TGN46 Antibody - BSA Free

Knockout Validated: TGN46 Antibody [NBP1-49643] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and TGN46 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human TGN46 Polyclonal Antibody (NBP1-49643) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). Specific band was detected for TGN46 at approximately 100 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.

Immunocytochemistry/Immunofluorescence: TGN46 Antibody [NBP1-49643] - A7 cells expressing F-NTPase were fixed and dual stained with an anti-FLAG antibody (green) and TGN46 antibody (red). Colocalization of F-NTPase with the signals of organelle-specific markers are shown in yellow (arrows). The dashed boxes in each merged image were enlarged and are shown at the right. As noted, the nonvesicular areas of F-NTPase does not colocalize with the signals of TGN46 (a trans-Golgi marker). Scale bars, 10 um. Image collected and cropped by CiteAb from the following publication (//jvi.asm.org/content/92/5/e01824-17) licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: TGN46 Antibody [NBP1-49643] - U-2 OS cells were transfected with siRNAs targeting UGCG (siUGCG) or a non-targeting control (siNegCtrl) and plated onto glass coverslips. At 72 hours post-transfection cells were chilled to 4 degrees C on ice and rVSV-SFTSV was bound by centrifugation (1200xg, 30', 4 degrees C). Following centrifugation, media was replaced with pre-warmed media (37 degrees C) and the cells placed in a 37 degrees C incubator for 40 minutes before fixation in 1% paraformaldehyde for 15 minutes. Cells were then immunostained for viral antigen (anti-VSV M, red), TGN46 (green), and nuclei stained with DAPI (blue). Images are representative from at least 3 independent experiments. Boxes indicate zoomed-in regions. Scale bar represents 5um. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1006316) licensed under a CC-BY licence.

Immunohistochemistry: TGN46 Antibody [NBP1-49643] - Analysis of TGOLN2 in human renal cell carcinoma.

Immunocytochemistry/Immunofluorescence: TGN46 Antibody [NBP1-49643] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with TGN46 Antibody conjugated to Alexa Fluor 488 (NBP1-49643AF488) at 2 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Flow Cytometry: TGN46 Antibody [NBP1-49643] - An intracellular stain was performed on HepG2 cells with NBP1-49643C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.

Western Blot: TGN46 Antibody [NBP1-49643] - Analysis of extracts from HeLa cells using TGN46 antibody (NBP1-49643, 1:100).

Immunocytochemistry/Immunofluorescence: TGN46 Antibody [NBP1-49643] - HepG2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with TGN46 Antibody conjugated to Alexa Fluor 488 (NBP1-49643AF488) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunocytochemistry/Immunofluorescence: TGN46 Antibody [NBP1-49643] - Analysis of A549 cells using TGN46 antibody (NBP1-49643, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).

Immunocytochemistry/Immunofluorescence: TGN46 Antibody [NBP1-49643] - T98G glioblastoma cells probed for Golgi (Alexa Fluor 488 conjugated TGN-46 antibody, Green), tubulin (Alexa Fluor 594, Red), and nucleus (DAPI, blue). Image from the Alexa Fluor 488 version of this antibody. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: TGN46 Antibody [NBP1-49643] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- at 2 ug/ml for 60 minutes at room temperature and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective.

Flow (Intracellular): TGN46 Antibody [NBP1-49643] - An intracellular stain was performed on U-937 cells with NBP1-49643 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).

Flow (Intracellular): TGN46 Antibody [NBP1-49643] - An intracellular stain was performed on HepG2 cells with TGN46 Antibody NBP1-49643AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

• Reactivity: Hu, Mu, Bv, Pm
• Applications: WB, Flow, Flow-IC, IB, ICC/IF, IHC, IHC-P, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

TGN46 Antibody - BSA Free Summary

• Immunogen: A genomic peptide made to an internal region of the human TGN46 protein (within residues 200-350). [Swiss-Prot: O43493]
• Localization: Primarily in trans-Golgi network. Cycles between the trans-Golgi network and the cell surface returning via endosomes
• Marker: TGN Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TGOLN2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
  • Knockout Validated
  • Western Blot 2ug/mL
• Application Notes: Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in immunoblotting reported in scientific literature (PMID: 25410859). Use in ICC/IF was reported in scientific literature (PMID: 31455601).

