Tight Junction Protein 1 Antibody

Images

 
Western Blot: Tight Junction Protein 1 Antibody [NBP1-85046] - Analysis of Tight Junction Protein 1 in RIPA whole cell lysates of mouse primary choroid plexus epithelial cells using anti-Tight Junction Protein 1 ...read more
Immunocytochemistry/ Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85046] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & cell junctions.
Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85046] - Staining of human kidney shows distinct membranous and cytoplasmic positivity in cells of glomeruli.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Tight Junction Protein 1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:ASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGR
Marker
Intercellular Junctions/Tight Junction Marker
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Predicted Species
Rat (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TJP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Positive Control
Brain Cerebral Meninges Lysate (NB820-60575)
Control Peptide
Tight Junction Protein 1 Protein (NBP1-85046PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-85046 in the following applications:

Publications
Read Publications using NBP1-85046.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Tight Junction Protein 1 Antibody

  • DKFZp686M05161
  • MGC133289
  • tight junction protein 1 (zona occludens 1)
  • Tight junction protein 1
  • tight junction protein ZO-1
  • TJP1
  • ZO1
  • ZO-1
  • zona occludens 1
  • Zona occludens protein 1
  • zonula occludens 1 protein
  • Zonula occludens protein 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Tight Junction Protein 1 Antibody (NBP1-85046)(2)

Review for Tight Junction Protein 1 Antibody (NBP1-85046) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-85046:
Filter by Applications
WB
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
Western Blot Tight Junction Protein 1 NBP1-85046
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reviewed by:
Anonymous
WB Mouse 08/26/2015
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Summary

ApplicationWestern Blot
Sample TestedRIPA whole cell lysates of mouse primary choroid plexus epithelial cells
SpeciesMouse

Blocking

Blocking DetailsLicoR blocking buffer, 1h, Room temperature

Primary Anitbody

Dilution Ratio1:1000, overnight at 4 degrees, in Licor odyssey blocking buffer

Secondary Antibody

Secondary DescriptionLiCor Goat anti-rabbit IR680
Secondary Concentration1:15000

Details

Detection NotesLiCoR Odyssey CFx scanner

Comments

CommentsBand at expected size/molecular weight of 220KDa.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Tight Junction Protein 1 Antibody (NBP1-85046) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Tight Junction Protein 1 Antibody (NBP1-85046)

Discover related pathways, diseases and genes to Tight Junction Protein 1 Antibody (NBP1-85046). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Tight Junction Protein 1 Antibody (NBP1-85046)

Discover more about diseases related to Tight Junction Protein 1 Antibody (NBP1-85046).
 

Pathways for Tight Junction Protein 1 Antibody (NBP1-85046)

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PTMs for Tight Junction Protein 1 Antibody (NBP1-85046)

Learn more about PTMs related to Tight Junction Protein 1 Antibody (NBP1-85046).

Blogs on Tight Junction Protein 1.

Zonula Occludens (ZO) the Junction Scaffolding Proteins
The Zonula Occludens (ZO) proteins 1,2 and 3, also known as tight junction proteins, are peripheral proteins localizing at junctional sites(1). ZO proteins are known to be scaffolding proteins recruiting various types of proteins to the cytoplasmic...  Read full blog post.

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Recent Reviews

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1
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Anonymous
08/26/2015
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol TJP1