TGN46 Antibody [Alexa Fluor® 488]

Images

 
Immunocytochemistry/ Immunofluorescence: TGN46 Antibody [Alexa Fluor® 488] [NBP1-49643AF488] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 ...read more
Immunocytochemistry/ Immunofluorescence: TGN46 Antibody [Alexa Fluor® 488] [NBP1-49643AF488] - HepG2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 ...read more
Flow (Intracellular): TGN46 Antibody [Alexa Fluor® 488] [NBP1-49643AF488] - TGN46 Antibody [Alexa Fluor 488] [NBP1-49643AF488] - An intracellular stain was performed on HepG2 cells with TGN46 Antibody ...read more
Immunocytochemistry/ Immunofluorescence: TGN46 Antibody [Alexa Fluor® 488] [NBP1-49643AF488] - TGN46 Antibody [Alexa Fluor 488] [NBP1-49643AF488] - T98G glioblastoma cells probed for Golgi (Alexa Fluor 488 ...read more
Immunocytochemistry/ Immunofluorescence: TGN46 Antibody [Alexa Fluor® 488] [NBP1-49643AF488] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% ...read more

Product Details

Summary
Reactivity Hu, Mu, Bv, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, WB, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Alexa Fluor 488

Order Details

TGN46 Antibody [Alexa Fluor® 488] Summary

Immunogen
A genomic peptide made to an internal region of the human TGN46 protein (within residues 200-350). [Swiss-Prot: O43493]
Localization
Primarily in trans-Golgi network. Cycles between the trans-Golgi network and the cell surface returning via endosomes
Marker
TGN Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TGOLN2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunoblotting
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockout Validated
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-49643AF488 in the following applications:

Reactivity Notes

Monkey reactivity reported in scientific literature (PMID: 30135710). Mouse reactivity reported in scientific literature (PMID: 26980706). Bovine reactivity reported in scientific literature (PMID: 30135710).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for TGN46 Antibody [Alexa Fluor® 488]

  • TGN 46
  • TGN 48
  • TGN 51
  • TGN38 homolog
  • TGN38
  • TGN-38
  • TGN46
  • TGN-46
  • TGN48
  • TGN51
  • TGOLN 2
  • TGOLN2
  • trans golgi network 38
  • trans golgi network 46
  • Trans Golgi network integral membrane protein 2 [Precursor]
  • Trans Golgi network integral membrane protein 2
  • Trans Golgi network protein (46 48 51kD isoforms)
  • Trans golgi network protein 2
  • Trans Golgi network protein TGN51
  • Trans-Golgi network integral membrane protein 2
  • Trans-Golgi network protein 2
  • Trans-Golgi network protein TGN51
  • TTGN 2
  • TTGN2

Background

The Trans-Golgi network integral membrane protein 2 (TGOLN2) was designated TGN46 based on the predicted molecular mass (46kDa). However, TGN46 is a heavily glycosylated protein, so its molecular weight is often detected at a 110-120kDa. The TGN46 protein is widely expressed. It may be involved in regulating membrane traffic to and from trans-Golgi network, and has been reported as the best available marker for human trans-Golgi network. TGN46 contains a luminal domain, a membrane-spanning domain, and a cytoplasmic domain. The membrane-spanning and cytoplasmic domains contain the retention and retrieval signals, respectively, for localization in the TGN.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TGN46 Antibody (NBP1-49643AF488) (0)

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Review for TGN46 Antibody (NBP1-49643AF488) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-49643AF488:
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ICC
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry TGN46 NBP1-49643AF488
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5
reviewed by:
Verified Customer
ICC Human 07/04/2017
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Summary

ApplicationImmunocytochemistry
Sample TestedT98G human glioblastoma cell line
SpeciesHuman
LotA-5-0090616-AF488

Comments

CommentsT98G glioblastoma cells were fixed with 4% paraformaldehyde. Upon permeabilization with ice-cold methanol, cells were washed 3X and blocked with goat serum. Samples were blocked using anti-TGN46 antibody overnight at 4C. Following morning, samples were washed thrice, probed using the anti-tubulin antibody for 1 hour. Following three successive washes, samples were mounted onto slides using ProLong Gold mountant with DAPI.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TGN46 Antibody (NBP1-49643AF488) (0)

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Secondary Antibodies

 

Isotype Controls

Additional TGN46 Products

Array NBP1-49643AF488

Research Areas for TGN46 Antibody (NBP1-49643AF488)

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Blogs on TGN46

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Verified Customer
07/04/2017
Application: ICC
Species: Human

Bioinformatics

Gene Symbol TGOLN2