Human Tie-2 ELISA Kit (Colorimetric)

Images

 
Sandwich ELISA: Human Tie-2 ELISA Kit (Colorimetric) [NBP1-83746] - Representative standard curve for human TIE-2 ELISA. Human TIE-2 was diluted in serial 2-fold steps in SampleDiluent. Do not use this standard curve to ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Standard Curve Range
0.16 - 10.0 ng/mL
Sensitivity
0.03 ng/mL

Order Details

Human Tie-2 ELISA Kit (Colorimetric) Summary

Standard Curve Range
0.16 - 10.0 ng/mL
Sensitivity
0.03 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
6.3%
Intra-Assay
7.1%
Spike Recovery
Refer to manual for specific recovery value
Sample Volume
10 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
TEK

Applications/Dilutions

Application Notes
The human TIE-2 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human TIE-2.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to human TIE-2
  2. Adhesive Films
  3. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  4. Human TIE-2 Standard lyophilized, 20 ng/ml upon reconstitution
  5. Biotin-Conjugate anti-human TIE-2 polyclonal antibody
  6. Sample Diluent
  7. Stop Solution (1M Phosphoric acid)
  8. Substrate Solution (tetramethyl-benzidine)
  9. Streptavidin-HRP
  10. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Alternate Names for Human Tie-2 ELISA Kit (Colorimetric)

  • angiopoietin-1 receptor
  • CD202b antigen
  • CD202b
  • EC 2.7.10
  • EC 2.7.10.1
  • hTIE2
  • p140 TEK
  • soluble TIE2 variant 1
  • soluble TIE2 variant 2
  • TEK tyrosine kinase, endothelial
  • TEK
  • Tie2
  • Tie-2
  • TIE2CD202b
  • Tunica interna endothelial cell kinase
  • Tyrosine-protein kinase receptor TEK
  • Tyrosine-protein kinase receptor TIE-2
  • venous malformations, multiple cutaneous and mucosal
  • VMCM
  • VMCM1

Background

The tyrosine kinase receptor TIE-2 was initially identified as a specific endothelial growth factor receptor that mediated several properties of endothelial cells under both physiologcal and pathological conditions. Angiopoietins are the natural ligands of TIE-2 and they induce TIE-2-dependant signalling, including survival and apoptosis of endothelial cells, vascular permeability and regulate capillary sprouting. TIE-2 plays an important role in several vascular diseases, known as vascular malformation (e.g. venous malformation). Independent of angiogenesis and its involvement in lymphangiogenesis, TIE-2 maintains a long-term, quiescent population of hematopoietc stems cells in the bone marrow. In a tumor model, a subset of monocytes was found to be positve for TIE-2 and that they have an important function in paracrine support of nascent blood vessels. A natural occuring soluble TIE-2 receptor fragment of 75 kDa (sTIE-2) is generated by shedding. Soluble TIE-2 inhibits angiopoietin-mediated TIE-2 phosphorylation and anti-apoptosis. TIE-2 shedding is mediated by PI2K/Akt and p38 MAPK. The serum levels of sTIE-2 in patients is in the lower ng/ml range and dependent of the disease. Soluble TIE-2 was also measured in preterm infants including the ROP syndrome (retinopathy of prematurity). Elevated plasma levels in active ROP patients were observed for sTIE-2. In experimental tumor mouse models sTIE-2 can be used for tumor regression and prolonged the tumor-free survival in 80% of the animals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol TEK
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