Tight Junction Protein 1 Antibody

Images

 
Western Blot: Tight Junction Protein 1 Antibody [NBP2-21051] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody ...read more
Immunocytochemistry/ Immunofluorescence: Tight Junction Protein 1 Antibody [NBP2-21051] - Tight Junction Protein 1 Antibody Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: TJP1 protein stained by ZO-1 ...read more
Western Blot: Tight Junction Protein 1 Antibody [NBP2-21051] - Tight Junction Protein 1 Antibody Sample (30 ug of whole cell lysate) A: HCT116 5% SDS PAGE gel, diluted at 1:5000.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.53 mg/ml
     

Genetic Strategies

   

Order Details

Tight Junction Protein 1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human ZO-1. The exact sequence is proprietary.
Localization
Cell membrane; Peripheral membrane protein; Cytoplasmic side; Cell junction; tight junction
Marker
Intercellular Junctions/Tight Junction Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TJP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Theoretical MW
187 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.53 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Tight Junction Protein 1 Antibody

  • DKFZp686M05161
  • MGC133289
  • tight junction protein 1 (zona occludens 1)
  • Tight junction protein 1
  • tight junction protein ZO-1
  • TJP1
  • ZO1
  • ZO-1
  • zona occludens 1
  • Zona occludens protein 1
  • zonula occludens 1 protein
  • Zonula occludens protein 1

Background

This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Tight Junction Protein 1 Antibody (NBP2-21051). (Showing 1 - 2 of 2 FAQ).

  1. The Molecular Weight of it should be 187KD,why it seems nearly 250KDa in our image. Can you so kind to help him and me?
    • Our Tight Junction Protein 1 antibody with catalogue number NBP2-21051 was raised to a recombinant protein fragment corresponding to a region within amino acids 556 and 939 of ZO-1 (Uniprot ID #Q07157). According to the Uniprot listing, this protein has a theoretical molecular weight of 195kDa, but undergoes multiple post-translational modifications which would increase the weight further. Since this protein is very large, it will not run exactly at the listed molecular weight, however we are confident that NBP2-21051 is detecting a band which corresponds to Tight Junction Protein 1.
  2. Can this antibody be used for rat sample by WB and ICC/IF. It does not mention in the product datasheet.
    • Our Tight Junction Protein 1 antibody with catalogue number NBP2-21051 has been validated for Western blotting and ICC/IF with samples from human, and the antibody is predicted to react with the mouse protein - mouse is covered by our 100% quality guarantee. This antibody has not yet been tested with samples from rat.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Tight Junction Protein 1 Antibody (NBP2-21051)

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Blogs on Tight Junction Protein 1.

Zonula Occludens (ZO) the Junction Scaffolding Proteins
The Zonula Occludens (ZO) proteins 1,2 and 3, also known as tight junction proteins, are peripheral proteins localizing at junctional sites(1). ZO proteins are known to be scaffolding proteins recruiting various types of proteins to the cytoplasmic...  Read full blog post.

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Bioinformatics

Gene Symbol TJP1
Uniprot