Thymidine Kinase 1 Antibody - BSA Free

Western Blot: Thymidine Kinase 1 Antibody [NBP2-20626] - Wild-type (WT) and Thymidine Kinase 1 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Thymidine Kinase 1 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody

Immunohistochemistry-Paraffin: Thymidine Kinase 1 Antibody [NBP2-20626] - Thymidine Kinase 1 antibody detects Thymidine Kinase 1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat ovary. Thymidine Kinase 1 stained by Thymidine Kinase 1 antibody diluted at 1:500.

Western Blot: Thymidine Kinase 1 Antibody [NBP2-20626] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Thymidine Kinase 1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: Thymidine Kinase 1 Antibody [NBP2-20626] - Human colon carcinoma, using Thymidine Kinase 1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: Thymidine Kinase 1 Antibody [NBP2-20626] - Mouse muscle, using Thymidine Kinase 1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

A431 whole cell and cytoplasm extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Thymidine Kinase 1 antibody (NBP2-20626) diluted at 1:1000.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, IHC, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Thymidine Kinase 1 Antibody - BSA Free Summary

• Description: Novus Biologicals Knockout (KO) Validated Rabbit Thymidine Kinase 1 Antibody - BSA Free (NBP2-20626) is a polyclonal antibody validated for use in IHC and WB. Anti-Thymidine Kinase 1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Thymidine Kinase 1. The exact sequence is proprietary.
• Localization: Cytoplasm
• Predicted Species: Bovine (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TK1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockout Validated
  • Western Blot 1:500-1:3000
• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken (83%), Zebrafish (81%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Thymidine Kinase 1 Antibody - BSA Free

• EC 2.7.1.21
• thymidine kinase 1 soluble isoform
• Thymidine Kinase 1
• thymidine kinase 1, soluble
• thymidine kinase, cytosolic
• thymidine kinase-1
• TK1
• TK2

Background

Thymidine kinase (TK) belongs to a group of enzymes such as dihydrofolate reductase, thymidylate synthase, and DNA polymerase that are involved in DNA synthesis and precursor production. Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells due to multiple regulatory mechanisms that ensure expression of these enzymes exclusively in replicating cells. TK is responsible for catalyzing the phosphorylation of thymidine, which functions as a part of the pyrimidine salvage pathway involved in DNA synthesis. The activities of enzymes such as TK are essential for the activation of several chemotherapeutically important nucleoside analogues that are administered as prodrugs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Thymidine Kinase 1 Antibody (NBP2-20626)(1)

Publication using NBP2-20626

• Agarwal HK, Khalil A, Ishita K et al. Synthesis and evaluation of thymidine kinase 1-targeting carboranyl pyrimidine nucleoside analogs for boron neutron capture therapy of cancer Eur J Med Chem. 2015-05-27 [PMID: 26087030] (WB, Rat)

Bioinformatics

• Gene Symbol: TK1
• Uniprot:
  • P04183()