Human Tie-2 ELISA Kit (Colorimetric) Summary
|Standard Curve Range
0.16 - 10.0 ng/mL
Refer to manual for specific recovery value
ELISA Kit (Colorimetric)
The human TIE-2 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human TIE-2.
Packaging, Storage & Formulations
Storage of components varies. See protocol for specific instructions.
- Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to human TIE-2
- Adhesive Films
- Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
- Human TIE-2 Standard lyophilized, 20 ng/ml upon reconstitution
- Biotin-Conjugate anti-human TIE-2 polyclonal antibody
- Sample Diluent
- Stop Solution (1M Phosphoric acid)
- Substrate Solution (tetramethyl-benzidine)
- Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Alternate Names for Human Tie-2 ELISA Kit (Colorimetric)
- angiopoietin-1 receptor
- CD202b antigen
- EC 2.7.10
- EC 220.127.116.11
- p140 TEK
- soluble TIE2 variant 1
- soluble TIE2 variant 2
- TEK tyrosine kinase, endothelial
- Tunica interna endothelial cell kinase
- Tyrosine-protein kinase receptor TEK
- Tyrosine-protein kinase receptor TIE-2
- venous malformations, multiple cutaneous and mucosal
The tyrosine kinase receptor TIE-2 was initially identified as a specific endothelial growth factor receptor that mediated several properties of endothelial cells under both physiologcal and pathological conditions. Angiopoietins are the natural ligands of TIE-2 and they induce TIE-2-dependant signalling, including survival and apoptosis of endothelial cells, vascular permeability and regulate capillary sprouting. TIE-2 plays an important role in several vascular diseases, known as vascular malformation (e.g. venous malformation). Independent of angiogenesis and its involvement in lymphangiogenesis, TIE-2 maintains a long-term, quiescent population of hematopoietc stems cells in the bone marrow. In a tumor model, a subset of monocytes was found to be positve for TIE-2 and that they have an important function in paracrine support of nascent blood vessels. A natural occuring soluble TIE-2 receptor fragment of 75 kDa (sTIE-2) is generated by shedding. Soluble TIE-2 inhibits angiopoietin-mediated TIE-2 phosphorylation and anti-apoptosis. TIE-2 shedding is mediated by PI2K/Akt and p38 MAPK. The serum levels of sTIE-2 in patients is in the lower ng/ml range and dependent of the disease. Soluble TIE-2 was also measured in preterm infants including the ROP syndrome (retinopathy of prematurity). Elevated plasma levels in active ROP patients were observed for sTIE-2. In experimental tumor mouse models sTIE-2 can be used for tumor regression and prolonged the tumor-free survival in 80% of the animals.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed
for 6 months from date of receipt.
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