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![Western Blot: Tight Junction Protein 1 Antibody (BLR092G) [NBP2-80141] - Detection of human TJP1/ZO-1 by WB of A-549, K-562, HEK293T, MCF-7, Jurkat, HeLa, LNCaP, and RKO lysate. Antibody: Rabbit anti-TJP1/ZO-1 recombinant monoclonal [BLR092G] (NBP2-80141). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Lower Panel: Rabbit anti-COPB2 antibody.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-BLR092G-Western-Blot-NBP2-80141-img0006.jpg "Western Blot: Tight Junction Protein 1 Antibody (BLR092G) [NBP2-80141] - Detection of human TJP1/ZO-1 by WB of A-549, K-562, HEK293T, MCF-7, Jurkat, HeLa, LNCaP, and RKO lysate. Antibody: Rabbit anti-TJP1/ZO-1 recombinant monoclonal [BLR092G] (NBP2-80141). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Lower Panel: Rabbit anti-COPB2 antibody.")
![Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody (BLR092G) [NBP2-80141] - Rabbit anti-ZO-1 recombinant monoclonal antibody [BLR092G]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-BLR092G-Immunocytochemistry-Immunofluorescence-NBP2-80141-img0007.jpg)
![Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody (BLR092G) [NBP2-80141] - Rabbit anti-ZO-1 recombinant monoclonal antibody [BLR092G]. Secondary: HRPconjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-BLR092G-Immunohistochemistry-Paraffin-NBP2-80141-img0009.jpg)
![Western Blot: Tight Junction Protein 1 Antibody (BLR092G) [NBP2-80141] - Detection of mouse TJP1/ZO-1 by WB of mIMCD-3, RenCa, NIH 3T3, TCMK-1, and C2C12 lysate. Antibody: Rabbit anti-TJP1/ZO-1 recombinant monoclonal [BLR092G] (A700-092). Secondary: HRP-conjugated goat anti-rabbit IgG (NBP2-80141). Lower Panel: Rabbit anti-COPB2 antibody.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-BLR092G-Western-Blot-NBP2-80141-img0005.jpg)
![Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody (BLR092G) [NBP2-80141] - Rabbit anti-ZO-1 recombinant monoclonal antibody [BLR092G]. Secondary: HRPconjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-BLR092G-Immunohistochemistry-Paraffin-NBP2-80141-img0008.jpg)
![Immunoprecipitation: Tight Junction Protein 1 Antibody (BLR092G) [NBP2-80141] - Detection of human TJP1/ZO-1 by WB immunoprecipitates from A-549 lysate. Antibodies: Rabbit anti-TJP1/ZO-1 recombinant monoclonal [BLR092G] (NBP2-80141) and another rabbit anti-TJP1/ZO-1.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-BLR092G-Immunoprecipitation-NBP2-80141-img0001.jpg)
![Western Blot: Tight Junction Protein 1 Antibody (HL1185) - Azide and BSA Free [NBP3-25774] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [HL1185] (NBP3-25774) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25774_rabbit-tight-junction-protein-1-mab-hl1185-azide-bsa-free-212024936270.jpg "Western Blot: Tight Junction Protein 1 Antibody (HL1185) - Azide and BSA Free [NBP3-25774] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [HL1185] (NBP3-25774) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: Tight Junction Protein 1 Antibody (HL1185) - Azide and BSA Free [NBP3-25774] - Wild-type (WT) and TJP1 knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [HL1185] (NBP3-25774) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25774_rabbit-tight-junction-protein-1-mab-hl1185-azide-bsa-free-2120249374943.jpg)
![Western Blot: Tight Junction Protein 1 Antibody (HL1185) - Azide and BSA Free [NBP3-25774] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [HL1185] (NBP3-25774) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25774_rabbit-tight-junction-protein-1-mab-hl1185-azide-bsa-free-2120249415937.jpg)
![Western Blot: Tight Junction Protein 1 Antibody (HL1185) - Azide and BSA Free [NBP3-25774] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [HL1185] (NBP3-25774) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25774_rabbit-tight-junction-protein-1-mab-hl1185-azide-bsa-free-212024940839.jpg)
![Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody (HL1185) - Azide and BSA Free [NBP3-25774] - ZO-1 antibody [HL1185] detects ZO-1 protein at cell membrane by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZO-1 stained by ZO-1 antibody [HL1185] (NBP3-25774) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25774_rabbit-tight-junction-protein-1-mab-hl1185-azide-bsa-free-2120249432869.jpg)
![Western Blot: Tight Junction Protein 1 Antibody (HL1185) - Azide and BSA Free [NBP3-25774] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [HL1185] (NBP3-25774) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25774_rabbit-tight-junction-protein-1-mab-hl1185-azide-bsa-free-2120249371525.jpg)
![ELISA: Human Tight Junction Protein 1 ELISA Kit (Colorimetric) [NBP3-40250] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-40250_human-tight-junction-protein-1-elisa-kit-colorimetric-2110202415393797.png "ELISA: Human Tight Junction Protein 1 ELISA Kit (Colorimetric) [NBP3-40250] - Standard Curve Reference")
![ELISA: Human Tight Junction Protein 1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40249] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-40249_human-tight-junction-protein-1-ready-to-use-elisa-kit-2110202415343935.png "ELISA: Human Tight Junction Protein 1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40249] - Standard Curve Reference")
![Western Blot: TJAP1 Antibody [NBP2-38152] - Analysis in control (vector only transfected HEK293T lysate) and TJAP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/TJAP1-Antibody-Western-Blot-NBP2-38152-img0006.jpg "Western Blot: TJAP1 Antibody [NBP2-38152] - Analysis in control (vector only transfected HEK293T lysate) and TJAP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: TJAP1 Antibody [NBP2-38152] - Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.](http://images.novusbio.com/fullsize/TJAP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38152-img0005.jpg)
![Immunohistochemistry-Paraffin: TJAP1 Antibody [NBP2-38152] - Staining of human placenta shows strong cytoplasmic positivity with a granular pattern in trophoblastic cells.](http://images.novusbio.com/fullsize/TJAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-38152-img0004.jpg)
![Immunohistochemistry-Paraffin: TJAP1 Antibody [NBP1-87273] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/antibody/nbp1-87273_rabbit-polyclonal-tjap1-antibody-immunohistochemistry-paraffin-59202472910..jpg "Immunohistochemistry-Paraffin: TJAP1 Antibody [NBP1-87273] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.")
![Immunohistochemistry-Paraffin: TJAP1 Antibody [NBP1-87273] - Staining of human lymph node shows moderate cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/antibody/nbp1-87273_rabbit-polyclonal-tjap1-antibody-immunohistochemistry-paraffin-59202473021..jpg)
![Immunohistochemistry-Paraffin: TJAP1 Antibody [NBP1-87273] - staining of human endometrium shows moderate cytoplasmic-membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-87273_rabbit-polyclonal-tjap1-antibody-immunohistochemistry-paraffin-5920248534..jpg)
![Immunohistochemistry-Paraffin: TJAP1 Antibody [NBP1-87273] - Staining of human placenta shows strong cytoplasmic granular positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/antibody/nbp1-87273_rabbit-polyclonal-tjap1-antibody-immunohistochemistry-paraffin-5920248632..jpg)
![Western Blot: TJAP1 Antibody [NBP1-53200] - Titration: 0.2-1 ug/ml, Positive Control: Human Placenta.](http://images.novusbio.com/fullsize/TJAP1-Antibody-Western-Blot-NBP1-53200-img0003.jpg "Western Blot: TJAP1 Antibody [NBP1-53200] - Titration: 0.2-1 ug/ml, Positive Control: Human Placenta.")
![Western Blot: TJAP1 Antibody - BSA Free [NBP3-45234] - Human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with (TJAP1 antibody) at dilution of 1:1500](http://images.novusbio.com/fullsize/nbp3-45234_rabbit-tjap1-pab-bsa-free-284202512371386.jpg "Western Blot: TJAP1 Antibody - BSA Free [NBP3-45234] - Human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with (TJAP1 antibody) at dilution of 1:1500")