TIM-1/KIM-1/HAVCR Antibody [Unconjugated]

TIM-1/KIM-1/HAVCR was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human TIM-1/KIM-1/HAVCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1750) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Formalin-fixed paraffin-embedded tissue sections of human kidney were probed for TIM1 mRNA (ACD RNAScope Probe, catalog #452148; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using goat anti-human TIM1 polyclonal antibody (R&D Systems catalog # AF1750) at 1ug/mL with 1 hour incubation at room temperature followed by incubation with anti-goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to tubules.

• Reactivity: Hu
• Applications: WB, IHC, Dual ISH-IHC, ELISA(Cap)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

TIM-1/KIM-1/HAVCR Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human TIM‑1/KIM-1/HAVCR (R&D Systems, Catalog # 1750-TM)
  Ser21-Thr288
  Accession # Q96D42
• Specificity: Detects human TIM‑1/KIM-1/HAVCR in ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HAVCR1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Dual RNAscope ISH-IHC 5-15 ug/mL
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 1-15 ug/mL
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Human TIM-1/KIM-1/HAVCR Antibody (Catalog # AF1750)
  ELISA Detection: Human TIM-1/KIM-1/HAVCR Biotinylated Antibody (Catalog # BAF1750)
  Standard: Recombinant Human TIM-1/KIM-1/HAVCR (Catalog # 1750-TM)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is covered by one or more of the following US Patents 7,300,652; 7,041,290; 6,664,385 and other US and foreign patents pending or issued.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TIM-1/KIM-1/HAVCR Antibody [Unconjugated]

• CD365
• HAVCR1
• HAVCR-1
• HAVCRT cell immunoglobin domain and mucin domain protein 1
• hepatitis A virus cellular receptor 1
• Kidney injury molecule 1
• KIM1
• KIM-1
• T-cell immunoglobulin and mucin domain-containing protein 1
• TIM1
• TIM-1
• TIM-1TIM
• TIM1TIMD-1
• TIMD1T-cell membrane protein 1

Background

TIM-1 (T cell-immunoglobulin-mucin; also known as KIM-1 and HAVCR) is a 100 kDa, type I transmembrane glycoprotein member of the TIM family of immunoglobulin superfamily molecules (1-3). This gene family is involved in the regulation of Th1 and Th2-cell-mediated immunity. Human TIM-1 is synthesized as a 359 amino acid (aa) precursor that contains a 20 aa signal sequence, a 270 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 48 aa cytoplasmic domain (4-6). The ECD contains oneV-type Ig-like domain and a mucin region characterized by multiple PTTTTL motifs. The mucin region undergoes extensive O-linked glycosylation. The TIM-1 gene is highly polymorphic and undergoes alternate splicing (1). For instance, the presence of a six aa sequence (MTTTVP) at position #137 of the mature molecule is associated with protection from atopy in people with a history of hepatitis A (7, 8). There are two cytoplasmic alternate splice forms of TIM‑1. One is a long (359 aa) kidney form termed TIM-1b, and one is a short (334 aa) liver form termed TIM-1a. Both are identical through the first 323 aa of their precursors. TIM-1b contains a tyrosine phosphorylation motif that is not present in 1a (6). TIM-1 is also known to circulate as a soluble form. Constitutive cleavage by an undefined MMP (possibly ADAM33) releases an 85-90 kDa soluble molecule (6). The ECD of human TIM-1 is 50% and 43% aa identical to mouse and canine TIM-1 ECD, respectively. The only two reported ligands for TIM-1 are TIM-4 and the hepatitis A virus (4, 9). However, others are believed to exist, and based on the ligand for TIM-3, one may well be an S-type lectin (10). TIM-1 ligation induces T cell proliferation and promotes cytokine production (1, 10).

1. Meyers, J.H. et al. (2005) Trends Mol. Med. 11:1471.
2. Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454.
3. Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.
4. Feigelstock, D. et al. (1998) J. Virol. 72:6621.
5. Ichimura, T. et al. (1998) J. Biol. Chem. 273:4135.
6. Bailly, V. et al. (2002) J. Biol. Chem. 277:39739.
7. Umetsu, D.T. et al. (2005) J. Pediatr. Gastroenterol. Nutr. 40:S43.
8. Gao, P-S. et al. (2005) J. Allergy Clin. Immunol. 115:982.
9. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
10. Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TIM-1/KIM-1/HAVCR Antibody (AF1750)(39)

