TIM-1/KIM-1/HAVCR Antibody [Unconjugated]

TIM-1/KIM-1/HAVCR was detected in perfusion fixed frozen sections of rat kidney using 15 µg/mL Rat TIM-1/KIM-1/HAVCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3689) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Immunohistochemical analysis of kidneys in LETO and OLETF rats.Representative immunohistochemical images of glomerular, tubular and tubulointerstitial lesions in each group, 12 weeks after starting to feed a Paigen diet. Scale bar, 100 μm. KIM-1, kidney injury molecule-1; MRP8, myeloid-related protein 8; NOX2, NADPH oxidase 2; OPN, osteopontin; TLR4, toll-like receptor 4; TNF-alpha , tumor necrosis factor-alpha . Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0143979), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

TIM-1/KIM-1/HAVCR Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant rat TIM-1/KIM-1/HAVCR
  Ser18-Val238
  Accession # O54947
• Specificity: Detects rat TIM-1/KIM-1/HAVCR in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) TIM-1 is observed, and less than 1% cross-reactivity with recombinant human TIM-1, recombinant rat TIM-2, rmTIM-3, rmTIM-4, rmTIM-6, and rmTIM-7 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HAVCR1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is covered by one or more of the following US Patents 7,300,652; 7,041,290; 6,664,385 and other US and foreign patents pending or issued.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TIM-1/KIM-1/HAVCR Antibody [Unconjugated]

• CD365
• HAVCR1
• HAVCR-1
• HAVCRT cell immunoglobin domain and mucin domain protein 1
• hepatitis A virus cellular receptor 1
• Kidney injury molecule 1
• KIM1
• KIM-1
• T-cell immunoglobulin and mucin domain-containing protein 1
• TIM1
• TIM-1
• TIM-1TIM
• TIM1TIMD-1
• TIMD1T-cell membrane protein 1

Background

KIM-1 (Kidney-injury molecule-1; also TIM-1 and HAVCR) is a 50-80 kDa, variably glycosylated, type I transmembrane glycoprotein that is a member of the TIM family of immunoglobulin superfamily molecules. This gene family is involved in the regulation of Th1 and Th2-cell-mediated immunity. In mouse, there are eight known TIM/KIM genes (# 1-8) vs. only three genes in human (# 1, 3, 4). It is unknown if the rat genome exactly parallels that of mouse. Rat KIM-1 is synthesized as a 307 amino acid (aa) precursor that contains a 21 aa signal sequence, a 214 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 51 aa cytoplasmic domain. The ECD contains one V-type Ig-like domain and a mucin region characterized by multiple Thr-Ser-Pro motifs. The mucin region may undergo extensive O-linked glycosylation. The mouse KIM-1 gene is highly polymorphic and this may be reflected in rat. In human, TIM-1 is known to circulate as a soluble form. It undergoes constitutive cleavage by an undefined MMP, releasing an 85 kDa soluble molecule. A similar process has now been described in rat. The ECD of rat KIM-1 is 50% and 81% aa identical to human and mouse KIM-1 ECD, respectively. The only two reported ligands for KIM-1 are TIM-4 and the hepatitis A virus. However, others are believed to exist, and based on the ligand for TIM-3, one might be an S-type lectin. KIM-1 is found on CD4⁺ T cells and proximal renal tubular cells. KIM-1 ligation induces T cell proliferation and promotes cytokine production. In particular, it induces IL-4 production, and requires the KIM-1 cytoplasmic tyrosine phosphorylation motif. Alternatively, KIM-1 activation of TIM-4 induces cell cycle arrest.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TIM-1/KIM-1/HAVCR Antibody (AF3689)(39)

