Contactin-1 Antibody [Unconjugated]

Contactin‑1 was detected in immersion fixed paraffin-embedded sections of human dorsal root ganglia using 15 µg/mL Goat Anti-Human/Mouse/Rat Contactin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF904) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western blot shows lysates of human brain (cortex) tissue, human brain (cerebellum) tissue, mouse brain (cerebellum) tissue, mouse brain (total) tissue, and rat brain (total) tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat Contactin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF904) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Contactin‑1 at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of human brain tissue and mouse brain tissue, loaded at 0.2 mg/mL. A specific band was detected for Contactin‑1 at approximately 158 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat Contactin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF904) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Recombinant Human Contactin‑1 Fc Chimera (Catalog # 904-CN), immobilized onto a microplate, supports the adhesion of the C6 rat glioma cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human Contactin‑1 Fc Chimera (2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse/RatContactin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF904). The ND50 is typically 1-5 µg/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Contactin-1 Antibody [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Contactin-1
  Glu21-Ser993
  Accession # CAA79696
• Specificity: Detects human Contactin-1 in direct ELISAs. Detects human, mouse, and rat Contactin-1 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CNTN1
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 1-5 ug/mL
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Contactin-1 Antibody [Unconjugated]

• CNTN1
• contactin 1
• Contactin1
• Contactin-1
• F11
• F3
• F3cam
• Glycoprotein gp135
• GP135
• Neural cell surface protein F3

Background

Contactin-1 (CNTN1) is a member of the contactin subgroup within the immunoglobulin superfamily. It was originally designated contactin in human, F3 in rodents, and F11 in chicken. Other members of this family in human include Contactin-2 (TAG-1), Contactin-5 (NB-2), and Contactin-6 (NB-3). Additional family members have been described in other species. CNTN1 shares less than 50% amino acid sequence identity with the other contactins. The human and rodent CNTN1 proteins share 96% sequence identity. The 998 amino acid mature protein contains 6 Ig-like domains and 4 fibronectin type III-like domains, and is attached to the membrane by a GPI anchor. CNTN1 is differentially expressed in numerous neuronal tissues and functions in nervous system development. It associates with two other cell-surface proteins believed to participate in signal transduction, receptor protein tyrosine phosphatase beta (RPTP beta ) and Contactin-associated protein (Caspr). Reported ligands include Nr-CAM and the extracellular matrix glycoprotein, tenascin.

1. Reid, R.A. et al. (1994) Brain Res. Mol. Brain Res. 21:1.
2. Walsh, F.S. and P. Doherty (1991) Cell Biol. Int. Rep. 15:1151.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Contactin-1 Antibody (AF904)(14)

