TGN46 Antibody

Images

 
Genetic Strategies: Knockout Validated: TGN46 Antibody [NBP1-49643] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and TGN46 knockout (KO) HeLa cell line. PVDF ...read more
Western Blot: TGN46 Antibody [NBP1-49643] - Analysis of extracts from HeLa cells using TGN46 antibody (NBP1-49643, 1:100).
Immunocytochemistry/ Immunofluorescence: TGN46 Antibody [NBP1-49643] - T98G glioblastoma cells probed for Golgi (Alexa Fluor 488 conjugated TGN-46 antibody, Green), tubulin (Alexa Fluor 594, Red), and nucleus (DAPI, ...read more
Immunohistochemistry: TGN46 Antibody [NBP1-49643] - Analysis of TGOLN2 in human renal cell carcinoma.
Flow Cytometry: TGN46 Antibody [NBP1-49643] - An intracellular stain was performed on HepG2 cells with NBP1-49643C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with ...read more
Immunocytochemistry/ Immunofluorescence: TGN46 Antibody [NBP1-49643] - Analysis of A549 cells using TGN46 antibody (NBP1-49643, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody ...read more
Immunocytochemistry/ Immunofluorescence: TGN46 Antibody [NBP1-49643] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were ...read more
Immunocytochemistry/ Immunofluorescence: TGN46 Antibody [NBP1-49643] - Antibody was tested at 1:100 in HeLa cells with FITC (green).
Immunocytochemistry/ Immunofluorescence: TGN46 Antibody [NBP1-49643] - TGOLN2 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 ...read more
Flow (Intracellular): TGN46 Antibody [NBP1-49643] - An intracellular stain was performed on U-937 cells with NBP1-49643 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized ...read more
Flow (Intracellular): TGN46 Antibody [NBP1-49643] - An intracellular stain was performed on HepG2 cells with TGN46 Antibody NBP1-49643AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and ...read more

Product Details

Summary
Reactivity Hu, Mu, MkSpecies Glossary
Applications WB, Flow, Flow-IC, IB, ICC/IF, IHC, IHC-P, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.66 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

TGN46 Antibody Summary

Immunogen
A genomic peptide made to an internal region of the human TGN46 protein (within residues 200-350). [Swiss-Prot: O43493]
Localization
Primarily in trans-Golgi network. Cycles between the trans-Golgi network and the cell surface returning via endosomes
Marker
TGN Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TGOLN2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
  • Flow (Intracellular)
  • Knockout Validated
Application Notes
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in immunoblotting reported in scientific literature (PMID: 25410859).
Reviewed Applications
Read 2 Reviews rated 5
using
NBP1-49643 in the following applications:

Publications
Read Publications using
NBP1-49643 in the following applications:

  • 10 publications
  • WB
    2 publications

Reactivity Notes

Monkey reactivity reported in scientific literature (PMID: 30135710). Mouse reactivity reported in scientific literature (PMID: 26980706).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
0.66 mg/ml
Purity
Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for TGN46 Antibody

  • TGN 46
  • TGN 48
  • TGN 51
  • TGN38 homolog
  • TGN38
  • TGN-38
  • TGN46
  • TGN-46
  • TGN48
  • TGN51
  • TGOLN 2
  • TGOLN2
  • trans golgi network 38
  • trans golgi network 46
  • Trans Golgi network integral membrane protein 2 [Precursor]
  • Trans Golgi network integral membrane protein 2
  • Trans Golgi network protein (46 48 51kD isoforms)
  • Trans golgi network protein 2
  • Trans Golgi network protein TGN51
  • Trans-Golgi network integral membrane protein 2
  • Trans-Golgi network protein 2
  • Trans-Golgi network protein TGN51
  • TTGN 2
  • TTGN2

Background

TGN46 (TGOLN2/trans-Golgi network protein 2) is a single-pass type I membrane protein localized in cell membrane as well as in trans-Golgi network (TGN) membrane, an important protein sorting station inside cells. It shuttles between TGN and cell surface returning via endosomes. TGN46-vesicle formation requires protein kinase D, a kinase needed for secretory carrier formation in cells. TGOLN2 gene has several alternatively spliced transcripts, of which isoform TGN46 is widely expressed whereas another isoform TGN51 is more abundant in fetal lung and kidney. TGN46 is a heavily glycosylated protein with largely unknown functions, however, it has been suspected to be involved in regulating membrane traffic to and from the TGN. TGN46 contains a signal peptide, lumenal domain, membrane-spanning domain, and cytoplasmic domain. Because the membrane spanning region and cytoplasmic tail contain the retention and retrieval signals, respectively, for localization in the TGN, TGN46 is considered a molecular marker for TGN. Recent expression studies have shown a connection between TGOLN2 and suicidal behavior wherein TGN46 was found to be up-regulated in suicide completers as compared to controls.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-49643
Species: Hu, Mu, Mk
Applications: WB, Flow, Flow-IC, IB, ICC/IF, IHC, IHC-P, KO

