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GCC185 Antibody

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Western Blot: GCC185 Antibody [NBP2-04024] - Detection of human GCC185 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-GCC185 antibody NBP2-04024 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. GCC185 was also immunoprecipitated by another rabbit anti-GCC185 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Independent Antibodies: Western Blot: GCC185 Antibody [NBP2-04024] - Detection of human GCC185 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% ...read more
Immunocytochemistry/ Immunofluorescence: GCC185 Antibody [NBP2-04024] - PC-3 cells (human) stained with GCC185 antibody (red). Nucleus stained with DAPI (blue). EVOS(TM) M5000 Imaging System - 63x oil objective. ICC/IF ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

        

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GCC185 Antibody Summary

Immunogen
The immunogen this antibody was made to, maps to a region between residue 1 and 50 of human 185 kDa Golgi coiled-coil protein using the numbering given in entry NP_852118.1 (GeneID 9648).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GCC2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation 2 - 10 ug/mg
  • Western Blot 1:2000 - 1:10000
Application Notes
This GCC185 antibody is validated for ICC/IF from a verified customer review. Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-04024 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GCC185 Antibody

  • 185 kDa Golgi coiled-coil protein
  • CLL-associated antigen KW-11
  • CTCL tumor antigen se1-1
  • GCC protein, 185-kD
  • GCC185GRIP and coiled-coil domain-containing 2
  • Golgi coiled-coil protein GCC185
  • GRIP and coiled-coil domain containing 2
  • GRIP and coiled-coil domain-containing protein 2
  • KIAA0336REN53
  • Ran-binding protein 2-like 4
  • RANBP2L4
  • Renal carcinoma antigen NY-REN-53

Background

GCC185 is encoded by this gene is a peripheral membrane protein localized to the trans-Golgi network. It is sensitive to brefeldin A. This encoded protein contains a GRIP domain which is thought to be used in targeting. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GCC185 Antibody (NBP2-04024) (0)

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Review for GCC185 Antibody (NBP2-04024) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-04024:
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Images Ratings Applications Species Date Details
Immunofluorescence GCC185 NBP2-04024
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reviewed by:
Verified Customer
 IF Human 08/28/2020
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Summary

ApplicationImmunofluorescence
Sample TestedPC-3 human prostate cancer cell line
SpeciesHuman
LotA1

Comments

CommentsPC-3 cells stained with GCC185 Ab - red, blue - nucleus, DAPI.

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Immunofluorescence GCC185 NBP2-04024
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 Verified Customer
08/28/2020
Application: IF
Species: Human
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Bioinformatics

Gene Symbol GCC2
Uniprot