Genetic Strategies: Knockout Validated: TGN46 Antibody [NBP1-49643] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and TGN46 knockout (KO) HeLa cell line. PVDF ...read more
Western Blot: TGN46 Antibody [NBP1-49643] - Western blot analysis of extracts from HeLa cells using TGN46 antibody (NBP1-49643, 1:100).
Immunocytochemistry/ Immunofluorescence: TGN46 Antibody [NBP1-49643] - T98G glioblastoma cells probed for Golgi (Alexa Fluor 488 conjugated TGN-46 antibody, Green), tubulin (Alexa Fluor 594, Red), and nucleus (DAPI, ...read more
Immunohistochemistry: TGN46 Antibody [NBP1-49643] - Immunohistochemical analysis of TGOLN2 in human renal cell carcinoma.
Flow (Intracellular): TGN46 Antibody [NBP1-49643] - An intracellular stain was performed on HepG2 cells with TGN46 Antibody NBP1-49643AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and ...read more
Immunocytochemistry/ Immunofluorescence: TGN46 Antibody [NBP1-49643] - IF Confocal analysis of A549 cells using TGN46 antibody (NBP1-49643, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary ...read more
Immunocytochemistry/ Immunofluorescence: TGN46 Antibody [NBP1-49643] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were ...read more
Immunocytochemistry/ Immunofluorescence: TGN46 Antibody [NBP1-49643] - Antibody was tested at 1:100 in HeLa cells with FITC (green).
Immunocytochemistry/ Immunofluorescence: TGN46 Antibody [NBP1-49643] - TGOLN2 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
Flow (Intracellular): TGN46 Antibody [NBP1-49643] - An intracellular stain was performed on U-937 cells with TGN46 Antibody NBP1-49643 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more
This TGOLN2 antibody is useful for Immunohistochemistry on paraffin tissues and Immunocytochemistry/Immunofluorescence. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Read 2 Reviews rated 5 using NBP1-49643 in the following applications:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS and 30% Glycerol
0.05% Sodium Azide
Immunogen affinity purified
Manufactured by Genomic Antibody Technology™. GAT FAQs
Alternate Names for TGN46 Antibody
trans golgi network 38
trans golgi network 46
Trans Golgi network integral membrane protein 2 [Precursor]
Trans Golgi network integral membrane protein 2
Trans Golgi network protein (46 48 51kD isoforms)
Trans golgi network protein 2
Trans Golgi network protein TGN51
Trans-Golgi network integral membrane protein 2
Trans-Golgi network protein 2
Trans-Golgi network protein TGN51
TGN46 (TGOLN2/trans-Golgi network protein 2) is a single-pass type I membrane protein localized in cell membrane as well as in trans-Golgi network (TGN) membrane, an important protein sorting station inside cells. It shuttles between TGN and cell surface returning via endosomes. TGN46-vesicle formation requires protein kinase D, a kinase needed for secretory carrier formation in cells. TGOLN2 gene has several alternatively spliced transcripts, of which isoform TGN46 is widely expressed whereas another isoform TGN51 is more abundant in fetal lung and kidney. TGN46 is a heavily glycosylated protein with largely unknown functions, however, it has been suspected to be involved in regulating membrane traffic to and from the TGN. TGN46 contains a signal peptide, lumenal domain, membrane-spanning domain, and cytoplasmic domain. Because the membrane spanning region and cytoplasmic tail contain the retention and retrieval signals, respectively, for localization in the TGN, TGN46 is considered a molecular marker for TGN. Recent expression studies have shown a connection between TGOLN2 and suicidal behavior wherein TGN46 was found to be up-regulated in suicide completers as compared to controls.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
IF Confocal analysis of A549 cells using TGN46 antibody (NBP1-49643, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
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