Publications using AF3689

• Hunter, RW;Sun, J;Palmer, T;Czopek, A;Nespoux, J;Bailey, MA;Dhaun, N;Buck, AH;Dear, JW; SLAMseq reveals potential transfer of RNA from liver to kidney in the mouse Nature communications 2025-08-11 [PMID: 40789845] (Immunohistochemistry, Transgenic Mouse)
• Nelson, P;Dugbartey, GJ;McFarlane, L;McLeod, P;Major, S;Jiang, J;O'Neil, C;Haig, A;Sener, A; Effect of Sodium Thiosulfate Pre-Treatment on Renal Ischemia-Reperfusion Injury in Kidney Transplantation International journal of molecular sciences 2024-09-02 [PMID: 39273476] (Immunohistochemistry, Rat)
• J Huang, E Caliskan G, SK Gholami, KL Gawelek, RN Mitchell, LH Pojoga, JR Romero, GH Williams, GK Adler Effects of Mineralocorticoid Receptor Blockade and Statins on Kidney Injury Marker 1 (KIM-1) in Female Rats Receiving L-NAME and Angiotensin II International Journal of Molecular Sciences, 2023-03-30;24(7):. 3/30/2023 [PMID: 37047470]
• I Nagayama, K Takayanagi, H Hasegawa, A Maeshima Tubule-Derived Follistatin Is Increased in the Urine of Rats with Renal Ischemia and Reflects the Severity of Acute Tubular Damage Cells, 2023-03-04;12(5):. 3/4/2023 [PMID: 36899937]
• Zeltzin Alejandra Ceja-Galicia, Fernando Enrique García-Arroyo, Omar Emiliano Aparicio-Trejo, Mohammed El-Hafidi, Guillermo Gonzaga-Sánchez, Juan Carlos León-Contreras et al. Therapeutic Effect of Curcumin on 5/6Nx Hypertriglyceridemia: Association with the Improvement of Renal Mitochondrial beta -Oxidation and Lipid Metabolism in Kidney and Liver Antioxidants (Basel) 11/6/2022 [PMID: 36358567]
• MM Rinschen, O Palygin, A El-Meanawy, X Domingo-Al, A Palermo, LV Dissanayak, D Golosova, MA Schafroth, C Guijas, F Demir, J Jaegers, ML Gliozzi, J Xue, M Hoehne, T Benzing, BP Kok, E Saez, M Bleich, N Himmerkus, OA Weisz, BF Cravatt, M Krüger, HP Benton, G Siuzdak, A Staruschen Accelerated lysine metabolism conveys kidney protection in salt-sensitive hypertension Nature Communications, 2022-07-14;13(1):4099. 2022-07-14 [PMID: 35835746] (IHC, Rat)
• Ya Yue, Jui-Ning Yeh, John Y. Chiang, Pei-Hsun Sung, Yi-Ling Chen, Fanna Liu et al. Intrarenal arterial administration of human umbilical cord-derived mesenchymal stem cells effectively preserved the residual renal function of diabetic kidney disease in rat Stem Cell Research & Therapy 12/1/2022 [PMID: 35526048]
• JY Lim, WW Jung, W Kim, KS Moon, D Sul Nephrotoxicity evaluation and proteomic analysis in kidneys of rats exposed to thioacetamide Scientific Reports, 2022-04-27;12(1):6837. 2022-04-27 [PMID: 35477741] (Western Blot, Rat)
• Patrick Krohn, Laura Rita Rega, Marianne Harvent, Beatrice Paola Festa, Anna Taranta, Alessandro Luciani et al. Multisystem involvement, defective lysosomes and impaired autophagy in a novel rat model of nephropathic cystinosis Human Molecular Genetics 7/7/2022 [PMID: 35137071]
• A Al-Brakati, KF Alsharif, KJ Alzahrani, S Kabrah, O Al-Amer, AA Oyouni, OA Habotta, MS Lokman, AA Bauomy, RB Kassab, AE Abdel Mone Using Green Biosynthesized Lycopene-Coated Selenium Nanoparticles to Rescue Renal Damage in Glycerol-Induced Acute Kidney Injury in Rats International Journal of Nanomedicine, 2021-06-29;16(0):4335-4349. 2021-06-29 [PMID: 34234429] (ELISA Capture, Rat)
• MOROKI T, MATSUO S, HATAKEYAMA H et al. Databases for technical aspects of immunohistochemistry-2021 update Journal of Toxicologic Pathology 2/24/2021 [PMID: 33976473]
• A Kuno, Y Kimura, M Mizuno, H Oshima, T Sato, N Moniwa, M Tanaka, T Yano, M Tanno, T Miki, T Miura Empagliflozin attenuates acute kidney injury after myocardial infarction in diabetic rats Sci Rep, 2020-04-29;10(1):7238. 2020-04-29 [PMID: 32350374] (IHC, Rat)
• S Kalyan, M Bazett, HP Sham, M Bosiljcic, B Luk, S Dhanji, AM Costa, SWY Wong, MG Netea, DW Mullins, H Gunn Distinct inactivated bacterial-based immune modulators vary in their therapeutic efficacies for treating disease based on the organ site of pathology Sci Rep, 2020-04-03;10(1):5901. 2020-04-03 [PMID: 32246043] (IHC, Rat)