Publications using AF904

• Y Ogawa, BC Lim, S George, JA Oses-Priet, JM Rasband, Y Eshed-Eise, S Nair, F Boato, E Peles, AL Burlingame, LV Aelst, MN Rasband Antibody-directed extracellular proximity biotinylation reveals Contactin-1 regulates axo-axonic innervation of axon initial segments bioRxiv : the preprint server for biology, 2023-03-06;0(0):. 2023-03-06 [PMID: 36945454] (ICC, Rat)
• R Kuwayama, K Suzuki, J Nakamura, E Aizawa, Y Yoshioka, M Ikawa, S Nabatame, KI Inoue, Y Shimmyo, K Ozono, T Kinoshita, Y Murakami Establishment of mouse model of inherited PIGO deficiency and therapeutic potential of AAV-based gene therapy Nature Communications, 2022-06-03;13(1):3107. 2022-06-03 [PMID: 35661110] (Western Blot, Mouse)
• KJ Chang, I Agrawal, A Vainshtein, WY Ho, W Xin, G Tucker-Kel, K Susuki, E Peles, SC Ling, JR Chan TDP-43 maximizes nerve conduction velocity by repressing a cryptic exon for paranodal junction assembly in Schwann cells Elife, 2021-03-10;10(0):. 2021-03-10 [PMID: 33689679] (IHC, Mouse)
• C Manso, L Querol, C Lleixà, M Poncelet, M Mekaouche, JM Vallat, I Illa, JJ Devaux Anti-Neurofascin-155 IgG4 antibodies prevent paranodal complex formation in vivo J. Clin. Invest., 2019-03-14;130(0):. 2019-03-14 [PMID: 30869655] (ICC, Human)
• A Stojic, J Bojcevski, SK Williams, R Diem, R Fairless Early Nodal and Paranodal Disruption in Autoimmune Optic Neuritis J. Neuropathol. Exp. Neurol., 2018-05-01;0(0):. 2018-05-01 [PMID: 29444299] (Western Blot, Rat)
• L Montani, JA Pereira, C Norrmén, HBF Pohl, E Tinelli, M Trötzmülle, G Figlia, P Dimas, B von Nieder, R Schwager, S Jessberger, CF Semenkovic, HC Köfeler, U Suter De novo fatty acid synthesis by Schwann cells is essential for peripheral nervous system myelination J. Cell Biol., 2018-02-06;0(0):. 2018-02-06 [PMID: 29434029] (IHC, Mouse)
• L Querol, AM Siles, R Alba-Rovir, A Jáuregui, J Devaux, C Faivre-Sar, J Araque, R Rojas-Garc, J Diaz-Maner, E Cortés-Vic, G Nogales-Ga, M Navas-Madr, E Gallardo, I Illa Antibodies against peripheral nerve antigens in chronic inflammatory demyelinating polyradiculoneuropathy Sci Rep, 2017-10-31;7(1):14411. 2017-10-31 [PMID: 29089585] (ICC, Human)
• Pierre-Marie Martin Schwannomin-interacting protein 1 isoform IQCJ-SCHIP1 is a multi-partner ankyrin and spectrin binding protein involved in the organization of nodes of Ranvier J. Biol. Chem, 2016-12-15;0(0):. 2016-12-15 [PMID: 27979964] (IHC, Mouse)
• Colakoglu G, Bergstrom-Tyrberg U, Berglund E, Ranscht B Contactin-1 regulates myelination and nodal/paranodal domain organization in the central nervous system. Proc Natl Acad Sci U S A, 2014-01-02;111(3):E394-403. 2014-01-02 [PMID: 24385581] (Western Blot, Mouse)
• Tian N, Leshchyns'ka I, Welch J, Diakowski W, Yang H, Schachner M, Sytnyk V Lipid raft-dependent endocytosis of close homolog of adhesion molecule L1 (CHL1) promotes neuritogenesis. J Biol Chem, 2012-11-09;287(53):44447-63. 2012-11-09 [PMID: 23144456] (Western Blot, Mouse)
• Dours-Zimmermann MT, Maurer K, Rauch U, Stoffel W, Fassler R, Zimmermann DR Versican V2 assembles the extracellular matrix surrounding the nodes of ranvier in the CNS. J. Neurosci., 2009-06-17;29(24):7731-42. 2009-06-17 [PMID: 19535585] (IHC, Mouse)
• Lieberoth A, Splittstoesser F, Katagihallimath N, Jakovcevski I, Loers G, Ranscht B, Karagogeos D, Schachner M, Kleene R Lewis(x) and alpha2,3-sialyl glycans and their receptors TAG-1, Contactin, and L1 mediate CD24-dependent neurite outgrowth. J. Neurosci., 2009-05-20;29(20):6677-90. 2009-05-20 [PMID: 19458237] (ICC, Immunoprecipitation, Neutralization, Mouse)
• Su JL, Yang CY, Shih JY, Wei LH, Hsieh CY, Jeng YM, Wang MY, Yang PC, Kuo ML Knockdown of contactin-1 expression suppresses invasion and metastasis of lung adenocarcinoma. Cancer Res., 2006-03-01;66(5):2553-61. 2006-03-01 [PMID: 16510572] (Immunoprecipitation, Western Blot, Human)
• Shah BS, Rush AM, Liu S, Tyrrell L, Black JA, Dib-Hajj SD, Waxman SG Contactin associates with sodium channel Nav1.3 in native tissues and increases channel density at the cell surface. J. Neurosci., 2004-08-18;24(33):7387-99. 2004-08-18 [PMID: 15317864] (ICC, Western Blot, Human, Rat)

Bioinformatics

• Gene Symbol: CNTN1
• Uniprot:
  • CAA79696()