EPCR Antibody [Unconjugated]

Images

 
Western blot shows lysates of human skin tissue, human placenta tissue, and HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human EPCR Antigen ...read more
EPCR was detected in immersion fixed paraffin-embedded sections of human liver using 1.7 µg/mL Goat Anti-Human EPCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2245) overnight at 4 °C. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

EPCR Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human EPCR
Ser18-Ser210
Accession # Q9UNN8
Specificity
Detects human EPCR in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant mouse EPCR is observed.
Details of Functionality
ActivityAssaywCitation not found None None None
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PROCR
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.25 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 1-15 ug/mL
  • CyTOF-ready
Publications
Read Publications using
AF2245 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EPCR Antibody [Unconjugated]

  • APC receptor
  • CCCA
  • CCD41
  • CCD41centrosome-associated protein
  • CD201 antigen
  • CD201
  • centrocyclin
  • Endothelial cell protein C receptor
  • endothelial protein C receptor
  • EPCR
  • EPCRMGC23024
  • PROCR
  • protein C receptor, endothelial

Background

EPCR is a type I transmembrane glycoprotein in the CD1/MHC family. It is expressed most strongly in the endothelial cells of arteries and veins in heart and lung. Membrane bound EPCR is released by metalloproteolytic cleavage to generate the soluble receptor. Protein C is a vitamin K-dependent serine protease that plays a major role in blood coagulation. Binding of Protein C to EPCR leads to the proteolytic activation of PAR1 (protease-activated receptor 1) on endothelial cells and subsequent up-regulation of Protein C-induced genes.  The extracellular domain of human and mouse EPCR shares approximately 61% amino acid sequence homology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EPCR Antibody (AF2245)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 6 applications: Binding Assay, Dot Blot, ELISA Development, Flow Cytometry, ICC, IHC-P.


Filter By Application
Binding Assay
(1)
Dot Blot
(1)
ELISA Development
(1)
Flow Cytometry
(3)
ICC
(1)
IHC-P
(1)
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Filter By Species
Human
(8)
All Species
Showing Publications 1 - 8 of 8.
Publications using AF2245 Applications Species
M Avril, M Benjamin, MM Dols, JD Smith Interplay of Plasmodium falciparum and thrombin in brain endothelial barrier disruption Sci Rep, 2019;9(1):13142. 2019 [PMID: 31511575] (Flow Cytometry, Human) Flow Cytometry Human
G Ecklu-Mens, RW Olsen, A Bengtsson, MF Ofori, L Hviid, ATR Jensen, Y Adams Blood outgrowth endothelial cells (BOECs) as a novel tool for studying adhesion of Plasmodium falciparum-infected erythrocytes PLoS ONE, 2018;13(10):e0204177. 2018 [PMID: 30300360] (Flow Cytometry, Human) Flow Cytometry Human
Y Azasi, G Lindergard, A Ghumra, J Mu, LH Miller, JA Rowe Infected erythrocytes expressing DC13 PfEMP1 differ from recombinant proteins in EPCR-binding function Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):. 2018 [PMID: 29339517] (Binding Assay, Human) Binding Assay Human
Interaction between Endothelial Protein C Receptor and Intercellular Adhesion Molecule 1 to Mediate Binding of Plasmodium falciparum-Infected Erythrocytes to Endothelial Cells MBio, 2016;7(4):. 2016 [PMID: 27406562] (Flow Cytometry, Human) Flow Cytometry Human
Campbell JE, Brummel-Ziedins KE, Butenas S Cellular regulation of blood coagulation: a model for venous stasis. Blood, 2010;116(26):6082-91. 2010 [PMID: 20864579] (ICC, Human) ICC Human
Gunawardana CG, Kuk C, Smith CR Comprehensive analysis of conditioned media from ovarian cancer cell lines identifies novel candidate markers of epithelial ovarian cancer. J. Proteome Res., 2009;8(10):4705-13. 2009 [PMID: 19663500] (ELISA Development, Human) ELISA Development Human
Ducros E, Berthaut A, Mirshahi SS, Faussat AM, Soria J, Agarwal MK, Mirshahi M Aldosterone modifies hemostasis via upregulation of the protein-C receptor in human vascular endothelium. Biochem. Biophys. Res. Commun., 2008;373(2):192-6. 2008 [PMID: 18555797] (Dot Blot, Human) Dot Blot Human
Xue M, Campbell D, Jackson CJ Protein C is an autocrine growth factor for human skin keratinocytes. J. Biol. Chem., 2007;282(18):13610-6. 2007 [PMID: 17293597] (IHC-P, Human) IHC-P Human

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Secondary Antibodies

 

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Other Available Formats

Biotin BAF2245

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Bioinformatics

Gene Symbol PROCR
Uniprot