• M Kitada, Y Ogura, I Monno, J Xu, D Koya Methionine abrogates the renoprotective effect of a low-protein diet against diabetic kidney disease in obese rats with type 2 diabetes Aging (Albany NY), 2020-03-06;12(5):4489-4505. 2020-03-06 [PMID: 32145700] (IHC, Rat)
• Aya Imafuku, Masatoshi Oka, Yoei Miyabe, Sachiko Sekiya, Kosaku Nitta, Tatsuya Shimizu Rat Mesenchymal Stromal Cell Sheets Suppress Renal Fibrosis via Microvascular Protection Stem Cells Translational Medicine 12/1/2019 [PMID: 31675167]
• Y Kimura, A Kuno, M Tanno, T Sato, K Ohno, S Shibata, K Nakata, H Sugawara, K Abe, Y Igaki, T Yano, T Miki, T Miura Canagliflozin, an SGLT2 inhibitor, normalizes renal susceptibility to type 1 cardiorenal syndrome through reduction of renal oxidative stress in diabetic rats J Diabetes Investig, 2019-02-25;0(0):. 2019-02-25 [PMID: 30663266] (IHC-P, Rat)
• YX He, TT Diao, SM Song, CC Wang, Y Wang, CL Zhou, YB Bai, SS Yu, X Mi, XY Yang, QJ Wei, B Li Wnt4 is significantly upregulated during the early phases of cisplatin-induced acute kidney injury Sci Rep, 2018-07-12;8(1):10555. 2018-07-12 [PMID: 30002385] (IHC, Rat)
• PC Vogelaar, M Roorda, EL de Vrij, MC Houwertjes, M Goris, H Bouma, AC van der Gr, G Krenning, RH Henning The 6-hydroxychromanol derivative SUL-109 ameliorates renal injury after deep hypothermia and rewarming in rats Nephrol. Dial. Transplant., 2018-12-01;0(0):. 2018-12-01 [PMID: 29660027] (IHC-Fr, Western Blot, Rat)
• W Ding, K Yousefi, S Goncalves, BJ Goldstein, AL Sabater, A Kloosterbo, P Ritter, G Lambert, AJ Mendez, LA Shehadeh Osteopontin deficiency ameliorates Alport pathology by preventing tubular metabolic deficits JCI Insight, 2018-03-22;3(6):. 2018-03-22 [PMID: 29563333] (IHC-Fr, Mouse)
• K Toyoda, Y Suzuki, K Muta, T Masuyama, K Kakimoto, A Kobayashi, T Shoda, S Sugai High fructose diet feeding accelerates diabetic nephropathy in Spontaneously Diabetic Torii (SDT) rats J Toxicol Sci, 2018-01-01;43(1):45-58. 2018-01-01 [PMID: 29415951] (IHC-P, Rat)
• CH Chen, BC Cheng, KH Chen, PL Shao, PH Sung, HJ Chiang, CC Yang, KC Lin, CK Sun, JJ Sheu, HW Chang, MS Lee, HK Yip Combination therapy of exendin-4 and allogenic adipose-derived mesenchymal stem cell preserved renal function in a chronic kidney disease and sepsis syndrome setting in rats Oncotarget, 2017-10-10;8(59):100002-100020. 2017-10-10 [PMID: 29245956] (IHC-P, Rat)
• Kouhei Ohno, Atsushi Kuno, Hiromichi Murase, Shingo Muratsubaki, Takayuki Miki, Masaya Tanno et al. Diabetes increases the susceptibility to acute kidney injury after myocardial infarction through augmented activation of renal Toll-like receptors in rats American Journal of Physiology-Heart and Circulatory Physiology 12/1/2017 [PMID: 28822965]
• Jing Ying Ma, Sandra Snook, Sheryl Garrovillo, Charles Johnson, David La An Immunohistochemical Investigation of Renal Phospholipidosis and Toxicity in Rats International Journal of Toxicology 9/1/2017 [PMID: 28820006]
• X Chen, A Cobbs, J George, A Chima, F Tuyishime, X Zhao Endocytosis of Albumin Induces Matrix Metalloproteinase-9 by Activating the ERK Signaling Pathway in Renal Tubule Epithelial Cells Int J Mol Sci, 2017-08-12;18(8):. 2017-08-12 [PMID: 28805677] (IHC, Rat)
• KH Chen, CH Chen, CG Wallace, YT Chen, CC Yang, PH Sung, HJ Chiang, YL Chen, S Chua, HK Yip, JT Cheng Combined therapy with melatonin and exendin-4 effectively attenuated the deterioration of renal function in rat cardiorenal syndrome Am J Transl Res, 2017-02-15;9(2):214-229. 2017-02-15 [PMID: 28337255] (IHC-P, Rat)
• Raimo Tuuminen, Annukka Jouppila, Dan Salvail, Charles-E. Laurent, Marie-Claude Benoit, Simo Syrjälä et al. Dual antiplatelet and anticoagulant APAC prevents experimental ischemia–reperfusion-induced acute kidney injury Clinical and Experimental Nephrology 2017-06-01 [PMID: 27405618] (Immunohistochemistry, Rat)
