TGN38 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: TGN38 Antibody [NBP1-03495] - Mouse embryonic fibroblasts, fixed with 4% PFA washed with PBS, permeabilized with 0.2% Triton X-100. Primary antibody at 1:100 in 3% BSA in PBS. ...read more
Immunocytochemistry/ Immunofluorescence: TGN38 Antibody [NBP1-03495] - Human Melanoma cell line C 32.
Immunocytochemistry/ Immunofluorescence: TGN38 Antibody [NBP1-03495] - Human Melanoma cell line C 32
Immunocytochemistry/ Immunofluorescence: TGN38 Antibody [NBP1-03495] - Human Melanoma cell line C 32 was cultured overnight on round cover slides placed in a 24 well tissue culture plate. Culture media removed and ...read more
Immunocytochemistry/ Immunofluorescence: TGN38 Antibody [NBP1-03495] - Human Melanoma cell line C 32 was cultured overnight on round cover slides placed in a 24 well tissue culture plate. Culture media removed and ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Catalog# & Conjugate Size Price

TGN38 Antibody Summary

Description
PLEASE NOTE: 0.1mg sample size is provided in reconstituted format and the 0.5mg size is provided lyophilized. (Please see reconstitution instuctions).
Immunogen
A synthetic peptide from the cytoplasmic part of mouse TGN38 conjugated to an immunogenic carrier protein was used as the immunogen.
Marker
TGN Marker
Specificity
TGN38 (or TGN46, TGN48, TGN51 in human).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TGOLN2
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:200
  • Immunocytochemistry/Immunofluorescence 1:50-1:250
  • Immunohistochemistry 1:50-1:250
Application Notes
The 0.1 mg size ships reconstituted and the 0.5 mg size ships lyophilized. ICC/IF reactivity reported in (PMID: 27505890).
Reviewed Applications
Read 1 Review rated 5
using
NBP1-03495 in the following applications:

Publications
Read Publications using
NBP1-03495 in the following applications:

  • 6 publications
  • WB
    3 publications

Reactivity Notes

Marmoset (100%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Contains 0.02% benzalkonium chloride.
Preservative
No Preservative
Purity
Ammonium sulfate precipitation
Reconstitution Instructions
Reconstitute with 0.5 ml of sterilized water to desired concentration. Centrifuge to remove any insoluble material. Note that the 0.1mg size ships already reconstituted.

Alternate Names for TGN38 Antibody

  • TGN38
  • TGN46
  • TGN46TGN38 homolog
  • TGN48MGC14722
  • TGN51
  • TGN5148, 51kD isoforms)
  • Tgoln1
  • Tgoln2
  • trans-golgi network protein 2
  • Trans-Golgi network protein TGN51
  • Ttgn1

Background

The trans-Golgi network (TGN) is part of the secretory pathway of eukaryotic cells which is distinct from the Golgi stack. The TGN is important in the later stages of protein secretion where it seems to play a key role in the sorting and targeting of secreted proteins to the correct destination. Some surface receptors recycle between the cell surface and the TGN, suggesting that the TGN is also important in endocytic pathways. TGN46 cycles between the trans-Golgi network and the cell surface returning via endosomes. It is thought to be involved in regulating membrane traffic to and from trans-Golgi network. Three splice isoforms are found: TGN46, TGN48 and TGN51. Isoform TGN46 is widely expressed, isoform TGN48 is barely expressed in embryonic kidney and promyelocytic cells whilst isoform TGN51 is more abundant in fetal lung and kidney. TGN46 is a human specific protein. The rodent homologue of TGN46 is known as TGN38.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TGN38 Antibody (NBP1-03495)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC/IF, WB.