Publications for TGN46 Antibody (NBP1-49643)(15)

We have publications tested in 3 confirmed species: Human, Bovine, Monkey.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(10)
WB
(2)
All Applications
Filter By Species
Human
(10)
Bovine
(2)
Monkey
(2)
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Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using NBP1-49643 Applications Species
Capone V, Clemente E, Restelli E et al. PERK inhibition attenuates the abnormalities of the secretory pathway and the increased apoptotic rate induced by SIL1 knockdown in HeLa cells. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease Jul 1 2018 [PMID: 30293566] (ICC/IF, Human) ICC/IF Human
McPhail JA, Lyoo H, Pemberton JG, Hoffmann RM Characterisation of the c10orf76-PI4KB complex, and its necessity for Golgi PI4P levels and enterovirus replication bioRxiv May 10 2019 (ICC/IF, Human) ICC/IF Human
Del Olmo T, Lauzier A, Normandin C et al. APEX2-mediated RAB proximity labeling identifies arole for RAB21 in clathrin-independent cargosorting EMBO Rep. Jan 3 2019 [PMID: 30610016] (ICC/IF, Human) ICC/IF Human
Roberts BL, Severance ZC, Bensen RC et al. Persistent, Multi-Generational Reduction of Oxysterol-Binding Protein Caused by Compound Treatment Induces Prophylactic Anti-Viral Activity. ACS Chem. Biol. Dec 21 2018 [PMID: 30576108] (ICC/IF, Human) ICC/IF Human
Wild P, Kaech A, Schraner EM et al. Endoplasmic reticulum-to-Golgi transitions upon herpes virus infection f1000research.com 2018 Feb 28 [PMID: 30135710] (ICC/IF, Monkey, Bovine) ICC/IF Monkey, Bovine
Wild P, Kaech A, Schraner EM et al. Endoplasmic reticulum-to-Golgi transitions upon herpes virus infection F1000Res 2018 Feb 28 [PMID: 30135710] (ICC/IF, Monkey, Bovine) ICC/IF Monkey, Bovine
Melia CE, van der Schaar HM, de Jong AWM et al. The Origin, Dynamic Morphology, and PI4P-Independent Formation of Encephalomyocarditis Virus Replication Organelles MBio 2018 Apr 17 [PMID: 29666283] (ICC/IF, Human) ICC/IF Human
Badawy SMM, Okada T, Kajimoto T et al. DHHC5-mediated palmitoylation of S1P receptor subtype 1 determines G-protein coupling Sci Rep 2017 Nov 29 [PMID: 29185452] (ICC/IF, Human) ICC/IF Human
Becher A, Eiseler T, Porzner M et al. The armadillo protein p0071 controls KIF3 motor transport J. Cell. Sci. 2017 Aug 14 [PMID: 28808088] (Human) Human
Serfass JM, Takahashi Y, Zhou Z et al. Endophilin B2 facilitates endosome maturation in response to growth factor stimulation, autophagy induction, and influenza A virus infection J. Biol. Chem. Jun 16 2017 [PMID: 28455444] (WB) WB
Show All 15 Publications.

Reviews for TGN46 Antibody (NBP1-49643) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NBP1-49643:
Filter by Applications
ICC
(1)
WB
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry TGN46 NBP1-49643
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reviewed by:
Anony- mous
ICC Human 06/12/2014
View

Summary

ApplicationImmunocytochemistry
SpeciesHuman
Special ApplicationsIF Confocal analysis of A549 cells using TGN46 antibody (NBP1-49643, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
FileView PDF
Western Blot TGN46 NBP1-49643
Enlarge
reviewed by:
Anony- mous
WB Human 06/12/2014
View

Summary

ApplicationWestern Blot
SpeciesHuman
Special ApplicationsWestern blot analysis of extracts from HeLa cells using TGN46 antibody (NBP1-49643, 1:100).
FileView PDF

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TGN46 Antibody (NBP1-49643) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-49643AF405
Alexa Fluor 488 Labeled NBP1-49643AF488
Alexa Fluor 647 Labeled NBP1-49643AF647
Alexa Fluor 700 Labeled NBP1-49643AF700
Biotin Labeled NBP1-49643B
DyLight 405 Labeled NBP1-49643V
DyLight 488 Labeled NBP1-49643G
DyLight 550 Labeled NBP1-49643R
DyLight 650 Labeled NBP1-49643C
FITC Labeled NBP1-49643F
HRP Labeled NBP1-49643H

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
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Anony- mous
06/12/2014
Application: ICC
Species: Human

Anony- mous
06/12/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol TGOLN2
Entrez
Uniprot