Tie-2 Antibody (tek9) - Azide and BSA Free

Flow Cytometry: Tie-2 Antibody (tek9) [NBP1-18614] - FACS analysis with primary human dermal microvascular endothelial cells (HDMVEC).

Sandwich ELISA: Tie-2 Antibody (tek9) [NBP1-18614] - Quantification of soluble and cellular Tie-2 by sandwich ELISA. CM and cell lysates from HUVECs treated with PMA (25ng/ml) or left untreated were analysed by Sandwich ELISA for the concentrations of sTie-2 or Tie-2. For capturing anti-human Tie-2 Cl.16 NBP1-18616 was used, for the detection of a mixture of biotinylated anti-human Tie-2 Cl.2 NBP1-18612 and Cl.9 NBP1-18614.

• Reactivity: Hu
• Applications: WB, ELISA, Flow, CyTOF-ready
• Clone: tek9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Tie-2 Antibody (tek9) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Tie-2 Antibody (tek9) - Azide and BSA Free (NBP1-18614) is a monoclonal antibody validated for use in WB, ELISA and Flow. Anti-Tie-2 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human soluble extracellular TIE-2
• Specificity: The monoclonal antibody will detect native human TIE-2/tek in ELISA experiments and on the surface of different human cell types.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TEK
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1 - 2 ug/ml
  • Flow Cytometry 2 - 5 ug/ml
  • Sandwich ELISA
  • Western Blot 2 - 5 ug/ml
• Application Notes: This antibody is Cytof ready.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein G purified
• Reconstitution Instructions: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Please note the sample size of this product will be provided in reconstituted liquid form.

Alternate Names for Tie-2 Antibody (tek9) - Azide and BSA Free

• angiopoietin-1 receptor
• CD202b antigen
• CD202b
• EC 2.7.10
• EC 2.7.10.1
• hTIE2
• p140 TEK
• soluble TIE2 variant 1
• soluble TIE2 variant 2
• TEK tyrosine kinase, endothelial
• TEK
• Tie2
• Tie-2
• TIE2CD202b
• Tunica interna endothelial cell kinase
• Tyrosine-protein kinase receptor TEK
• Tyrosine-protein kinase receptor TIE-2
• venous malformations, multiple cutaneous and mucosal
• VMCM
• VMCM1

Background

TIE2 (tyrosine kinase with Ig and EGF homology domains 2) is expressed almost exclusively in endothelial cells in mice, rats and humans. This receptor possesses a unique extracellular domain containing two immunoglobulin like loops separated by three epidermal growth factor like repeats that are connected to three fibronectin type III like repeats. The ligand for the receptor is Angiopoietin 1. Defects in TIE2 are associated with inherited venous malformations; the TIE2 signaling pathway appears to be critical for endothelial cell smooth muscle cell communication in venous morphogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Tie-2 Antibody (NBP1-18614)(2)

Publications using NBP1-18614

• Zhou H, Tu Q, Zhang Y et al. Shear stress improves the endothelial progenitor cell function via the CXCR7/ERK pathway axis in the coronary artery disease cases BMC Cardiovasc Disord 2020-09-07 [PMID: 32894067] (FLOW, Human)
• Guo F, Si C, Zhou M et al. Decreased PECAM1-mediated TGF-b1 expression in the mid-secretory endometrium in women with recurrent implantation failure. Hum. Reprod. 2018-03-30 [PMID: 29617817] (Human)

Bioinformatics

• Gene Symbol: TEK