Publications using AF1750

• Messingham, KN;Richards, PT;Fleck, A;Patel, RA;Djurkovic, M;Elliff, J;Connell, S;Crowe, TP;Munoz Gonzalez, J;Gourronc, F;Dillard, JA;Davey, RA;Klingelhutz, A;Shtanko, O;Maury, W; Multiple cell types support productive infection and dynamic translocation of infectious Ebola virus to the surface of human skin Science advances 2025-01-03 [PMID: 39742475] (Flow Cytometry, Human)
• Oeyen, M;Heymann, CJF;Jacquemyn, M;Daelemans, D;Schols, D; The Role of TIM-1 and CD300a in Zika Virus Infection Investigated with Cell-Based Electrical Impedance Biosensors 2024-07-25 [PMID: 39194591] (Western Blot, Human)
• Schnell, J;Miao, Z;Achieng, M;Fausto, CC;Wang, V;Kuyper, F;Thornton, ME;Grubbs, B;Kim, J;Lindström, NO; Stepwise developmental mimicry generates proximal-biased kidney organoids bioRxiv : the preprint server for biology 2024-07-02 [PMID: 39005387] (Immunohistochemistry, Mouse)
• Liu, M;Zhang, C;Gong, X;Zhang, T;Lian, MM;Chew, EGY;Cardilla, A;Suzuki, K;Wang, H;Yuan, Y;Li, Y;Naik, MY;Wang, Y;Zhou, B;Soon, WZ;Aizawa, E;Li, P;Low, JH;Tandiono, M;Montagud, E;Moya-Rull, D;Rodriguez Esteban, C;Luque, Y;Fang, M;Khor, CC;Montserrat, N;Campistol, JM;Izpisua Belmonte, JC;Foo, JN;Xia, Y; Kidney organoid models reveal cilium-autophagy metabolic axis as a therapeutic target for PKD both in vitro and in vivo Cell stem cell 2024-01-04 [PMID: 38181751] (Immunohistochemistry, Xenograft)
• Liang, Z;Pan, J;Xie, S;Yang, X;Cao, R; Interaction between hTIM-1 and Envelope Protein Is Important for JEV Infection Viruses 2023-07-21 [PMID: 37515282] (ICC, Human)
• Susa K, Kobayashi K, Galichon P et al. ATP/ADP biosensor organoids for drug nephrotoxicity assessment Frontiers in cell and developmental biology 3/2/2023 [PMID: 36936695]
• Judith Mary Reyes Ballista, Kerri L. Miazgowicz, Marissa D. Acciani, Ariana R. Jimenez, Ryan S. Belloli, Katherine E. Havranek et al. Chikungunya virus entry and infectivity is primarily facilitated through cell line dependent attachment factors in mammalian and mosquito cells Frontiers in Cell and Developmental Biology 1/20/2023 [PMID: 36743418]
• T Hattori, T Saito, H Miyamoto, M Kajihara, M Igarashi, A Takada Single Nucleotide Variants of the Human TIM-1 IgV Domain with Reduced Ability to Promote Viral Entry into Cells Viruses, 2022-09-26;14(10):. 2022-09-26 [PMID: 36298679] (Western Blot, Human)
• Jessica M. Vanslambrouck, Sean B. Wilson, Ker Sin Tan, Ella Groenewegen, Rajeev Rudraraju, Jessica Neil et al. Enhanced metanephric specification to functional proximal tubule enables toxicity screening and infectious disease modelling in kidney organoids Nature Communications 10/8/2022 [PMID: 36209212]
• K Hiratsuka, T Miyoshi, KT Kroll, NR Gupta, MT Valerius, T Ferrante, M Yamashita, JA Lewis, R Morizane Organoid-on-a-chip model of human ARPKD reveals mechanosensing pathomechanisms for drug discovery Science Advances, 2022-09-21;8(38):eabq0866. 2022-09-21 [PMID: 36129975] (IHC, Human)
• Yi Zheng, Robin Zhexuan Yan, Shiyu Sun, Mutsumi Kobayashi, Lifeng Xiang, Ran Yang et al. Single-cell analysis of embryoids reveals lineage diversification roadmaps of early human development Cell Stem Cell 9/1/2022 [PMID: 36055194]
• Jessica M. Vanslambrouck, Sean B. Wilson, Ker Sin Tan, Ella Groenewegen, Rajeev Rudraraju, Jessica Neil et al. Enhanced metanephric specification to functional proximal tubule enables toxicity screening and infectious disease modelling in kidney organoids bioRxiv 4/15/2023 [PMID: 35665006]
