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TGN46 Antibody - BSA Free

Images

 
Genetic Strategies: Knockout Validated: TGN46 Antibody [NBP1-49643] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and TGN46 knockout (KO) HeLa cell line. PVDF ...read more
Immunocytochemistry/ Immunofluorescence: TGN46 Antibody [NBP1-49643] - A7 cells expressing F-NTPase were fixed and dual stained with an anti-FLAG antibody (green) and TGN46 antibody (red). Colocalization of F-NTPase ...read more
Immunocytochemistry/ Immunofluorescence: TGN46 Antibody [NBP1-49643] - U-2 OS cells were transfected with siRNAs targeting UGCG (siUGCG) or a non-targeting control (siNegCtrl) and plated onto glass coverslips. At 72 ...read more
Immunohistochemistry: TGN46 Antibody [NBP1-49643] - Analysis of TGOLN2 in human renal cell carcinoma.
Immunocytochemistry/ Immunofluorescence: TGN46 Antibody [NBP1-49643] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated ...read more
Flow Cytometry: TGN46 Antibody [NBP1-49643] - An intracellular stain was performed on HepG2 cells with NBP1-49643C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with ...read more
Western Blot: TGN46 Antibody [NBP1-49643] - Analysis of extracts from HeLa cells using TGN46 antibody (NBP1-49643, 1:100).
Immunocytochemistry/ Immunofluorescence: TGN46 Antibody [NBP1-49643] - HepG2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated ...read more
Immunocytochemistry/ Immunofluorescence: TGN46 Antibody [NBP1-49643] - Analysis of A549 cells using TGN46 antibody (NBP1-49643, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody ...read more
Immunocytochemistry/ Immunofluorescence: TGN46 Antibody [NBP1-49643] - T98G glioblastoma cells probed for Golgi (Alexa Fluor 488 conjugated TGN-46 antibody, Green), tubulin (Alexa Fluor 594, Red), and nucleus (DAPI, ...read more
Immunocytochemistry/ Immunofluorescence: TGN46 Antibody [NBP1-49643] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with ...read more
Flow (Intracellular): TGN46 Antibody [NBP1-49643] - An intracellular stain was performed on U-937 cells with NBP1-49643 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized ...read more
Flow (Intracellular): TGN46 Antibody [NBP1-49643] - An intracellular stain was performed on HepG2 cells with TGN46 Antibody NBP1-49643AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and ...read more
Nonvesicular fraction of GII-NTPase significantly localizes to the ER or mitochondria in cells. (B) A7 cells expressing F-NTPase were fixed and dual stained with an anti-FLAG antibody (green) and a set of antibodies or ...read more
The levels of glial fibrillary acidic protein (GFAP) and S100 beta as astrocytic protein marker proteins in the hippocampal area. (A) Western blot images using GFAP, tubulin beta‐3, and beta ‐actin antibodies to ...read more
Representative Western blots showing the IAP levels in EV derived from individual European American (EA) (N1-N5) & (N7-N9), *N6 and African American (AA) (N1-N9) patients with prostate cancer (PCa).Specific antibodies ...read more
Photomicrographs of immunostaining for osteocalcin and osteopontin in (A) Group I, (B) Group II (C) Group III at 6 weeks.The pictures are arranged by staining technique (columns) and the investigated treatment (rows). ...read more
TGN46 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Rabbit anti-TGN46 Affinity Purified Polyclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NBP1-49643AF647) (light ...read more

Product Details

Summary
Reactivity Hu, Mu, Bv, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, WB, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

TGN46 Antibody - BSA Free Summary

Immunogen
A genomic peptide made to an internal region of the human TGN46 protein (within residues 200-350). [Swiss-Prot: O43493]
Localization
Primarily in trans-Golgi network. Cycles between the trans-Golgi network and the cell surface returning via endosomes
Marker
TGN Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TGOLN2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunoblotting reported in scientific literature (PMID 25410859)
  • Immunocytochemistry/ Immunofluorescence reported in scientific literature (PMID 31455601)
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
  • Knockout Validated
  • Western Blot 2ug/mL
Application Notes
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reviewed Applications
Read 2 Reviews rated 5
using
NBP1-49643 in the following applications:

Publications
Read Publications using
NBP1-49643 in the following applications:

  • EM
    1 publication
  • 17 publications
  • IP
    1 publication
  • WB
    2 publications

Reactivity Notes

Monkey reactivity reported in scientific literature (PMID: 30135710). Mouse reactivity reported in scientific literature (PMID: 26980706). Bovine reactivity reported in scientific literature (PMID: 30135710).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for TGN46 Antibody - BSA Free

  • TGN 46
  • TGN 48
  • TGN 51
  • TGN38 homolog
  • TGN38
  • TGN-38
  • TGN46
  • TGN-46
  • TGN48
  • TGN51
  • TGOLN 2
  • TGOLN2
  • trans golgi network 38
  • trans golgi network 46
  • Trans Golgi network integral membrane protein 2 [Precursor]
  • Trans Golgi network integral membrane protein 2
  • Trans Golgi network protein (46 48 51kD isoforms)
  • Trans golgi network protein 2
  • Trans Golgi network protein TGN51
  • Trans-Golgi network integral membrane protein 2
  • Trans-Golgi network protein 2
  • Trans-Golgi network protein TGN51
  • TTGN 2
  • TTGN2

