CD72 Antibody (BU40) - BSA Free

• Reactivity: Hu, RM
• Applications: WB, Flow, IHC, IP, CyTOF-ready
• Clone: BU40
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

CD72 Antibody (BU40) - BSA Free Summary

• Additional Information: Clone BU40 was used by HLDA to establish CD designation.
• Immunogen: HF B1 plasmacytoid cell line. Considered to be late B like.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD72
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:50 - 1:100
  • Immunohistochemistry 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:100 - 1:2000
• Application Notes: This antibody is Cytof ready.
• Control:

  CD72 Overexpression Lysate

Reactivity Notes

Use in Rhesus Macaque reported in scientific literature (PMID:32023469).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CD72 Antibody (BU40) - BSA Free

• CD72 antigenB-cell differentiation antigen CD72
• CD72 molecule
• CD72
• CD72b
• Ly-19.2
• Ly-32.2
• Lyb-2
• LYB2lyb-2

Background

CD72 antigen is a member of the type II integral membrane glycoproteins which includes other related cell surface molecules such as the asialoglycoprotein receptors, CD23 and the Kupffer cell receptor. The function of CD72 is unknown but the exposure of B cells to CD72 antibodies activates a variety of signaling pathways and can induce MHC class II expression and B cell proliferation. CD72 antigen is expressed on all cells of B cell lineage with the exception of plasma cells and weakly on human tissue macrophages.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD72 Antibody (NB100-64350)(1)

Publication using NB100-64350

• Han Q, Bradley T et al Neonatal Rhesus Macaques Have Distinct Immune Cell Transcriptional Profiles following HIV Envelope Immunization Cell Rep 2020-02-06 [PMID: 32023469] (FLOW, Rhesus Macaque)
  
  Details:
  Citation using the PerCP version of this antibody.

FAQs for CD72 Antibody (NB100-64350). (Showing 1 - 1 of 1 FAQ).

1. We purchased a product from you, CD72 Antibody (Bu40) cat# NB100-64350. Could you confirm the Ig class, please?
   • The Ig Class is IgG2a.

Bioinformatics

• Gene Symbol: CD72
• Entrez:
  • 971(Human)
• Uniprot:
  • P21854()