the other contactins. The human and rodent CNTN1 proteins share 96% sequence identity. The 998 amino acid mature protein contains 6 Ig-like domains and 4 fibronectin type III-like domains, and is attached to the membrane by a GPI anchor. CNTN1 is differentially expressed in numerous neuronal tissues and functions in nervous system development. It associates with two other cell-surface proteins believed to participate in signal transduction, receptor protein tyrosine phosphatase beta (RPTP beta ) and Contactin-associated protein (Caspr). Reported ligands include Nr-CAM and the extracellular matrix glycoprotein, tenascin.

  1. Reid, R.A. et al. (1994) Brain Res. Mol. Brain Res. 21:1.
  2. Walsh, F.S. and P. Doherty (1991) Cell Biol. Int. Rep. 15:1151.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Contactin-1 Antibody (AF904)(11)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC, IHC, Immunoprecipitation, Western Blot.


Filter By Application
ICC
(3)
IHC
(3)
Immunoprecipitation
(1)
Western Blot
(4)
All Applications
Filter By Species
Human
(4)
Mouse
(6)
Rat
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using AF904 Applications Species
C Manso, L Querol, C Lleixà, M Poncelet, M Mekaouche, JM Vallat, I Illa, JJ Devaux Anti-Neurofascin-155 IgG4 antibodies prevent paranodal complex formation in vivo J. Clin. Invest., 2019;130(0):. 2019 [PMID: 30869655] (ICC, Human) ICC Human
A Stojic, J Bojcevski, SK Williams, R Diem, R Fairless Early Nodal and Paranodal Disruption in Autoimmune Optic Neuritis J. Neuropathol. Exp. Neurol., 2018;0(0):. 2018 [PMID: 29444299] (Western Blot, Rat) Western Blot Rat
L Montani, JA Pereira, C Norrmén, HBF Pohl, E Tinelli, M Trötzmülle, G Figlia, P Dimas, B von Nieder, R Schwager, S Jessberger, CF Semenkovic, HC Köfeler, U Suter De novo fatty acid synthesis by Schwann cells is essential for peripheral nervous system myelination J. Cell Biol., 2018;0(0):. 2018 [PMID: 29434029] (IHC, Mouse) IHC Mouse
L Querol, AM Siles, R Alba-Rovir, A Jáuregui, J Devaux, C Faivre-Sar, J Araque, R Rojas-Garc, J Diaz-Maner, E Cortés-Vic, G Nogales-Ga, M Navas-Madr, E Gallardo, I Illa Antibodies against peripheral nerve antigens in chronic inflammatory demyelinating polyradiculoneuropathy Sci Rep, 2017;7(1):14411. 2017 [PMID: 29089585] (ICC, Human) ICC Human
Pierre-Marie Martin Schwannomin-interacting protein 1 isoform IQCJ-SCHIP1 is a multi-partner ankyrin and spectrin binding protein involved in the organization of nodes of Ranvier J. Biol. Chem, 2016;0(0):. 2016 [PMID: 27979964] (IHC, Mouse) IHC Mouse
Colakoglu G, Bergstrom-Tyrberg U, Berglund E, Ranscht B Contactin-1 regulates myelination and nodal/paranodal domain organization in the central nervous system. Proc Natl Acad Sci U S A, 2014;111(3):E394-403. 2014 [PMID: 24385581] (Western Blot, Mouse) Western Blot Mouse
Tian N, Leshchyns'ka I, Welch J, Diakowski W, Yang H, Schachner M, Sytnyk V Lipid raft-dependent endocytosis of close homolog of adhesion molecule L1 (CHL1) promotes neuritogenesis. J Biol Chem, 2012;287(53):44447-63. 2012 [PMID: 23144456] (Western Blot, Mouse) Western Blot Mouse
Dours-Zimmermann MT, Maurer K, Rauch U, Stoffel W, Fassler R, Zimmermann DR Versican V2 assembles the extracellular matrix surrounding the nodes of ranvier in the CNS. J. Neurosci., 2009;29(24):7731-42. 2009 [PMID: 19535585] (IHC, Mouse) IHC Mouse
Lieberoth A, Splittstoesser F, Katagihallimath N, Jakovcevski I, Loers G, Ranscht B, Karagogeos D, Schachner M, Kleene R Lewis(x) and alpha2,3-sialyl glycans and their receptors TAG-1, Contactin, and L1 mediate CD24-dependent neurite outgrowth. J. Neurosci., 2009;29(20):6677-90. 2009 [PMID: 19458237] (Immunoprecipitation, Mouse) Immunoprecipitation Mouse
Su JL, Yang CY, Shih JY, Wei LH, Hsieh CY, Jeng YM, Wang MY, Yang PC, Kuo ML Knockdown of contactin-1 expression suppresses invasion and metastasis of lung adenocarcinoma. Cancer Res., 2006;66(5):2553-61. 2006 [PMID: 16510572] (Western Blot, Human) Western Blot Human
Show All 11 Publications.

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Secondary Antibodies

 

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Other Available Formats

Biotin BAF904

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Bioinformatics

Gene Symbol CNTN1
Uniprot