TIM-2 Antibody (RMT2-14) - Chimeric - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: TIM-2 Antibody (RMT2-14) - Chimeric [NBP3-09035] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of RMT2-14 (NBP3-09035) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09035, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

• Reactivity: Mu
• Applications: WB, Flow, ICC/IF, IHC, B/N, Func
• Clone: RMT2-14
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

TIM-2 Antibody (RMT2-14) - Chimeric - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit TIM-2 Antibody (RMT2-14) - Chimeric - Azide and BSA Free (NBP3-09035) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was raised by immunising Sprague Dawley rats with TIM-2-Ig consisting of the extracellular domain (aa 1-230 of mouse TIM-2) and the Fc portion of mouse IgG2a, emulsified in complete Freund's adjuvant and then fusing lymph node cells from the immunized rats with P3U1 myeloma cells.
• Isotype: IgG Lambda
• Clonality: Monoclonal
• Host: Rabbit
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • Flow Cytometry
  • Functional Assay
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot
• Application Notes: This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
  
  This antibody has been used in various FACS analyses, for instance, to evaluate the expression of TIM family members on mouse mast cells (Nakae et al, 2007) and to block the H-ferritin binding to TIM-2/L5178Y cells (Kawamoto et al, 2011). This antibody has been used in FACS and immunohistochemistry analyses to show that TIM-2 is expressed on mouse fetal hepatocytes (Watanabe et al, 2007). This antibody has also been used in Western Blots and for blocking experiments in ferritin binding analysis, which suggests that Tim-2 is the H-ferritin receptor on oligodendrocytes (Todorich et al, 2008). In addition, this antibody has been used in collagen-induced arthritis (CIA) models to determine whether TIM-2 contributes to the development of T helper (Th) 1 or Th17 cells and joint inflammation (Kawamoto et al, 2011).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for TIM-2 Antibody (RMT2-14) - Chimeric - Azide and BSA Free

• G9D3
• T-cell immunoglobulin and mucin domain-containing protein 2
• T-cell immunoglobulin mucin receptor 2
• T-cell membrane protein 2
• TIM2
• TIM-2
• TIMD-2

Background

Tim-2 is a transmembrane protein known as T cell immunoglobulin and mucin domain containing protein-2. It is expressed on activated T cells and preferentially on differentiated Th2 cells. Sema4A is the ligand of Tim-2. A blockade of Tim-2/Tim-2 ligand interaction results in T cell hyperproliferation and Th2 cytokine production. Tim-2 plays a critical role in the development of atopic disease and other Th2-biased immune responses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.