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![Western Blot: Tight Junction Protein 1 Antibody (BLR092G) [NBP2-80141] - Detection of human TJP1/ZO-1 by WB of A-549, K-562, HEK293T, MCF-7, Jurkat, HeLa, LNCaP, and RKO lysate. Antibody: Rabbit anti-TJP1/ZO-1 recombinant monoclonal [BLR092G] (NBP2-80141). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Lower Panel: Rabbit anti-COPB2 antibody.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-BLR092G-Western-Blot-NBP2-80141-img0006.jpg "Western Blot: Tight Junction Protein 1 Antibody (BLR092G) [NBP2-80141] - Detection of human TJP1/ZO-1 by WB of A-549, K-562, HEK293T, MCF-7, Jurkat, HeLa, LNCaP, and RKO lysate. Antibody: Rabbit anti-TJP1/ZO-1 recombinant monoclonal [BLR092G] (NBP2-80141). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Lower Panel: Rabbit anti-COPB2 antibody.")
![Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody (BLR092G) [NBP2-80141] - Rabbit anti-ZO-1 recombinant monoclonal antibody [BLR092G]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-BLR092G-Immunocytochemistry-Immunofluorescence-NBP2-80141-img0007.jpg)
![Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody (BLR092G) [NBP2-80141] - Rabbit anti-ZO-1 recombinant monoclonal antibody [BLR092G]. Secondary: HRPconjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-BLR092G-Immunohistochemistry-Paraffin-NBP2-80141-img0009.jpg)
![Western Blot: Tight Junction Protein 1 Antibody (BLR092G) [NBP2-80141] - Detection of mouse TJP1/ZO-1 by WB of mIMCD-3, RenCa, NIH 3T3, TCMK-1, and C2C12 lysate. Antibody: Rabbit anti-TJP1/ZO-1 recombinant monoclonal [BLR092G] (A700-092). Secondary: HRP-conjugated goat anti-rabbit IgG (NBP2-80141). Lower Panel: Rabbit anti-COPB2 antibody.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-BLR092G-Western-Blot-NBP2-80141-img0005.jpg)
![Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody (BLR092G) [NBP2-80141] - Rabbit anti-ZO-1 recombinant monoclonal antibody [BLR092G]. Secondary: HRPconjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-BLR092G-Immunohistochemistry-Paraffin-NBP2-80141-img0008.jpg)
![Immunoprecipitation: Tight Junction Protein 1 Antibody (BLR092G) [NBP2-80141] - Detection of human TJP1/ZO-1 by WB immunoprecipitates from A-549 lysate. Antibodies: Rabbit anti-TJP1/ZO-1 recombinant monoclonal [BLR092G] (NBP2-80141) and another rabbit anti-TJP1/ZO-1.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-BLR092G-Immunoprecipitation-NBP2-80141-img0001.jpg)
![Western Blot: Tight Junction Protein 1 Antibody (HL1185) - Azide and BSA Free [NBP3-25774] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [HL1185] (NBP3-25774) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25774_rabbit-tight-junction-protein-1-mab-hl1185-azide-bsa-free-212024936270.jpg "Western Blot: Tight Junction Protein 1 Antibody (HL1185) - Azide and BSA Free [NBP3-25774] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [HL1185] (NBP3-25774) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: Tight Junction Protein 1 Antibody (HL1185) - Azide and BSA Free [NBP3-25774] - Wild-type (WT) and TJP1 knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [HL1185] (NBP3-25774) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25774_rabbit-tight-junction-protein-1-mab-hl1185-azide-bsa-free-2120249374943.jpg)
![Western Blot: Tight Junction Protein 1 Antibody (HL1185) - Azide and BSA Free [NBP3-25774] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [HL1185] (NBP3-25774) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25774_rabbit-tight-junction-protein-1-mab-hl1185-azide-bsa-free-2120249415937.jpg)
![Western Blot: Tight Junction Protein 1 Antibody (HL1185) - Azide and BSA Free [NBP3-25774] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [HL1185] (NBP3-25774) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25774_rabbit-tight-junction-protein-1-mab-hl1185-azide-bsa-free-212024940839.jpg)
![Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody (HL1185) - Azide and BSA Free [NBP3-25774] - ZO-1 antibody [HL1185] detects ZO-1 protein at cell membrane by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZO-1 stained by ZO-1 antibody [HL1185] (NBP3-25774) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25774_rabbit-tight-junction-protein-1-mab-hl1185-azide-bsa-free-2120249432869.jpg)
![Western Blot: Tight Junction Protein 1 Antibody (HL1185) - Azide and BSA Free [NBP3-25774] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [HL1185] (NBP3-25774) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25774_rabbit-tight-junction-protein-1-mab-hl1185-azide-bsa-free-2120249371525.jpg)
![ELISA: Human Tight Junction Protein 1 ELISA Kit (Colorimetric) [NBP3-40250] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-40250_human-tight-junction-protein-1-elisa-kit-colorimetric-2110202415393797.png "ELISA: Human Tight Junction Protein 1 ELISA Kit (Colorimetric) [NBP3-40250] - Standard Curve Reference")
![ELISA: Human Tight Junction Protein 1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40249] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-40249_human-tight-junction-protein-1-ready-to-use-elisa-kit-2110202415343935.png "ELISA: Human Tight Junction Protein 1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40249] - Standard Curve Reference")