• Gupta N, Matsumoto T, Hiratsuka K et al. Modeling injury and repair in kidney organoids reveals that homologous recombination governs tubular intrinsic repair Science Translational Medicine 3/2/2022 [PMID: 35235339]
• Gupta N, Matsumoto T, Hiratsuka K et al. Modeling injury and repair in kidney organoids reveals that homologous recombination governs tubular intrinsic repair Science Translational Medicine 2022-03-02 [PMID: 35235339]
• Przepiorski A, Vanichapol T, Espiritu E Et al. Modeling oxidative injury response in human kidney organoids Stem Cell Res Ther 2/22/2022 [PMID: 35189973]
• X Zhang, C Liang, H Wang, Z Guo, H Rong, J Pan, W Li, R Pei, X Chen, Z Zhang, XE Zhang, Z Cui T-Cell Immunoglobulin and Mucin Domain 1 (TIM-1) Is a Functional Entry Factor for Tick-Borne Encephalitis Virus MBio, 2022-01-25;0(0):e0286021. 2022-01-25 [PMID: 35073759] (ICC, Human)
• Xiaohong Xiang, Jiefu Zhu, Gang Zhang, Zhengwei Ma, Man J. Livingston, Zheng Dong Proximal Tubule p53 in Cold Storage/Transplantation-Associated Kidney Injury and Renal Graft Dysfunction Frontiers in Medicine 10/22/2021 [PMID: 34746182]
• J Kirui, Y Abidine, A Lenman, K Islam, YD Gwon, L Lasswitz, M Evander, M Bally, G Gerold The Phosphatidylserine Receptor TIM-1 Enhances Authentic Chikungunya Virus Cell Entry Cells, 2021-07-20;10(7):. 2021-07-20 [PMID: 34359995] (Flow Cytometry, Human)
• Insa M Schmidt, Anand Srivastava, Venkata Sabbisetti, Gearoid M McMahon, Jiang He, Jing Chen et al. Plasma Kidney Injury Molecule 1 in CKD: Findings From the Boston Kidney Biopsy Cohort and CRIC Studies American Journal of Kidney Diseases 2/1/2022 [PMID: 34175376]
• Wenxin Xu, Mäneka Puligandla, Brian Halbert, Naomi B. Haas, Keith T. Flaherty, Robert G. Uzzo et al. Plasma KIM-1 Is Associated with Recurrence Risk after Nephrectomy for Localized Renal Cell Carcinoma: A Trial of the ECOG-ACRIN Research Group (E2805) Clinical Cancer Research 6/15/2021 [PMID: 33832947]
• K Uchimura, H Wu, Y Yoshimura, BD Humphreys Human Pluripotent Stem Cell-Derived Kidney Organoids with Improved Collecting Duct Maturation and Injury Modeling Cell Reports, 2020-12-15;33(11):108514. 2020-12-15 [PMID: 33326782] (IHC, Human)
• K Kongmanas, N Punyadee, K Wasuworawo, A Songjaeng, T Prommool, Y Pewkliang, S Manocheewa, S Thiemmeca, K Sa-Ngiamsu, C Puttikhunt, KF Faull, S Hongeng, P Avirutnan Immortalized stem cell-derived hepatocyte-like cells: An alternative model for studying dengue pathogenesis and therapy PLoS Negl Trop Dis, 2020-11-20;14(11):e0008835. 2020-11-20 [PMID: 33216752] (Flow Cytometry, Human)
• Ruofan Wang, Germán G. Gornalusse, Yeseul Kim, Urvashi Pandey, Florian Hladik, Lucia Vojtech Potent Restriction of Sexual Zika Virus Infection by the Lipid Fraction of Extracellular Vesicles in Semen Frontiers in Microbiology 9/29/2020 [PMID: 33133043]
• Jiefu Zhu, Gang Zhang, Zhixia Song, Xiaohong Xiang, Shaoqun Shu, Zhiwen Liu et al. Protein Kinase C-δ Mediates Kidney Tubular Injury in Cold Storage–Associated Kidney Transplantation Journal of the American Society of Nephrology 5/1/2020 [PMID: 32291286]
• A Herrador, C Fedeli, E Radulovic, KP Campbell, H Moreno, G Gerold, S Kunz Dynamic Dystroglycan Complexes Mediate Cell Entry of Lassa Virus MBio, 2019-03-26;10(2):. 2019-03-26 [PMID: 30914516] (Neutralization, Human)
