Human Tie-2 ELISA Kit (Colorimetric)

Sandwich ELISA: Human Tie-2 ELISA Kit (Colorimetric) [NBP1-83746] - Representative standard curve for human TIE-2 ELISA. Human TIE-2 was diluted in serial 2-fold steps in SampleDiluent. Do not use this standard curve to derive test results. A standard curve must be run for each group ofmicrowell strips assayed.

Product Discontinued

Human Tie-2 ELISA Kit (Colorimetric) Summary

• Description: The human TIE-2 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human TIE-2.
• Specificity: The assay detects both natural and recombinant human TIE-2. The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into serum. There was no crossreactivity detected, notably not with TIE-1.
• Standard Curve Range: 0.16 - 10.0 ng/mL
• Sensitivity: 0.03 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 6.3%
• Intra-Assay: CV% < 7.1%
• Spike Recovery: Refer to manual for specific recovery value
• Sample Volume: 10 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: TEK

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Tie-2 ELISA Kit (Colorimetric)

• angiopoietin-1 receptor
• CD202b antigen
• CD202b
• EC 2.7.10
• EC 2.7.10.1
• hTIE2
• p140 TEK
• soluble TIE2 variant 1
• soluble TIE2 variant 2
• TEK tyrosine kinase, endothelial
• TEK
• Tie2
• Tie-2
• TIE2CD202b
• Tunica interna endothelial cell kinase
• Tyrosine-protein kinase receptor TEK
• Tyrosine-protein kinase receptor TIE-2
• venous malformations, multiple cutaneous and mucosal
• VMCM
• VMCM1

Background

The tyrosine kinase receptor TIE-2 was initially identified as a specific endothelial growth factor receptor that mediated several properties of endothelial cells under both physiologcal and pathological conditions. Angiopoietins are the natural ligands of TIE-2 and they induce TIE-2-dependant signalling, including survival and apoptosis of endothelial cells, vascular permeability and regulate capillary sprouting. TIE-2 plays an important role in several vascular diseases, known as vascular malformation (e.g. venous malformation). Independent of angiogenesis and its involvement in lymphangiogenesis, TIE-2 maintains a long-term, quiescent population of hematopoietc stems cells in the bone marrow. In a tumor model, a subset of monocytes was found to be positve for TIE-2 and that they have an important function in paracrine support of nascent blood vessels. A natural occuring soluble TIE-2 receptor fragment of 75 kDa (sTIE-2) is generated by shedding. Soluble TIE-2 inhibits angiopoietin-mediated TIE-2 phosphorylation and anti-apoptosis. TIE-2 shedding is mediated by PI2K/Akt and p38 MAPK. The serum levels of sTIE-2 in patients is in the lower ng/ml range and dependent of the disease. Soluble TIE-2 was also measured in preterm infants including the ROP syndrome (retinopathy of prematurity). Elevated plasma levels in active ROP patients were observed for sTIE-2. In experimental tumor mouse models sTIE-2 can be used for tumor regression and prolonged the tumor-free survival in 80% of the animals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Tie-2 ELISA Kit (NBP1-83746). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: TEK