Reactivity Notes

Monkey reactivity reported in scientific literature (PMID: 30135710). Mouse reactivity reported in scientific literature (PMID: 26980706). Bovine reactivity reported in scientific literature (PMID: 30135710).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for TGN46 Antibody - BSA Free

• TGN 46
• TGN 48
• TGN 51
• TGN38 homolog
• TGN38
• TGN-38
• TGN46
• TGN-46
• TGN48
• TGN51
• TGOLN 2
• TGOLN2
• trans golgi network 38
• trans golgi network 46
• Trans Golgi network integral membrane protein 2 [Precursor]
• Trans Golgi network integral membrane protein 2
• Trans Golgi network protein (46 48 51kD isoforms)
• Trans golgi network protein 2
• Trans Golgi network protein TGN51
• Trans-Golgi network integral membrane protein 2
• Trans-Golgi network protein 2
• Trans-Golgi network protein TGN51
• TTGN 2
• TTGN2

Background

TGN46 (TGOLN2/trans-Golgi network protein 2) is a single-pass type I membrane protein localized in cell membrane as well as in trans-Golgi network (TGN) membrane, an important protein sorting station inside cells. It shuttles between TGN and cell surface returning via endosomes. TGN46-vesicle formation requires protein kinase D, a kinase needed for secretory carrier formation in cells. TGOLN2 gene has several alternatively spliced transcripts, of which isoform TGN46 is widely expressed whereas another isoform TGN51 is more abundant in fetal lung and kidney. TGN46 is a heavily glycosylated protein with largely unknown functions, however, it has been suspected to be involved in regulating membrane traffic to and from the TGN. TGN46 contains a signal peptide, lumenal domain, membrane-spanning domain, and cytoplasmic domain. Because the membrane spanning region and cytoplasmic tail contain the retention and retrieval signals, respectively, for localization in the TGN, TGN46 is considered a molecular marker for TGN. Recent expression studies have shown a connection between TGOLN2 and suicidal behavior wherein TGN46 was found to be up-regulated in suicide completers as compared to controls.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TGN46 Antibody (NBP1-49643)(23)

Publications using NBP1-49643

• Steimle BL, Bailey DK, Smith FM Et al. Calcium and the Ca-ATPase SPCA1 modulate plasma membrane abundance of ZIP8 and ZIP14 to regulate Mn(II) uptake in brain microvascular endothelial cells J Biol Chem 2022-07-05 [PMID: 35787370] (ICC/IF, Human)
  