• Garreta E, Prado P, Tarantino C et al. Fine tuning the extracellular environment accelerates the derivation of kidney organoids from human pluripotent stem cells Nature Materials 4/1/2019 [PMID: 30778227]
• Garreta E, Prado P, Tarantino C et al. Fine tuning the extracellular environment accelerates the derivation of kidney organoids from human pluripotent stem cells Nature Materials 2019-04-01 [PMID: 30778227]
• J Niu, Y Jiang, H Xu, C Zhao, G Zhou, P Chen, R Cao TIM-1 Promotes Japanese Encephalitis Virus Entry and Infection Viruses, 2018-11-14;10(11):. 2018-11-14 [PMID: 30441759] (ICC, Human)
• Balajikarthick Subramanian, Oguzhan Kaya, Martin R. Pollak, Gang Yao, Jing Zhou Guided tissue organization and disease modeling in a kidney tubule array Biomaterials 11/1/2018 [PMID: 30189357]
• Rachel B. Brouillette, Elisabeth K. Phillips, Radhika Patel, Wadie Mahauad-Fernandez, Sven Moller-Tank, Kai J. Rogers et al. TIM-1 Mediates Dystroglycan-Independent Entry of Lassa Virus Journal of Virology 8/15/2018 [PMID: 29875238]
• A Kumar, J Jovel, J Lopez-Oroz, D Limonta, AM Airo, S Hou, I Stryapunin, C Fibke, RB Moore, TC Hobman Human Sertoli cells support high levels of Zika virus replication and persistence Sci Rep, 2018-04-03;8(1):5477. 2018-04-03 [PMID: 29615760] (Neutralization, Human)
• A Das, A Hirai-Yuki, O González-L, B Rhein, S Moller-Tan, R Brouillett, L Hensley, I Misumi, W Lovell, JM Cullen, JK Whitmire, W Maury, SM Lemon TIM1 (HAVCR1) Is Not Essential for Cellular Entry of Either Quasi-enveloped or Naked Hepatitis A Virions MBio, 2017-09-05;8(5):. 9/5/2017 [PMID: 28874468]
• Y Jin, X Shao, B Sun, C Miao, Z Li, Y Shi Urinary kidney injury molecule?1 as an early diagnostic biomarker of obstructive acute kidney injury and development of a rapid detection method Mol Med Rep, 2017-01-05;0(0):. 2017-01-05 [PMID: 28075469] (Immunochromatography, Rat)
• Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells Nat Protoc, 2016-12-22;12(1):195-207. 2016-12-22 [PMID: 28005067] (IHC, Human)
• Chung H, Pellegrini K, Chung J, Wanigasuriya K, Jayawardene I, Lee K, Lee H, Vaidya V, Weissleder R Nanoparticle Detection of Urinary Markers for Point-of-Care Diagnosis of Kidney Injury. PLoS ONE, 2015-07-17;10(7):e0133417. 2015-07-17 [PMID: 26186708] (ELISA Development, Human)
• Makoto Kuroda, Daisuke Fujikura, Asuka Nanbo, Andrea Marzi, Osamu Noyori, Masahiro Kajihara et al. Interaction between TIM-1 and NPC1 Is Important for Cellular Entry of Ebola Virus Journal of Virology 6/15/2015 [PMID: 25855742]
• Venkata S. Sabbisetti, Sushrut S. Waikar, Daniel J. Antoine, Adam Smiles, Chang Wang, Abinaya Ravisankar et al. Blood Kidney Injury Molecule-1 Is a Biomarker of Acute and Chronic Kidney Injury and Predicts Progression to ESRD in Type I Diabetes Journal of the American Society of Nephrology 10/1/2014 [PMID: 24904085]
• Venkata S. Sabbisetti, Sushrut S. Waikar, Daniel J. Antoine, Adam Smiles, Chang Wang, Abinaya Ravisankar et al. Blood Kidney Injury Molecule-1 Is a Biomarker of Acute and Chronic Kidney Injury and Predicts Progression to ESRD in Type I Diabetes Journal of the American Society of Nephrology 2014-10-01 [PMID: 24904085] (Western Blot, ELISA Capture, Human)
• Moller-Tank S, Albritton L, Rennert P, Maury W Characterizing functional domains for TIM-mediated enveloped virus entry. J Virol, 2014-04-02;88(12):6702-13. 2014-04-02 [PMID: 24696470] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: HAVCR1
• Uniprot:
  • Q96D42()