Western Blot: Thymidine Kinase 1 Antibody [NBP2-20626] - Wild-type (WT) and Thymidine Kinase 1 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Thymidine Kinase ...read more
Immunohistochemistry-Paraffin: Thymidine Kinase 1 Antibody [NBP2-20626] - Thymidine Kinase 1 antibody detects Thymidine Kinase 1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat ovary. ...read more
Western Blot: Thymidine Kinase 1 Antibody [NBP2-20626] - A431 whole cell and cytoplasm extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Thymidine Kinase 1 antibody diluted at 1:1000.
Western Blot: Thymidine Kinase 1 Antibody [NBP2-20626] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Thymidine Kinase 1 antibody diluted at 1:1000. ...read more
Immunohistochemistry-Paraffin: Thymidine Kinase 1 Antibody [NBP2-20626] - Human colon carcinoma, using Thymidine Kinase 1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, ...read more
25 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publication using NBP2-20626 in the following applications:
Chicken (83%), Zebrafish (81%). Rat reactivity reported in scientific literature (PMID: 26087030).
Packaging, Storage & Formulations
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS, 20% Glycerol (pH7).
0.025% Proclin 300
Immunogen affinity purified
Alternate Names for Thymidine Kinase 1 Antibody
thymidine kinase 1 soluble isoform
Thymidine Kinase 1
thymidine kinase 1, soluble
thymidine kinase, cytosolic
Thymidine kinase (TK) belongs to a group of enzymes such as dihydrofolate reductase, thymidylate synthase, and DNA polymerase that are involved in DNA synthesis and precursor production. Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells due to multiple regulatory mechanisms that ensure expression of these enzymes exclusively in replicating cells. TK is responsible for catalyzing the phosphorylation of thymidine, which functions as a part of the pyrimidine salvage pathway involved in DNA synthesis. The activities of enzymes such as TK are essential for the activation of several chemotherapeutically important nucleoside analogues that are administered as prodrugs.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.
Publications for Thymidine Kinase 1 Antibody (NBP2-20626)(1)
We have publications tested in 1 confirmed species: Rat.
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