Semaphorin 4D/CD100 Antibody (5H6E3) - BSA Free

Western Blot: Semaphorin 4D/CD100 Antibody (5H6E3) [NBP2-61663] - Analysis using CD100 mAb against human CD100 (AA: extra 590-734) recombinant protein. (Expected MW is 43.2 kDa)

Immunohistochemistry: Semaphorin 4D/CD100 Antibody (5H6E3) [NBP2-61663] - Analysis of paraffin-embedded bladder cancer tissues using CD100 mouse mAb with DAB staining.

Flow Cytometry: Semaphorin 4D/CD100 Antibody (5H6E3) [NBP2-61663] - Analysis of Ramos cells using CD100 mouse mAb (green) and negative control (red).

Western Blot: Semaphorin 4D/CD100 Antibody (5H6E3) [NBP2-61663] - Analysis using CD100 mAb against HEK293 (1) and CD100 (AA: extra 590-734)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemistry: Semaphorin 4D/CD100 Antibody (5H6E3) [NBP2-61663] - Analysis of paraffin-embedded cervical cancer tissues using CD100 mouse mAb with DAB staining.

Flow Cytometry: Semaphorin 4D/CD100 Antibody (5H6E3) [NBP2-61663] - Analysis of K562 cells using CD100 mouse mAb (green) and negative control (red).

ELISA: Semaphorin 4D/CD100 Antibody (5H6E3) [NBP2-61663] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

• Reactivity: Hu
• Applications: WB, ELISA, Flow, IHC
• Clone: 5H6E3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Semaphorin 4D/CD100 Antibody (5H6E3) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human Semaphorin 4D/CD100 (AA: extra 590-734) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SEMA4D
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:100-1:2000
• Theoretical MW: 96.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Semaphorin 4D/CD100 Antibody (5H6E3) - BSA Free

• A8
• BB18
• CD100 antigen
• CD100
• CD100M-sema-G
• COLL-4
• FLJ33485
• FLJ34282
• FLJ39737
• FLJ46484
• immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, 4D
• MGC169138
• MGC169141
• M-sema-G
• sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4D
• SEMA4D
• SEMAJ
• Semaphorin 4D
• semaphorin-4D

Background

SEMA4D (Semaphorin 4D) is a Protein Coding gene. Diseases associated with SEMA4D include Hemorrhagic Fever With Renal Syndrome. Among its related pathways are Guidance Cues and Growth Cone Motility and Developmental Biology. GO annotations related to this gene include receptor binding and transmembrane signaling receptor activity. An important paralog of this gene is SEMA4B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SEMA4D