Tie-2 ELISA Kit


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Product Details

Concentration is not relevant for this product. Please see the protocols for proper use of this product.
Suitable Sample Type
Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)

Order Details

Tie-2 ELISA Kit Summary

Standard Curve Range
0.16 - 10.0 ng/mL
0.03 ng/mL
Kit Type

Packaging, Storage & Formulations

Storage of components varies. See protocol for specific instructions.


Application Notes
The human TIE-2 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human TIE-2.

Kit Components

  1. Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to human TIE-2
  2. Biotin-Conjugate anti-human TIE-2 polyclonal antibody
  3. Streptavidin-HRP
  4. Human TIE-2 Standard lyophilized, 20 ng/ml upon reconstitution
  5. Sample Diluent
  6. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  7. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  8. Substrate Solution (tetramethyl-benzidine)
  9. Stop Solution (1M Phosphoric acid)
  10. Adhesive Films


Colored dyes for optional use with this kit are available and will be provided free of charge upon request. Please refer to catalog number NBP2-41404.

Alternate Names for Tie-2 ELISA Kit

  • angiopoietin-1 receptor
  • CD202b antigen
  • CD202b
  • EC 2.7.10
  • EC
  • hTIE2
  • p140 TEK
  • soluble TIE2 variant 1
  • soluble TIE2 variant 2
  • TEK tyrosine kinase, endothelial
  • TEK
  • Tie2
  • TIE-2
  • TIE2CD202b
  • Tunica interna endothelial cell kinase
  • Tyrosine-protein kinase receptor TEK
  • Tyrosine-protein kinase receptor TIE-2
  • venous malformations, multiple cutaneous and mucosal
  • VMCM
  • VMCM1


The tyrosine kinase receptor TIE-2 was initially identified as a specific endothelial growth factor receptor that mediated several properties of endothelial cells under both physiologcal and pathological conditions. Angiopoietins are the natural ligands of TIE-2 and they induce TIE-2-dependant signalling, including survival and apoptosis of endothelial cells, vascular permeability and regulate capillary sprouting. TIE-2 plays an important role in several vascular diseases, known as vascular malformation (e.g. venous malformation). Independent of angiogenesis and its involvement in lymphangiogenesis, TIE-2 maintains a long-term, quiescent population of hematopoietc stems cells in the bone marrow. In a tumor model, a subset of monocytes was found to be positve for TIE-2 and that they have an important function in paracrine support of nascent blood vessels. A natural occuring soluble TIE-2 receptor fragment of 75 kDa (sTIE-2) is generated by shedding. Soluble TIE-2 inhibits angiopoietin-mediated TIE-2 phosphorylation and anti-apoptosis. TIE-2 shedding is mediated by PI2K/Akt and p38 MAPK. The serum levels of sTIE-2 in patients is in the lower ng/ml range and dependent of the disease. Soluble TIE-2 was also measured in preterm infants including the ROP syndrome (retinopathy of prematurity). Elevated plasma levels in active ROP patients were observed for sTIE-2. In experimental tumor mouse models sTIE-2 can be used for tumor regression and prolonged the tumor-free survival in 80% of the animals.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Gene Symbol TEK

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