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ICC/IF
(6)
WB
(3)
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Human
(2)
Mouse
(6)
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Showing Publications 1 - 10 of 10.
Publications using NBP1-03495 Applications Species
Watson AR, Dai H, Zheng Y et al. mTORC2 Deficiency Alters the Metabolic Profile of Conventional Dendritic Cells Front Immunol Jul 2 2019 [PMID: 31338091] (ICC/IF, Mouse) ICC/IF Mouse
Shi CS, Nabar NR, Huang NN, Kehrl JH SARS-Coronavirus Open Reading Frame-8b triggers intracellular stress pathways and activates NLRP3 inflammasomes Cell Death Discov Jun 5 2019 [PMID: 31231549] (ICC/IF) ICC/IF
Ansari IH, Longacre MJ, Stoker SW et al. Characterization of Acyl-CoA synthetase isoforms in pancreatic beta cells: Gene silencing shows participation of ACSL3 and ACSL4 in insulin secretion. Arch. Biochem. Biophys. 2017 Feb 11 [PMID: 28193492] (WB, Human) WB Human
Sugahara D Kobayashi Y Akimoto Y, Kawakami H. Mouse intestinal niche cells express a distinct a1,2-fucosylated glycan recognized by a lectin from Burkholderia cenocepacia. Glycobiology. Nov 22 2016 [PMID: 27979999] (Mouse) Mouse
Fernandez-Fernandez MR, Ruiz-Garcia D, Martin-Solana E et al. 3D electron tomography of brain tissue unveils distinct Golgi structures that sequester cytoplasmic contents in neurons. J. Cell. Sci. Aug 5 2016 [PMID: 27505890] (ICC/IF, Mouse) ICC/IF Mouse
Ansari IH, Longacre MJ, Paulusma CC et al. Characterization of P4 ATPase Phospholipid Translocases (Flippases) in Human and Rat Pancreatic Beta Cells: Their Gene Silencing Inhibits Insulin Secretion. J. Biol. Chem. 2015 Aug 03 [PMID: 26240149] (WB, Human) WB Human
Ting CH, Wen HL, Liu HC et al. The Spinal Muscular Atrophy Disease Protein SMN Is Linked to the Golgi Network PLoS One 2012 [PMID: 23284781] (ICC/IF, WB, Mouse) ICC/IF, WB Mouse
Petkovic M, Oses-Prieto JA, Burlingame A, Jan L TMEM16K is an interorganelle regulator of endosomal sorting bioRxiv Jan 1 2020
Fink, A;Mikulicic, S;Blaum, F;Reddehase, MJ;Florin, L;Lemmermann, NAW; Function of the cargo sorting dileucine motif in a cytomegalovirus immune evasion protein Med. Microbiol. Immunol. Apr 19 2019 [PMID: 31004199] (ICC/IF, Mouse) ICC/IF Mouse
Ren C, Yuan Q, Braun M et al. Leukocyte Cytoskeleton Polarization Is Initiated by Plasma Membrane Curvature from Cell Attachment Dev. Cell Mar 23 2019 [PMID: 30930167] (ICC/IF, Mouse) ICC/IF Mouse

Review for TGN38 Antibody (NBP1-03495) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-03495:
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Images Ratings Applications Species Date Details
 TGN38 NBP1-03495
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5
reviewed by:
Verified Customer
Mouse 08/05/2019
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Summary

Sample TestedMEF
SpeciesMouse
LotRb0705-060908-WS

Comments

CommentsMEF cells were fixed by 4% PFA in PBS, for 15 min at room temperature. The cells were washed with PBS, and permeabilized with 0.2% Triton X100 in PBS, for 5 min at room temperature. The cells were washed with PBS, and then incubated with the indicated antibodies diluted as 1:100 in 3% BSA in PBS, at 4 degree overnight. On the following day, the cells were washed with PBS, and incubated with corresponding secondary antibodies, for 2 h at room temperature. Then the cells were washed with PBS, and mounted with moviol.

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TGN38 Antibody (NBP1-03495) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 550 NBP1-03495R
DyLight 755 NBP1-03495IR

Additional TGN38 Products

Array NBP1-03495

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Verified Customer
08/05/2019
Application:
Species: Mouse

Bioinformatics

Gene Symbol TGOLN2
Entrez
Uniprot