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GCC185 Antibody

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Western Blot: GCC185 Antibody [NBP2-04024] - Detection of human GCC185 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-GCC185 antibody NBP2-04024 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. GCC185 was also immunoprecipitated by another rabbit anti-GCC185 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Independent Antibodies: Western Blot: GCC185 Antibody [NBP2-04024] - Detection of human GCC185 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% ...read more
Immunocytochemistry/ Immunofluorescence: GCC185 Antibody [NBP2-04024] - PC-3 cells (human) stained with GCC185 antibody (red). Nucleus stained with DAPI (blue). EVOS(TM) M5000 Imaging System - 63x oil objective. ICC/IF ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

        

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GCC185 Antibody Summary

Immunogen
The immunogen this antibody was made to, maps to a region between residue 1 and 50 of human 185 kDa Golgi coiled-coil protein using the numbering given in entry NP_852118.1 (GeneID 9648).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GCC2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000 - 1:10000
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation 2 - 10 ug/mg
Application Notes
This GCC185 antibody is validated for ICC/IF from a verified customer review.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-04024 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GCC185 Antibody

  • 185 kDa Golgi coiled-coil protein
  • CLL-associated antigen KW-11
  • CTCL tumor antigen se1-1
  • GCC protein, 185-kD
  • GCC185GRIP and coiled-coil domain-containing 2
  • Golgi coiled-coil protein GCC185
  • GRIP and coiled-coil domain containing 2
  • GRIP and coiled-coil domain-containing protein 2
  • KIAA0336REN53
  • Ran-binding protein 2-like 4
  • RANBP2L4
  • Renal carcinoma antigen NY-REN-53

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: TGN46 Antibody [NBP1-49643] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-TGN46 Antibody at 2 ug/ml for 60 minutes at room temperature and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective.
Immunocytochemistry/Immunofluorescence: TGN46 Antibody [NBP1-49643] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-TGN46 Antibody at 2 ug/ml for 60 minutes at room temperature and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
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Western Blot: RAB6A Antibody [NBP1-33110] - Wild-type (WT) and RAB6A knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB6A antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
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Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Thyroglobulin staining in thyroid tissue of normal C57 mouse (Red). Dilution is 1:100. This image was submitted via customer Review.
Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Analysis using Azide/BSA FREE version of NBP2-34294. Human Thyroid stained with Thyroglobulin Ab (2H11 + 6E1).
Immunohistochemistry: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Imaging of Mouse stomach tissue. Positive staining can only be observed inside the thyroid cells and the follicle in thyroid tissue. This image was submitted via customer Review.
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Western Blot: ARL1 Antibody [NBP2-15461] - Sample (30 ug of whole cell lysate) A: HUVEC 12% SDS PAGE gel, diluted at 1:1000.
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Immunocytochemistry/Immunofluorescence: Syntaxin 16 Antibody [NBP1-92467] - Staining of human cell line A-431 shows localization to vesicles. Antibody staining is shown in green.
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Immunohistochemistry-Paraffin: ARL4 Antibody [NBP3-03356] - Paraffin-embedded human liver cancer using ARL4 antibody at dilution of 1:100 (40x lens).
Immunocytochemistry/Immunofluorescence: ARL4 Antibody [NBP3-03356] - Analysis of NIH-3T3 cells using ARL4 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western Blot: STX10 Antibody [NBP2-85842] - Host: Rabbit. Target Name: STX10. Sample Tissue: Human Thymus Tumor lysates. Antibody Dilution: 1ug/ml
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Immunocytochemistry/Immunofluorescence: RAB6IP1 Antibody [NBP2-30981] - Staining of human cell line HeLa shows localization to the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RAB6IP1 Antibody [NBP2-30981] - Staining of human salivary gland shows distinct luminal membranous positivity in glandular duct cells.
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Publications for GCC185 Antibody (NBP2-04024) (0)

There are no publications for GCC185 Antibody (NBP2-04024).
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Review for GCC185 Antibody (NBP2-04024) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-04024:
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Immunofluorescence GCC185 NBP2-04024
Enlarge 		5
reviewed by:
Armen Petrosyan
 IF Human 08/28/2020
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Summary

ApplicationImmunofluorescence
Sample TestedPC-3 human prostate cancer cell line
SpeciesHuman
LotA1

Comments

CommentsPC-3 cells stained with GCC185 Ab - red, blue - nucleus, DAPI.

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FAQs for GCC185 Antibody (NBP2-04024) (0)

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Immunofluorescence GCC185 NBP2-04024
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 Armen Petrosyan
08/28/2020
Application: IF
Species: Human
read this review

Bioinformatics

Gene Symbol GCC2
Uniprot