• Raimo Tuuminen, Annukka Jouppila, Dan Salvail, Charles-E. Laurent, Marie-Claude Benoit, Simo Syrjälä et al. Dual antiplatelet and anticoagulant APAC prevents experimental ischemia–reperfusion-induced acute kidney injury Clinical and Experimental Nephrology 6/1/2017 [PMID: 27405618]
• Stefan Gauer, Anja Urbschat, Norbert Gretz, Sigrid C. Hoffmann, Bettina Kränzlin, Helmut Geiger et al. Kidney Injury Molecule-1 Is Specifically Expressed in Cystically-Transformed Proximal Tubules of the PKD/Mhm (cy/+) Rat Model of Polycystic Kidney Disease International Journal of Molecular Sciences 5/24/2016 [PMID: 27231899]
• Carine M Boustany-Kari, Paul C Harrison, Hongxing Chen, Kathleen A Lincoln, Hu Sheng Qian, Holly Clifford et al. A Soluble Guanylate Cyclase Activator Inhibits the Progression of Diabetic Nephropathy in the ZSF1 Rat Journal of Pharmacology and Experimental Therapeutics 2/11/2016 [PMID: 26729306]
• Nozako M, Koyama T, Nagano C, Sato M, Matsumoto S, Mitani K, Yasufuku R, Kohashi M, Yoshikawa T An Atherogenic Paigen-Diet Aggravates Nephropathy in Type 2 Diabetic OLETF Rats. PLoS ONE, 2015-11-25;10(11):e0143979. 2015-11-25 [PMID: 26606054] (IHC-P, Rat)
• Xueying Zhao, Chen Jiang, Rebecca Olufade, Dong Liu, Nerimiah Emmett Kidney Injury Molecule-1 Enhances Endocytosis of Albumin in Renal Proximal Tubular Cells Journal of Cellular Physiology 4/1/2016 [PMID: 26332568]
• José Sereno, Sara Nunes, Paulo Rodrigues-Santos, Helena Vala, Petronila Rocha-Pereira, João Fernandes et al. Conversion to Sirolimus Ameliorates Cyclosporine-Induced Nephropathy in the Rat: Focus on Serum, Urine, Gene, and Protein Renal Expression Biomarkers BioMed Research International 5/19/2014 [PMID: 24971338]
• Zhao X, Zhang Y, Leander M, Li L, Wang G, Emmett N Altered expression profile of renal alpha(1D)-adrenergic receptor in diabetes and its modulation by PPAR agonists. J Diabetes Res, 2014-03-17;2014(0):725634. 2014-03-17 [PMID: 24772448] (IHC, Rat)
• Lekawanvijit S, Kumfu S, Wang B, Manabe M, Nishijima F, Kelly D, Krum H, Kompa A The uremic toxin adsorbent AST-120 abrogates cardiorenal injury following myocardial infarction. PLoS ONE, 2013-12-13;8(12):e83687. 2013-12-13 [PMID: 24349545] (IHC, Rat)
• Katherine Shea, Sharron Stewart, Rodney Rouse Assessment standards: comparing histopathology, digital image analysis, and stereology for early detection of experimental cisplatin-induced kidney injury in rats Toxicologic Pathology 8/1/2014 [PMID: 24201815]
• Mitzi Paola Santoyo-Sánchez, José Pedraza-Chaverri, Eduardo Molina-Jijón, Laura Arreola-Mendoza, Rafael Rodríguez-Muñoz, Olivier Christophe Barbier Impaired endocytosis in proximal tubule from subchronic exposure to cadmium involves angiotensin II type 1 and cubilin receptors BMC Nephrology 12/1/2013 [PMID: 24093454]
• Petra Vinken, Sofie Starckx, Erio Barale-Thomas, Adriana Looszova, Manisha Sonee, Nick Goeminne et al. Tissue Kim-1 and Urinary Clusterin as Early Indicators of Cisplatin-Induced Acute Kidney Injury in Rats Toxicologic Pathology 10/1/2012 [PMID: 22581811]
• Ferreira L, Quiros Y, Sancho-Martinez SM, Garcia-Sanchez O, Raposo C, Lopez-Novoa JM, Gonzalez-Buitrago JM, Lopez-Hernandez FJ Urinary levels of regenerating islet-derived protein III beta and gelsolin differentiate gentamicin from cisplatin-induced acute kidney injury in rats. Kidney Int., 2010-10-27;79(5):518-28. 2010-10-27 [PMID: 20980976] (Western Blot, Rat)
• Quiros Y, Ferreira L, Sancho-Martinez SM, Gonzalez-Buitrago JM, Lopez-Novoa JM, Lopez-Hernandez FJ Sub-nephrotoxic doses of gentamicin predispose animals to developing acute kidney injury and to excrete ganglioside M2 activator protein. Kidney Int., 2010-08-18;78(10):1006-15. 2010-08-18 [PMID: 20720524] (Western Blot, Rat)

Bioinformatics

• Gene Symbol: HAVCR1
• Uniprot:
  • O54947()