  Details:
  Citation using the Alexa Fluor 647 version of this antibody.
• Song P, Peng J, Omrane M et al. Septin 9 and phosphoinositides regulate lysosome localization and their association with lipid droplets iScience [PMID: 35573204]
• Okada T, Nishida S, Zhang L Et al. Constitutive activation of S1P receptors at the trans-Golgi network is required for surface transport carrier formation iScience 2021-11-19 [PMID: 34805799] (ICC/IF, Human)
• Kutchukian C, Vivas O, Casas M et al. NPC1 regulates the distribution of phosphatidylinositol 4-kinases at Golgi and lysosomal membranes The EMBO journal 2021-05-21 [PMID: 34019311]
• Bakkar N, Starr A, Rabichow BE et al. The M1311V variant of ATP7A is associated with impaired trafficking and copper homeostasis in models of motor neuron disease Neurobiology of disease 2020-12-20 [PMID: 33359139] (ICC/IF)
• Roy S, McCann CJ, Ralle M et al. Analysis of Wilson disease mutations revealed that interactions between different ATP7B mutants modify their properties Sci Rep 2020-08-10 [PMID: 32778786] (ICC/IF, Human)
• McPhail JA, Lyoo H, Pemberton JG, Hoffmann RM Characterisation of the c10orf76-PI4KB complex, and its necessity for Golgi PI4P levels and enterovirus replication EMBO Rep 2019-12-13 [PMID: 31829496]
• Dudek A M, Zabaleta N et al. GPR108 Is a Highly Conserved AAV Entry Factor. Mol Ther 2019-11-13 [PMID: 31784416] (ICC/IF, Human)
• Del Olmo T, Lacarriere-Keita C, Normandin C et al. RAB21 interacts with TMED10 and modulates its localization and abundance Biol Open 2019-08-27 [PMID: 31455601] (ICC/IF, Human)
• Del Olmo T, Lauzier A, Normandin C et al. APEX2-mediated RAB proximity labeling identifies arole for RAB21 in clathrin-independent cargosorting EMBO Rep. 2019-01-03 [PMID: 30610016] (ICC/IF, Human)
• Roberts BL, Severance ZC, Bensen RC et al. Persistent, Multi-Generational Reduction of Oxysterol-Binding Protein Caused by Compound Treatment Induces Prophylactic Anti-Viral Activity. ACS Chem. Biol. 2018-12-21 [PMID: 30576108] (ICC/IF, Human)
• Capone V, Clemente E, Restelli E et al. PERK inhibition attenuates the abnormalities of the secretory pathway and the increased apoptotic rate induced by SIL1 knockdown in HeLa cells. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease 2018-07-01 [PMID: 30293566] (ICC/IF, Human)
• Wild P, Kaech A, Schraner EM et al. Endoplasmic reticulum-to-Golgi transitions upon herpes virus infection F1000Res. [PMID: 30135710] (ICC/IF, Bovine, Monkey)
• Melia CE, van der Schaar HM, de Jong AWM et al. The Origin, Dynamic Morphology, and PI4P-Independent Formation of Encephalomyocarditis Virus Replication Organelles MBio 2018-04-17 [PMID: 29666283] (ICC/IF, Human)
• Badawy SMM, Okada T, Kajimoto T et al. DHHC5-mediated palmitoylation of S1P receptor subtype 1 determines G-protein coupling Sci Rep 2017-11-29 [PMID: 29185452] (ICC/IF, Human)
• Becher A, Eiseler T, Porzner M et al. The armadillo protein p0071 controls KIF3 motor transport J. Cell. Sci. 2017-08-14 [PMID: 28808088] (Human)
• Serfass JM, Takahashi Y, Zhou Z et al. Endophilin B2 facilitates endosome maturation in response to growth factor stimulation, autophagy induction, and influenza A virus infection J. Biol. Chem. 2017-06-16 [PMID: 28455444] (WB)
• Drake M. J, Brennan B, et al. A role for glycolipid biosynthesis in severe fever with thrombocytopenia syndrome virus entry. PLoS Pathog 2017-04-01 [PMID: 28388693] (ICC/IF, Human)
• Takahashi Y, Tsotakos N, Liu Y et al. The Bif-1-Dynamin 2 membrane fission machinery regulates Atg9-containing vesicle generation at the Rab11-positive reservoirs. Oncotarget. 2016-04-12 [PMID: 26980706]
• Pillay S, Meyer NL, Puschnik AS et al. An essential receptor for adeno-associated virus infection Nature 2016-02-04 [PMID: 26814968] (ICC/IF, Human)
• Nonnenmacher Me, Cintrat Jc, Gillet D, Weber T. Syntaxin 5-Dependent Retrograde Transport to the Golgi is Required for AAV Transduction. J. Virol. 2014-11-19 [PMID: 25410859]
• Zhao Y, Wang Y, Hu J, Zhang X, Zhang YW. CutA divalent cation tolerance homolog (E. coli) (CUTA) regulates Beta-cleavage of Beta-amyloid precursor protein (APP) through interacting with Beta-Site APP cleaving protein 1 (BACE1) J Biol Chem 2012-02-17 [PMID: 22351782] (WB, Human)
• Donsante A, Yi L, Zerfas PM, Brinster LR, Sullivan P, Goldstein DS, Prohaska J, Centeno JA, Rushing E, Kaler SG. ATP7A gene addition to the choroid plexus results in long-term rescue of the lethal copper transport defect in a Menkes disease mouse model. Mol Ther;19(12):2114-23. 2011-12-01 [PMID: 21878905] (ICC/IF, Human)

Reviews for TGN46 Antibody (NBP1-49643) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Verified Customer ICC Human 06/12/2014

Summary

• Application: Immunocytochemistry
• Species: Human

reviewed by: Verified Customer WB Human 06/12/2014

Summary

• Application: Western Blot
• Species: Human

Bioinformatics

• Gene Symbol: TGOLN2
• Entrez:
  • 10618(Human)
• Uniprot:
  • O43493(Human)