Human Tie-2 ELISA Kit (Colorimetric)

Sandwich ELISA: Human Tie-2 ELISA Kit (Colorimetric) [NBP1-83746] - Representative standard curve for human TIE-2 ELISA. Human TIE-2 was diluted in serial 2-fold steps in SampleDiluent. Do not use this standard curve to derive test results. A standard curve must be run for each group ofmicrowell strips assayed.

Product Discontinued

Human Tie-2 ELISA Kit (Colorimetric) Summary

• Description: The human TIE-2 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human TIE-2.
• Specificity: The assay detects both natural and recombinant human TIE-2. The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into serum. There was no crossreactivity detected, notably not with TIE-1.
• Standard Curve Range: 0.16 - 10.0 ng/mL
• Sensitivity: 0.03 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 6.3%
• Intra-Assay: CV% < 7.1%
• Spike Recovery: Refer to manual for specific recovery value
• Sample Volume: 10 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: TEK

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Tie-2 ELISA Kit (Colorimetric)

• angiopoietin-1 receptor
• CD202b antigen
• CD202b
• EC 2.7.10
• EC 2.7.10.1
• hTIE2
• p140 TEK
• soluble TIE2 variant 1
• soluble TIE2 variant 2
• TEK tyrosine kinase, endothelial
• TEK
• Tie2
• Tie-2
• TIE2CD202b
• Tunica interna endothelial cell kinase
• Tyrosine-protein kinase receptor TEK
• Tyrosine-protein kinase receptor TIE-2
• venous malformations, multiple cutaneous and mucosal
• VMCM
• VMCM1

Background

The tyrosine kinase receptor TIE-2 was initially identified as a specific endothelial growth factor receptor that mediated several properties of endothelial cells under both physiologcal and pathological conditions. Angiopoietins are the natural ligands of TIE-2 and they induce TIE-2-dependant signalling, including survival and apoptosis of endothelial cells, vascular permeability and regulate capillary sprouting. TIE-2 plays an important role in several vascular diseases, known as vascular malformation (e.g. venous malformation). Independent of angiogenesis and its involvement in lymphangiogenesis, TIE-2 maintains a long-term, quiescent population of hematopoietc stems cells in the bone marrow. In a tumor model, a subset of monocytes was found to be positve for TIE-2 and that they have an important function in paracrine support of nascent blood vessels. A natural occuring soluble TIE-2 receptor fragment of 75 kDa (sTIE-2) is generated by shedding. Soluble TIE-2 inhibits angiopoietin-mediated TIE-2 phosphorylation and anti-apoptosis. TIE-2 shedding is mediated by PI2K/Akt and p38 MAPK. The serum levels of sTIE-2 in patients is in the lower ng/ml range and dependent of the disease. Soluble TIE-2 was also measured in preterm infants including the ROP syndrome (retinopathy of prematurity). Elevated plasma levels in active ROP patients were observed for sTIE-2. In experimental tumor mouse models sTIE-2 can be used for tumor regression and prolonged the tumor-free survival in 80% of the animals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Tie-2 ELISA Kit (NBP1-83746). (Showing 1 - 1 of 1 FAQ).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: TEK