Background

TGN46 (TGOLN2/trans-Golgi network protein 2) is a single-pass type I membrane protein localized in cell membrane as well as in trans-Golgi network (TGN) membrane, an important protein sorting station inside cells. It shuttles between TGN and cell surface returning via endosomes. TGN46-vesicle formation requires protein kinase D, a kinase needed for secretory carrier formation in cells. TGOLN2 gene has several alternatively spliced transcripts, of which isoform TGN46 is widely expressed whereas another isoform TGN51 is more abundant in fetal lung and kidney. TGN46 is a heavily glycosylated protein with largely unknown functions, however, it has been suspected to be involved in regulating membrane traffic to and from the TGN. TGN46 contains a signal peptide, lumenal domain, membrane-spanning domain, and cytoplasmic domain. Because the membrane spanning region and cytoplasmic tail contain the retention and retrieval signals, respectively, for localization in the TGN, TGN46 is considered a molecular marker for TGN. Recent expression studies have shown a connection between TGOLN2 and suicidal behavior wherein TGN46 was found to be up-regulated in suicide completers as compared to controls.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TGN46 Antibody (NBP1-49643)(26)

We have publications tested in 3 confirmed species: Human, Bovine, Monkey.

We have publications tested in 4 applications: EM, ICC/IF, IP, WB.


Filter By Application
EM
(1)
ICC/IF
(17)
IP
(1)
WB
(2)
All Applications
Filter By Species
Human
(16)
Bovine
(1)
Monkey
(1)
All Species
Showing Publications 1 - 10 of 26. Show All 26 Publications.
Publications using NBP1-49643 Applications Species
Gu S, Maurya S, Lona A et al. Ligand-Dependent Mechanisms of C-C Chemokine Receptor 5 (CCR5) Trafficking Revealed by APEX2 Proximity Labeling Proteomics bioRxiv : the preprint server for biology 2023-11-03 [PMID: 37961097] (ICC/IF, Human) ICC/IF Human
Kim H, Lee Y, Lee S, Park B HCMV-encoded viral protein US12 promotes autophagy by inducing autophagy flux Biochemical and Biophysical Research Communications 2023-03-01 [PMID: 36898229] (ICC/IF) ICC/IF
Miller AN, Houlihan PR, Matamala E et al. The SARS-CoV-2 accessory protein Orf3a is not an ion channel, but does interact with trafficking proteins eLife 2023-01-25 [PMID: 36695574] (IP, EM) IP, EM
Steimle BL, Bailey DK, Smith FM Et al. Calcium and the Ca-ATPase SPCA1 modulate plasma membrane abundance of ZIP8 and ZIP14 to regulate Mn(II) uptake in brain microvascular endothelial cells J Biol Chem 2022-07-05 [PMID: 35787370] (ICC/IF, Human)

Details:
Citation using the Alexa Fluor 647 version of this antibody.
ICC/IF Human
Song P, Peng J, Omrane M et al. Septin 9 and phosphoinositides regulate lysosome localization and their association with lipid droplets iScience [PMID: 35573204]
Okada T, Nishida S, Zhang L Et al. Constitutive activation of S1P receptors at the trans-Golgi network is required for surface transport carrier formation iScience 2021-11-19 [PMID: 34805799] (ICC/IF, Human) ICC/IF Human
Kutchukian C, Vivas O, Casas M et al. NPC1 regulates the distribution of phosphatidylinositol 4-kinases at Golgi and lysosomal membranes The EMBO journal 2021-05-21 [PMID: 34019311]
Bakkar N, Starr A, Rabichow BE et al. The M1311V variant of ATP7A is associated with impaired trafficking and copper homeostasis in models of motor neuron disease Neurobiology of disease 2020-12-20 [PMID: 33359139] (ICC/IF) ICC/IF
Roy S, McCann CJ, Ralle M et al. Analysis of Wilson disease mutations revealed that interactions between different ATP7B mutants modify their properties Sci Rep 2020-08-10 [PMID: 32778786] (ICC/IF, Human) ICC/IF Human
McPhail JA, Lyoo H, Pemberton JG, Hoffmann RM Characterisation of the c10orf76-PI4KB complex, and its necessity for Golgi PI4P levels and enterovirus replication EMBO Rep 2019-12-13 [PMID: 31829496]
Show All 26 Publications.

Reviews for TGN46 Antibody (NBP1-49643) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NBP1-49643:
Filter by Applications
ICC
(1)
WB
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry TGN46 NBP1-49643
Enlarge
5
reviewed by:
Bryan Tinsley
ICC Human 06/12/2014
View

Summary

ApplicationImmunocytochemistry
SpeciesHuman
Western Blot TGN46 NBP1-49643
Enlarge
5
reviewed by:
Bryan Tinsley
WB Human 06/12/2014
View

Summary

ApplicationWestern Blot
SpeciesHuman

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TGN46 Antibody (NBP1-49643) (0)

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Secondary Antibodies

 

Isotype Controls

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Array NBP1-49643

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Blogs on TGN46

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Recent Reviews

5
5
2
4
0
3
0
2
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1
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Bryan Tinsley
06/12/2014
Application: ICC
Species: Human

Bryan Tinsley
06/12/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol TGOLN2
Entrez
Uniprot