Tie-2 Antibody (MM0018-21G7) - Azide and BSA Free

Western Blot: Tie-2 Antibody (MM0018-21G7) - Azide and BSA Free [NB110-60986] - Effects of proteasome inhibitor ALLN (dose-dependent) on the vascular receptor protein expression in HUVECs.

Immunohistochemistry: Tie-2 Antibody (MM0018-21G7) - Azide and BSA Free [NB110-60986] - Formalin fixed and paraffin embedded human placental tissue section is subjected to IHC with anti human Tie-2.

Immunohistochemistry-Paraffin: Tie-2 Antibody (MM0018-21G7) [NB110-60986] - Human lymph node. Tissue section pre-treated with proteinase K for 5 minutes at 37C. Antibody at 1:50. Detection with tyramide amplification system (DAKO CSA II). IHC-P image submitted by a verified customer review.

Immunohistochemistry: Tie-2 Antibody (MM0018-21G7) - Azide and BSA Free [NB110-60986] - Formalin fixed and paraffin embedded human placental tissue section is subjected to IHC with anti human Tie-2.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: MM0018-21G7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Tie-2 Antibody (MM0018-21G7) - Azide and BSA Free Summary

• Immunogen: This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with purified human extracellular domain of Tie2 fragment).
• Specificity: Recognizes human Tie2, no cross-reactivity with human Tie1.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TEK
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:200 - 1:400
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:500 - 1:1000
• Control:

  Tie-2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein G purified
• Reconstitution Instructions: Reconstitute with sterilized PBS to a final concentration of 0.2 mg/ml.

Alternate Names for Tie-2 Antibody (MM0018-21G7) - Azide and BSA Free

• angiopoietin-1 receptor
• CD202b antigen
• CD202b
• EC 2.7.10
• EC 2.7.10.1
• hTIE2
• p140 TEK
• soluble TIE2 variant 1
• soluble TIE2 variant 2
• TEK tyrosine kinase, endothelial
• TEK
• Tie2
• Tie-2
• TIE2CD202b
• Tunica interna endothelial cell kinase
• Tyrosine-protein kinase receptor TEK
• Tyrosine-protein kinase receptor TIE-2
• venous malformations, multiple cutaneous and mucosal
• VMCM
• VMCM1

Background

TIE2 (tyrosine kinase with Ig and EGF homology domains 2) is expressed almost exclusively in endothelial cells in mice, rats and humans. This receptor possesses a unique extracellular domain containing two immunoglobulin-like loops separated by three epidermal growth factor-like repeats that are connected to three fibronectin type III-like repeats. The ligand for the receptor is Angiopoietin 1. Defects in TIE2 are associated with inherited venous malformations; the TIE2 signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Tie-2 Antibody (NB110-60986)(1)

Publication using NB110-60986

• Rodrigues-Pinto R, Berry A, Piper-Hanley K et al. Spatiotemporal analysis of putative notochordal cell markers reveals CD24 and keratins 8, 18, and 19 as notochord-specific markers during early human intervertebral disc development. J Orthop Res 2016-08-01 [PMID: 26910849] (IF/IHC)

Review for Tie-2 Antibody (NB110-60986) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Masahiro Hirata IHC-P Human 03/02/2020

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Lymph node
• Species: Human
• Lot: 2019120901

Comments

• Comments: pretreatment with proteinase K, 5min at 37C.
  Tie-2 Ab was used in 4ug/mL (1:50) and detected with tyramide amplification system (DAKO CSA II).
  heating epitope retrieval was not suitable for FFPE section.

FAQs for Tie-2 Antibody (NB110-60986). (Showing 1 - 3 of 3 FAQ).

1. Do you have any suggestions for pretreatment or dilutions with the Tie-2 (MM0018-21G7) antibody for IHC staining of FFPE tissues?
   • We recommend a pretreatment of: 1. Deparaffinize sections in xylene, 2x5min. 2. Hydrate with 100% ethanol, 2x3min. 3. Hydrate with 95% ethanol, 1min. 4. Rinse in distilled water. We also recommend a starting dilution of 1:50, and optimizing the dilution depending on how strongly expressed the protein is in your particular samples. Antigen retrieval may be necessary to unmask the epitope, and our lab finds heat mediated citrate buffer at a pH of 6.0 to be useful for this step.
2. Is antigen retrieval needed for FFPE tissues?
   • Yes, we usually recommend heat induced antigen retrieval for paraffin-embedded tissue samples, this product included. Please follow the following link for our recommended antigen retrieval protocol.
3. I've recently bought your Tie2 antibody (NB110-60986) and want to use it for IHC on FFPE sections. Can you please tell me which antigen retrieval and what dilution was used there?
   • For antigen retrieval, the sections were treated with 20 ug/ml proteinase K in PBS for 15 minutes at room temperature. The antibody dilution was 1:50.

Bioinformatics

• Gene Symbol: TEK
• Entrez:
  • 7010(Human)
• Uniprot:
  • Q02763()